# 2020/01/22 22:12:06 # HUMAN Target (Host) organism # DNA Sequencing source # ERR018402 Replication ID # SRP001294 Sample ID # ERR018402_1.filt Read1 # ERR018402_2.filt Read2 R1 31897561 (100.0000%) [A] Total raw reads R1 26298176 (82.4457%) [B] Total reads after QC of (A) R1 R1 26298176 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 48904589 (92.9810%) [B-3] Total mapped reads of (B-2)x2 R1 3691763 (7.0190%) [B-4] Total unmapped reads of (B-2)x2 R1 24484465 (93.1033%) [F] Total mapped reads of (B-2) R1 1813711 (6.8967%) [G] Total unmapped reads of (B-2) R1 190770 (0.7254%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 26107406 (99.2746%) [J] Total mapped reads to host genome ( (B-2)-H) R1 12151 (0.0462%) [I-1] Total mapped reads of (H) to exo. genomes R1 465.423 [I-3] RPMH of (I-1) R1 462.047 [I-4] PPM of (I-1) R1 63694.501 [I-5] RPMU of (I-1) R1 68 (0.0003%) [I-6] Total chimeric reads of (I-1) R1 2.605 [I-7] RPMH of (I-6) R1 2.586 [I-8] PPM of (I-6) R1 356.450 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 31897561 (100.0000%) [A] Total raw reads R2 26298176 (82.4457%) [B] Total reads after QC of (A) R2 R2 26298176 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 48904589 (92.9810%) [B-3] Total mapped reads of (B-2)x2 R2 3691763 (7.0190%) [B-4] Total unmapped reads of (B-2)x2 R2 24420124 (92.8586%) [F] Total mapped reads of (B-2) R2 1878052 (7.1414%) [G] Total unmapped reads of (B-2) R2 199279 (0.7578%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 26098897 (99.2422%) [J] Total mapped reads to host genome ( (B-2)-H) R2 12049 (0.0458%) [I-1] Total mapped reads of (H) to exo. genomes R2 461.667 [I-3] RPMH of (I-1) R2 458.169 [I-4] PPM of (I-1) R2 60462.969 [I-5] RPMU of (I-1) R2 50 (0.0002%) [I-6] Total chimeric reads of (I-1) R2 1.916 [I-7] RPMH of (I-6) R2 1.901 [I-8] PPM of (I-6) R2 250.904 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 7.4591 7.4050 1020.7999 196 2 194 194.738 19229 ALL_R1 bacteria Bacillus 0.0 1.4381 1.4277 196.8077 59 34 25 37.545 4734 ALL_R1 bacteria Alcanivorax 1.13e-26 << 455.0050 451.7043 62268.7005 11879 0 11879 11879.000 1043642 ALL_R1 viral Lymphocryptovirus 0.0 << 7.2410 7.1861 948.3287 193 6 187 188.982 18095 ALL_R2 bacteria Streptomyces 0.0 1.2131 1.2039 158.8778 55 37 18 31.661 3692 ALL_R2 bacteria Alcanivorax 2.28e-13 << 451.1302 447.7117 59082.9942 11774 0 11774 11774.000 962449 ALL_R2 viral Lymphocryptovirus 0.0 << 2.4131 2.3956 330.2406 63 0 63 63.000 5511 R1U viral Lymphocryptovirus n/a << 1.8392 1.8252 240.8683 48 0 48 48.000 3697 R2U viral Lymphocryptovirus n/a