# 2018/02/08 02:20:18 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR000CUE_2 Replication ID # ENCSR000CUE Sample ID # ENCFF000FMM Read1 # ENCFF000FMN Read2 R1 179807338 (100.0000%) [A] Total raw reads R1 174661047 (97.1379%) [B] Total reads after QC of (A) R1 R1 174661047 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 329052776 (94.1975%) [B-3] Total mapped reads of (B-2)x2 R1 20269318 (5.8025%) [B-4] Total unmapped reads of (B-2)x2 R1 164581990 (94.2294%) [F] Total mapped reads of (B-2) R1 10079057 (5.7706%) [G] Total unmapped reads of (B-2) R1 2253352 (1.2901%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 172407695 (98.7099%) [J] Total mapped reads to host genome ( (B-2)-H) R1 901801 (0.5163%) [I-1] Total mapped reads of (H) to exo. genomes R1 5230.631 [I-3] RPMH of (I-1) R1 5163.149 [I-4] PPM of (I-1) R1 466 (0.0003%) [I-5] Total chimeric reads of (I-1) R1 2.703 [I-7] RPMH of (I-5) R1 2.668 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 179807338 (100.0000%) [A] Total raw reads R2 174661047 (97.1379%) [B] Total reads after QC of (A) R2 R2 174661047 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 329052776 (94.1975%) [B-3] Total mapped reads of (B-2)x2 R2 20269318 (5.8025%) [B-4] Total unmapped reads of (B-2)x2 R2 164470786 (94.1657%) [F] Total mapped reads of (B-2) R2 10190261 (5.8343%) [G] Total unmapped reads of (B-2) R2 2488053 (1.4245%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 172172994 (98.5755%) [J] Total mapped reads to host genome ( (B-2)-H) R2 906537 (0.5190%) [I-1] Total mapped reads of (H) to exo. genomes R2 5265.268 [I-3] RPMH of (I-1) R2 5190.264 [I-4] PPM of (I-1) R2 447 (0.0003%) [I-5] Total chimeric reads of (I-1) R2 2.596 [I-7] RPMH of (I-5) R2 2.559 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1112.6476 1098.2930 224201 118593 105608 191829.000 21033005 ALL_R1 bacteria Bosea 0.0 1068.6406 1054.8538 186505 2980 183525 184241.864 18320496 ALL_R1 bacteria Bacillus 0.0 617.4381 609.4723 136537 108522 28015 106451.075 12833495 ALL_R1 bacteria Methylobacterium 0.0 54.5419 53.8383 24450 23839 611 9403.450 2242653 ALL_R1 bacteria Bradyrhizobium 7.78e-66 5.0744 5.0089 1482 747 735 874.860 146630 ALL_R1 bacteria Staphylococcus 5.29e-111 << 143.2308 141.3829 25624 2015 23609 24694.094 2532635 ALL_R1 fungi Saccharomyces 0.0 << 1149.6880 1133.3107 232388 124391 107997 197945.228 21599286 ALL_R2 bacteria Bosea 0.0 1079.6179 1064.2387 187847 2737 185110 185881.043 18601011 ALL_R2 bacteria Bacillus 0.0 641.8215 632.6787 142499 113785 28714 110504.324 13278750 ALL_R2 bacteria Methylobacterium 0.0 58.9699 58.1299 26231 25606 625 10153.025 2383064 ALL_R2 bacteria Bradyrhizobium 3.54e-71 17.2440 16.9984 9958 9635 323 2968.958 917262 ALL_R2 bacteria Prosthecomicrobium 3.16e-07 5.0851 5.0127 1461 725 736 875.516 144006 ALL_R2 bacteria Staphylococcus 2.30e-109 << 143.1021 141.0636 25533 1889 23644 24638.320 2540807 ALL_R2 fungi Saccharomyces 0.0 << 413.2197 407.3334 398654 398653 1 71145.279 38727448 ALL_R2 viral Phix174microvirus n/a << 1.3813 1.3635 239 1 238 238.144 23250 R1U bacteria Bacillus n/a RN7SL2(24);RN7SL1(8);COL1A2(6);GEM(6);RNR2(6) << 1.2357 1.2181 218 6 212 212.750 21527 R2U bacteria Bacillus n/a RNR2(11);RN7SL2(6);HFM1(5);UNC45A(5);RN7SK(4)