# 2020/03/07 00:46:43 # HUMAN Target (Host) organism # DNA Sequencing source # SRR035017 Replication ID # SRP000547 Sample ID # SRR035017_1.filt Read1 # SRR035017_2.filt Read2 R1 20097532 (100.0000%) [A] Total raw reads R1 17560904 (87.3784%) [B] Total reads after QC of (A) R1 R1 17560904 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 32380987 (92.1962%) [B-3] Total mapped reads of (B-2)x2 R1 2740821 (7.8038%) [B-4] Total unmapped reads of (B-2)x2 R1 16200649 (92.2541%) [F] Total mapped reads of (B-2) R1 1360255 (7.7459%) [G] Total unmapped reads of (B-2) R1 139480 (0.7943%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 17421424 (99.2057%) [J] Total mapped reads to host genome ( (B-2)-H) R1 5029 (0.0286%) [I-1] Total mapped reads of (H) to exo. genomes R1 288.668 [I-3] RPMH of (I-1) R1 286.375 [I-4] PPM of (I-1) R1 36055.348 [I-5] RPMU of (I-1) R1 68 (0.0004%) [I-6] Total chimeric reads of (I-1) R1 3.903 [I-7] RPMH of (I-6) R1 3.872 [I-8] PPM of (I-6) R1 487.525 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 20097532 (100.0000%) [A] Total raw reads R2 17560904 (87.3784%) [B] Total reads after QC of (A) R2 R2 17560904 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 32380987 (92.1962%) [B-3] Total mapped reads of (B-2)x2 R2 2740821 (7.8038%) [B-4] Total unmapped reads of (B-2)x2 R2 16180338 (92.1384%) [F] Total mapped reads of (B-2) R2 1380566 (7.8616%) [G] Total unmapped reads of (B-2) R2 140448 (0.7998%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 17420456 (99.2002%) [J] Total mapped reads to host genome ( (B-2)-H) R2 5059 (0.0288%) [I-1] Total mapped reads of (H) to exo. genomes R2 290.406 [I-3] RPMH of (I-1) R2 288.083 [I-4] PPM of (I-1) R2 36020.449 [I-5] RPMU of (I-1) R2 70 (0.0004%) [I-6] Total chimeric reads of (I-1) R2 4.018 [I-7] RPMH of (I-6) R2 3.986 [I-8] PPM of (I-6) R2 498.405 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1.5242 1.5121 190.3785 51 39 12 26.554 3711 ALL_R1 bacteria Eubacterium 1.53e-09 1.4814 1.4696 185.0301 49 37 12 25.808 3514 ALL_R1 bacteria Alcanivorax 3.38e-11 1.2878 1.2776 160.8474 45 36 9 22.435 3160 ALL_R1 bacteria Campylobacter 2.53e-04 << 281.6647 279.4275 35180.6711 4907 0 4907 4907.000 354215 ALL_R1 viral Lymphocryptovirus 0.0 << 2.5427 2.5224 315.3836 83 62 21 44.295 5827 ALL_R2 bacteria Alcanivorax 3.06e-37 1.5086 1.4965 187.1155 55 43 12 26.280 3974 ALL_R2 bacteria Eubacterium 1.95e-09 1.2501 1.2401 155.0538 48 39 9 21.777 3470 ALL_R2 bacteria Campylobacter 2.80e-04 << 281.1637 278.9150 34874.1171 4898 0 4898 4898.000 349923 ALL_R2 viral Lymphocryptovirus 0.0 << 3.7884 3.7583 473.1861 66 0 66 66.000 4593 R1U viral Lymphocryptovirus n/a << 0.0655 0.0650 8.1240 2 1 1 1.141 109 R2U bacteria Campylobacter n/a << 3.7886 3.7583 469.9248 66 0 66 66.000 4910 R2U viral Lymphocryptovirus n/a 0.0574 0.0569 7.1201 1 0 1 1.000 40 R2U viral Orthopoxvirus n/a