# 2018/02/09 05:20:17 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR000CUB_2 Replication ID # ENCSR000CUB Sample ID # ENCFF000GBQ Read1 # ENCFF000GBU Read2 R1 163854307 (100.0000%) [A] Total raw reads R1 160214970 (97.7789%) [B] Total reads after QC of (A) R1 R1 160214970 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 294161897 (91.8023%) [B-3] Total mapped reads of (B-2)x2 R1 26268043 (8.1977%) [B-4] Total unmapped reads of (B-2)x2 R1 148201447 (92.5016%) [F] Total mapped reads of (B-2) R1 12013523 (7.4984%) [G] Total unmapped reads of (B-2) R1 4809861 (3.0021%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 155405109 (96.9979%) [J] Total mapped reads to host genome ( (B-2)-H) R1 3961089 (2.4724%) [I-1] Total mapped reads of (H) to exo. genomes R1 25488.795 [I-3] RPMH of (I-1) R1 24723.589 [I-4] PPM of (I-1) R1 1290 (0.0008%) [I-5] Total chimeric reads of (I-1) R1 8.301 [I-7] RPMH of (I-5) R1 8.052 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 163854307 (100.0000%) [A] Total raw reads R2 160214970 (97.7789%) [B] Total reads after QC of (A) R2 R2 160214970 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 294161897 (91.8023%) [B-3] Total mapped reads of (B-2)x2 R2 26268043 (8.1977%) [B-4] Total unmapped reads of (B-2)x2 R2 145960450 (91.1029%) [F] Total mapped reads of (B-2) R2 14254520 (8.8971%) [G] Total unmapped reads of (B-2) R2 5718938 (3.5695%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 154496032 (96.4305%) [J] Total mapped reads to host genome ( (B-2)-H) R2 3935796 (2.4566%) [I-1] Total mapped reads of (H) to exo. genomes R2 25475.062 [I-3] RPMH of (I-1) R2 24565.719 [I-4] PPM of (I-1) R2 973 (0.0006%) [I-5] Total chimeric reads of (I-1) R2 6.298 [I-7] RPMH of (I-5) R2 6.073 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 4603.7405 4465.5302 2128607 2065294 63313 715444.795 209120643 ALL_R1 bacteria Paracoccus 0.0 890.0786 863.3573 182480 48679 133801 138322.758 17514035 ALL_R1 bacteria Bacillus 0.0 15.9664 15.4871 15750 13821 1929 2481.266 1238147 ALL_R1 bacteria Staphylococcus 4.71e-07 << 434.1232 421.0902 68405 4875 63530 67464.956 6745531 ALL_R1 fungi Saccharomyces 0.0 << 6983.0113 6773.3722 1577836 1577833 3 1085195.631 155136974 ALL_R1 viral Phix174microvirus n/a << 5830.5533 5622.4294 2115503 2050257 65246 900797.353 201590585 ALL_R2 bacteria Paracoccus 0.0 894.0993 862.1841 171842 37128 134714 138134.792 16143180 ALL_R2 bacteria Bacillus 0.0 14.0418 13.5406 6181 4296 1885 2169.405 539076 ALL_R2 bacteria Staphylococcus 4.18e-06 << 431.0256 415.6399 67823 4709 63114 66591.740 6762466 ALL_R2 fungi Saccharomyces 0.0 << 5468.0702 5272.8853 1562454 1562449 5 844795.154 154907370 ALL_R2 viral Phix174microvirus n/a << 2.5550 2.4783 465 214 251 397.055 37706 R1U bacteria Mycobacterium n/a RN7SL1(182);RN7SL2(53);RNR2(6);ANKRD28(5);COL1A2(3) 2.2843 2.2157 723 679 44 354.987 69055 R1U bacteria Paracoccus n/a RN7SL2(70);RN7SL1(41);RNR2(35);THBS1(27);ANKRD28(15) << 2.0444 1.9714 778 736 42 315.853 74299 R2U bacteria Paracoccus n/a RNR2(67);RN7SL2(49);RMRP(16);RN7SL1(16);THBS1(13)