# 2017/12/23 07:47:48 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR000AAP_1 Replication ID # ENCSR000AAP Sample ID # ENCFF001RBG Read1 # ENCFF001RBF Read2 R1 208742663 (100.0000%) [A] Total raw reads R1 194807913 (93.3244%) [B] Total reads after QC of (A) R1 R1 194807913 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 334838651 (85.9407%) [B-3] Total mapped reads of (B-2)x2 R1 54777175 (14.0593%) [B-4] Total unmapped reads of (B-2)x2 R1 167125385 (85.7898%) [F] Total mapped reads of (B-2) R1 27682528 (14.2102%) [G] Total unmapped reads of (B-2) R1 10540174 (5.4105%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 184267739 (94.5895%) [J] Total mapped reads to host genome ( (B-2)-H) R1 5908144 (3.0328%) [I-1] Total mapped reads of (H) to exo. genomes R1 32062.823 [I-3] RPMH of (I-1) R1 30328.049 [I-4] PPM of (I-1) R1 988 (0.0005%) [I-5] Total chimeric reads of (I-1) R1 5.362 [I-7] RPMH of (I-5) R1 5.072 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 208742663 (100.0000%) [A] Total raw reads R2 194807913 (93.3244%) [B] Total reads after QC of (A) R2 R2 194807913 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 334838651 (85.9407%) [B-3] Total mapped reads of (B-2)x2 R2 54777175 (14.0593%) [B-4] Total unmapped reads of (B-2)x2 R2 167713266 (86.0916%) [F] Total mapped reads of (B-2) R2 27094647 (13.9084%) [G] Total unmapped reads of (B-2) R2 10601311 (5.4419%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 184206602 (94.5581%) [J] Total mapped reads to host genome ( (B-2)-H) R2 5898642 (3.0279%) [I-1] Total mapped reads of (H) to exo. genomes R2 32021.882 [I-3] RPMH of (I-1) R2 30279.273 [I-4] PPM of (I-1) R2 396 (0.0002%) [I-5] Total chimeric reads of (I-1) R2 2.150 [I-7] RPMH of (I-5) R2 2.033 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 7032.5761 6652.0753 1311364 43211 1268153 1295876.898 124577694 ALL_R1 bacteria Bacillus 0.0 5313.4352 5025.9493 1034868 217097 817771 979094.690 96185931 ALL_R1 bacteria Burkholderia 0.0 3728.9865 3527.2279 3221672 3191840 29832 687131.906 312323461 ALL_R1 bacteria Pseudomonas 0.0 981.6616 928.5484 197113 60413 136700 180888.568 18696486 ALL_R1 bacteria Afipia 0.0 247.7351 234.3313 62082 54775 7307 45649.588 5783790 ALL_R1 bacteria Bradyrhizobium 0.0 140.8901 133.2672 51776 48519 3257 25961.507 4805289 ALL_R1 bacteria Cupriavidus 3.31e-17 135.9256 128.5713 41162 28038 13124 25046.704 3836780 ALL_R1 bacteria Variovorax 0.0 106.6958 100.9230 30967 29616 1351 19660.602 2924873 ALL_R1 bacteria Alicycliphilus 3.56e-05 88.8610 84.0532 44600 37908 6692 16374.221 4329857 ALL_R1 bacteria Escherichia 0.0 61.3149 57.9974 27386 22742 4644 11298.350 2557854 ALL_R1 bacteria Herbaspirillum 1.09e-116 33.0726 31.2832 16617 13490 3127 6094.210 1604748 ALL_R1 bacteria Cutibacterium 4.79e-14 << 3526.6159 3335.8067 3167909 3167906 3 649841.535 307344488 ALL_R1 viral Phix174microvirus n/a << 7083.3894 6697.9163 1318042 23293 1294749 1304807.094 129165168 ALL_R2 bacteria Bacillus 0.0 5319.3286 5029.8544 1030952 213708 817244 979855.443 97983983 ALL_R2 bacteria Burkholderia 0.0 3623.9643 3426.7507 3219801 3190068 29733 667558.152 309063576 ALL_R2 bacteria Pseudomonas 0.0 982.5021 929.0350 195836 59415 136421 180983.368 18847413 ALL_R2 bacteria Afipia 0.0 248.2419 234.7327 60628 53356 7272 45727.794 5741010 ALL_R2 bacteria Bradyrhizobium 0.0 146.3150 138.3526 51150 47890 3260 26952.181 4833742 ALL_R2 bacteria Cupriavidus 1.90e-17 137.6128 130.1240 40472 27379 13093 25349.186 3834589 ALL_R2 bacteria Variovorax 0.0 108.4858 102.5820 30788 29441 1347 19983.792 2947061 ALL_R2 bacteria Alicycliphilus 3.97e-05 91.4871 86.5085 41847 35271 6576 16852.536 4065340 ALL_R2 bacteria Escherichia 0.0 64.0876 60.6000 27092 22457 4635 11805.354 2564034 ALL_R2 bacteria Herbaspirillum 1.51e-116 << 3398.7173 3213.7615 3151123 3151116 7 626066.163 302604466 ALL_R2 viral Phix174microvirus n/a << 1.1505 1.0883 212 0 212 212.000 17511 R1U bacteria Bacillus n/a RN7SL1(29);RN7SL2(10);RMRP(7);RN7SK(5);ANKRD28(4)