# 2020/03/04 18:19:38 # HUMAN Target (Host) organism # DNA Sequencing source # SRR792326 Replication ID # SRP000547 Sample ID # SRR792326_1.filt Read1 # SRR792326_2.filt Read2 R1 9892204 (100.0000%) [A] Total raw reads R1 7649362 (77.3272%) [B] Total reads after QC of (A) R1 R1 7649362 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 13939939 (91.1183%) [B-3] Total mapped reads of (B-2)x2 R1 1358785 (8.8817%) [B-4] Total unmapped reads of (B-2)x2 R1 7070263 (92.4294%) [F] Total mapped reads of (B-2) R1 579099 (7.5706%) [G] Total unmapped reads of (B-2) R1 67205 (0.8786%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 7582157 (99.1214%) [J] Total mapped reads to host genome ( (B-2)-H) R1 1717 (0.0224%) [I-1] Total mapped reads of (H) to exo. genomes R1 226.453 [I-3] RPMH of (I-1) R1 224.463 [I-4] PPM of (I-1) R1 25548.694 [I-5] RPMU of (I-1) R1 9 (0.0001%) [I-6] Total chimeric reads of (I-1) R1 1.187 [I-7] RPMH of (I-6) R1 1.177 [I-8] PPM of (I-6) R1 133.919 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 9892204 (100.0000%) [A] Total raw reads R2 7649362 (77.3272%) [B] Total reads after QC of (A) R2 R2 7649362 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 13939939 (91.1183%) [B-3] Total mapped reads of (B-2)x2 R2 1358785 (8.8817%) [B-4] Total unmapped reads of (B-2)x2 R2 6869676 (89.8072%) [F] Total mapped reads of (B-2) R2 779686 (10.1928%) [G] Total unmapped reads of (B-2) R2 147698 (1.9309%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 7501664 (98.0691%) [J] Total mapped reads to host genome ( (B-2)-H) R2 1754 (0.0229%) [I-1] Total mapped reads of (H) to exo. genomes R2 233.815 [I-3] RPMH of (I-1) R2 229.300 [I-4] PPM of (I-1) R2 11875.584 [I-5] RPMU of (I-1) R2 18 (0.0002%) [I-6] Total chimeric reads of (I-1) R2 2.400 [I-7] RPMH of (I-6) R2 2.353 [I-8] PPM of (I-6) R2 121.870 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1.5784 1.5646 178.0820 22 16 6 11.968 1357 ALL_R1 bacteria Alcanivorax 1.03e-09 0.2638 0.2615 29.7597 2 0 2 2.000 185 ALL_R1 bacteria Sphingomonas 3.80e-04 << 220.2539 218.3189 24849.3416 1670 0 1670 1670.000 154911 ALL_R1 viral Lymphocryptovirus 0.0 << 4.2787 4.1960 217.3151 57 40 17 32.097 3908 ALL_R2 bacteria Alcanivorax 1.08e-72 2.5606 2.5112 130.0559 41 35 6 19.209 2764 ALL_R2 bacteria Tetrasphaera 2.74e-05 0.2666 0.2615 13.5411 2 0 2 2.000 159 ALL_R2 bacteria Sphingomonas 5.02e-04 << 222.6173 218.3189 11306.8559 1670 0 1670 1670.000 151673 ALL_R2 viral Lymphocryptovirus 0.0 << 1.1870 1.1766 133.9186 9 0 9 9.000 895 R1U viral Lymphocryptovirus n/a << 0.2339 0.2294 11.8824 3 2 1 1.755 167 R2U bacteria Alcanivorax n/a 0.1333 0.1307 6.7706 1 0 1 1.000 38 R2U bacteria Leptolyngbya n/a 0.1333 0.1307 6.7706 1 0 1 1.000 38 R2U bacteria Escherichia n/a 0.1333 0.1307 6.7706 1 0 1 1.000 36 R2U bacteria Dysgonomonas n/a << 1.5996 1.5688 81.2469 12 0 12 12.000 968 R2U viral Lymphocryptovirus n/a