# 2018/02/09 17:26:17 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR000CUJ_2 Replication ID # ENCSR000CUJ Sample ID # ENCFF000FJX Read1 # ENCFF000FJY Read2 R1 190902375 (100.0000%) [A] Total raw reads R1 186163609 (97.5177%) [B] Total reads after QC of (A) R1 R1 186163609 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 344621089 (92.5587%) [B-3] Total mapped reads of (B-2)x2 R1 27706129 (7.4413%) [B-4] Total unmapped reads of (B-2)x2 R1 172168651 (92.4824%) [F] Total mapped reads of (B-2) R1 13994958 (7.5176%) [G] Total unmapped reads of (B-2) R1 4776453 (2.5657%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 181387156 (97.4343%) [J] Total mapped reads to host genome ( (B-2)-H) R1 1885455 (1.0128%) [I-1] Total mapped reads of (H) to exo. genomes R1 10394.645 [I-3] RPMH of (I-1) R1 10127.946 [I-4] PPM of (I-1) R1 435 (0.0002%) [I-5] Total chimeric reads of (I-1) R1 2.398 [I-7] RPMH of (I-5) R1 2.337 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 190902375 (100.0000%) [A] Total raw reads R2 186163609 (97.5177%) [B] Total reads after QC of (A) R2 R2 186163609 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 344621089 (92.5587%) [B-3] Total mapped reads of (B-2)x2 R2 27706129 (7.4413%) [B-4] Total unmapped reads of (B-2)x2 R2 172452438 (92.6349%) [F] Total mapped reads of (B-2) R2 13711171 (7.3651%) [G] Total unmapped reads of (B-2) R2 4982277 (2.6763%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 181181332 (97.3237%) [J] Total mapped reads to host genome ( (B-2)-H) R2 1894158 (1.0175%) [I-1] Total mapped reads of (H) to exo. genomes R2 10454.488 [I-3] RPMH of (I-1) R2 10174.695 [I-4] PPM of (I-1) R2 541 (0.0003%) [I-5] Total chimeric reads of (I-1) R2 2.986 [I-7] RPMH of (I-5) R2 2.906 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2991.4257 2914.6738 629444 334647 294797 542606.201 60365589 ALL_R1 bacteria Bosea 0.0 1685.0258 1641.7926 386986 308782 78204 305642.030 37164503 ALL_R1 bacteria Methylobacterium 0.0 1278.0243 1245.2337 235489 4804 230685 231817.192 23355373 ALL_R1 bacteria Bacillus 0.0 145.6332 141.8967 66469 64962 1507 26415.995 6277306 ALL_R1 bacteria Bradyrhizobium 1.39e-127 89.5930 87.2943 43827 43038 789 16251.024 4191870 ALL_R1 bacteria Chelatococcus 2.00e-04 44.5695 43.4260 26236 25428 808 8084.340 2494068 ALL_R1 bacteria Prosthecomicrobium 4.11e-21 8.4075 8.1918 2456 1124 1332 1525.011 244797 ALL_R1 bacteria Staphylococcus 3.35e-68 << 252.0120 245.5460 47117 3445 43672 45711.731 4694208 ALL_R1 fungi Saccharomyces 0.0 << 3021.6681 2940.7996 635432 339666 295766 547469.859 60048068 ALL_R2 bacteria Bosea 0.0 1702.8775 1657.3035 390543 311922 78621 308529.606 36967090 ALL_R2 bacteria Methylobacterium 0.0 1308.4885 1273.4695 239912 3961 235951 237073.686 23867851 ALL_R2 bacteria Bacillus 0.0 157.6839 153.4639 70321 68611 1710 28569.385 6497850 ALL_R2 bacteria Bradyrhizobium 1.74e-198 93.3153 90.8179 45177 44396 781 16906.985 4247263 ALL_R2 bacteria Chelatococcus 7.45e-04 47.9241 46.6415 27487 26610 877 8682.956 2568220 ALL_R2 bacteria Prosthecomicrobium 1.24e-30 8.5444 8.3157 2536 1187 1349 1548.080 251884 ALL_R2 bacteria Staphylococcus 1.16e-70 << 251.2037 244.4807 47111 3420 43691 45513.416 4703563 ALL_R2 fungi Saccharomyces 0.0 << 803.4820 781.9785 822374 822373 1 145575.933 80988813 ALL_R2 viral Phix174microvirus n/a << 1.0170 0.9898 190 6 184 184.264 18703 R2U bacteria Bacillus n/a RNR2(26);MASP2(6);NAV2(6);CCND2(5);TUBB3(5)