# 2020/02/12 10:21:11 # HUMAN Target (Host) organism # RNA Sequencing source # ERR188195 Replication ID # ERR188195 Sample ID # ERR188195_1 Read1 # ERR188195_2 Read2 R1 26410795 (100.0000%) [A] Total raw reads R1 25889895 (98.0277%) [B] Total reads after QC of (A) R1 R1 25889895 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 48332088 (93.3416%) [B-3] Total mapped reads of (B-2)x2 R1 3447702 (6.6584%) [B-4] Total unmapped reads of (B-2)x2 R1 24205949 (93.4957%) [F] Total mapped reads of (B-2) R1 1683946 (6.5043%) [G] Total unmapped reads of (B-2) R1 217693 (0.8408%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 25672202 (99.1592%) [J] Total mapped reads to host genome ( (B-2)-H) R1 164027 (0.6336%) [I-1] Total mapped reads of (H) to exo. genomes R1 6389.284 [I-3] RPMH of (I-1) R1 6335.561 [I-4] PPM of (I-1) R1 753478.522 [I-5] RPMU of (I-1) R1 280 (0.0011%) [I-6] Total chimeric reads of (I-1) R1 10.907 [I-7] RPMH of (I-6) R1 10.815 [I-8] PPM of (I-6) R1 1286.215 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 26410795 (100.0000%) [A] Total raw reads R2 25889895 (98.0277%) [B] Total reads after QC of (A) R2 R2 25889895 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 48332088 (93.3416%) [B-3] Total mapped reads of (B-2)x2 R2 3447702 (6.6584%) [B-4] Total unmapped reads of (B-2)x2 R2 24126139 (93.1875%) [F] Total mapped reads of (B-2) R2 1763756 (6.8125%) [G] Total unmapped reads of (B-2) R2 247670 (0.9566%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 25642225 (99.0434%) [J] Total mapped reads to host genome ( (B-2)-H) R2 163211 (0.6304%) [I-1] Total mapped reads of (H) to exo. genomes R2 6364.931 [I-3] RPMH of (I-1) R2 6304.043 [I-4] PPM of (I-1) R2 658985.747 [I-5] RPMU of (I-1) R2 299 (0.0012%) [I-6] Total chimeric reads of (I-1) R2 11.661 [I-7] RPMH of (I-6) R2 11.549 [I-8] PPM of (I-6) R2 1207.252 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 79.0381 78.3735 9320.8371 2215 645 1570 2029.081 165257 ALL_R1 bacteria Nocardia 0.0 15.9480 15.8139 1880.7219 447 62 385 409.420 32815 ALL_R1 bacteria Escherichia 0.0 4.9528 4.9111 584.0748 148 26 122 127.149 10971 ALL_R1 bacteria Staphylococcus 1.50e-14 << 162.2949 160.9302 19139.1868 4358 312 4046 4166.467 323209 ALL_R1 fungi Saccharomyces 0.0 << 3724.1449 3692.8307 439182.7022 95607 0 95607 95607.000 7071623 ALL_R1 viral Lymphocryptovirus 0.0 << 72.7265 72.0308 7529.6564 2215 1361 854 1864.870 164433 ALL_R2 bacteria Nocardia 0.0 15.3486 15.2018 1589.0984 432 65 367 393.572 30865 ALL_R2 bacteria Escherichia 0.0 4.9869 4.9391 516.3080 153 32 121 127.874 11372 ALL_R2 bacteria Staphylococcus 2.16e-14 << 162.4327 160.8788 16817.2770 4349 318 4031 4165.135 322805 ALL_R2 fungi Saccharomyces 0.0 << 3729.6685 3693.9895 386146.8890 95637 0 95637 95637.000 7045659 ALL_R2 viral Lymphocryptovirus 0.0 331.6101 328.4379 34332.8704 59305 59304 1 8503.222 4303652 ALL_R2 viral Phix174microvirus n/a << 0.0390 0.0386 4.5936 1 0 1 1.000 72 R1U bacteria Cutibacterium n/a << 0.0417 0.0413 4.9152 3 2 1 1.070 183 R1U fungi Saccharomyces n/a 0.0417 0.0413 4.9152 3 2 1 1.070 163 R1U fungi Lodderomyces n/a 0.0407 0.0404 4.8003 2 1 1 1.045 121 R1U fungi Paracoccidioides n/a << 8.1411 8.0726 960.0676 209 0 209 209.000 15378 R1U viral Lymphocryptovirus n/a 0.0779 0.0773 9.1872 2 0 2 2.000 113 R1U viral Betaretrovirus n/a << 0.0981 0.0972 10.1587 3 2 1 2.516 193 R2U bacteria Nocardia n/a 0.0391 0.0387 4.0457 2 1 1 1.002 116 R2U bacteria Nitratireductor n/a 0.0390 0.0386 4.0376 1 0 1 1.000 36 R2U bacteria Polaromonas n/a << 0.1087 0.1077 11.2569 5 3 2 2.788 346 R2U fungi Saccharomyces n/a 0.0856 0.0847 8.8586 3 2 1 2.194 114 R2U fungi Auricularia n/a 0.0781 0.0773 8.0833 3 1 2 2.002 186 R2U fungi Spizellomyces n/a 0.0697 0.0691 7.2193 4 3 1 1.788 258 R2U fungi Schizosaccharomyces n/a 0.0561 0.0555 5.8061 3 2 1 1.438 164 R2U fungi Paracoccidioides n/a 0.0402 0.0398 4.1588 3 2 1 1.030 198 R2U fungi Setosphaeria n/a 0.0402 0.0398 4.1588 3 2 1 1.030 193 R2U fungi Ustilago n/a 0.0391 0.0387 4.0457 2 1 1 1.002 128 R2U fungi Sporothrix n/a 0.0391 0.0387 4.0457 2 1 1 1.002 120 R2U fungi Neofusicoccum n/a 0.0391 0.0387 4.0457 2 1 1 1.002 117 R2U fungi Sordaria n/a 0.0390 0.0386 4.0376 1 0 1 1.000 51 R2U fungi Pochonia n/a 0.0390 0.0386 4.0376 1 0 1 1.000 40 R2U fungi Podospora n/a << 9.6715 9.5790 1001.3324 248 0 248 248.000 17975 R2U viral Lymphocryptovirus n/a 0.0390 0.0386 4.0376 1 0 1 1.000 50 R2U viral Betaretrovirus n/a