# 2020/11/12 14:31:36 # HUMAN Target (Host) organism # RNA Sequencing source # SRR12951220 Replication ID # SRR12951220 Sample ID # SRR12951220_1 Read1 # NULL Read2 R1 95942391 (100.0000%) [A] Total raw reads R1 95236513 (99.2643%) [B] Total reads after QC of (A) R1 R1 95236513 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 65468272 (68.7428%) [F] Total mapped reads of (B-2) R1 29768241 (31.2572%) [G] Total unmapped reads of (B-2) R1 2338785 (2.4558%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 92897728 (97.5442%) [J] Total mapped reads to host genome ( (B-2)-H) R1 756897 (0.7948%) [I-1] Total mapped reads of (H) to exo. genomes R1 8147.637 [I-3] RPMH of (I-1) R1 7947.550 [I-4] PPM of (I-1) R1 323628.294 [I-5] RPMU of (I-1) R1 1 (0.0000%) [I-6] Total chimeric reads of (I-1) R1 0.011 [I-7] RPMH of (I-6) R1 0.011 [I-8] PPM of (I-6) R1 0.428 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2426.5571 2366.9666 96384.0823 541904 539967 1937 225421.646 41053841 ALL_R1 bacteria Escherichia 0.0 2388.1753 2329.5273 94859.5339 537455 537377 78 221856.055 40717206 ALL_R1 bacteria Shigella 5.25e-60 695.6742 678.5900 27632.5314 71706 30760 40946 64626.550 5435024 ALL_R1 bacteria Akkermansia 0.0 593.5735 578.9968 23577.0428 203703 194294 9409 55141.634 15437311 ALL_R1 bacteria Acinetobacter 0.0 542.2487 528.9324 21538.3941 231498 230075 1423 50373.673 17541642 ALL_R1 bacteria Haemophilus 0.0 395.4108 385.7005 15705.9187 139827 138712 1115 36732.767 10592240 ALL_R1 bacteria Lactobacillus 0.0 166.2733 162.1900 6604.4596 122192 121825 367 15446.411 9260257 ALL_R1 bacteria Tolypothrix 3.04e-08 95.5410 93.1948 3794.9388 39310 38490 820 8875.546 2979341 ALL_R1 bacteria Exiguobacterium 0.0 38.3765 37.4341 1524.3338 40274 39868 406 3565.089 3047652 ALL_R1 bacteria Streptococcus 1.45e-08 37.8171 36.8884 1502.1150 48008 47611 397 3513.124 3639169 ALL_R1 bacteria Deinococcus 1.38e-05 17.3394 16.9136 688.7307 18537 18331 206 1610.793 1402764 ALL_R1 bacteria Anaerococcus 6.69e-05 << 314.0595 306.3470 12474.6041 44483 43564 919 29175.417 3370788 ALL_R1 fungi Candida 0.0 88.9188 86.7352 3531.9014 16728 16172 556 8260.358 1267529 ALL_R1 fungi Glarea 5.03e-35 87.3720 85.2263 3470.4597 13118 12502 616 8116.659 994530 ALL_R1 fungi Mitosporidium 1.95e-44 71.0906 69.3448 2823.7546 12199 11735 464 6604.155 923280 ALL_R1 fungi Malassezia 1.21e-26 67.6421 65.9810 2686.7780 10993 10017 976 6283.796 832195 ALL_R1 fungi Saitoella 2.32e-252 << 7.0831 6.9091 281.3427 658 0 658 658.000 49893 ALL_R1 viral Betacoronavirus 5.02e-128 4.1874 4.0846 166.3257 389 0 389 389.000 29515 ALL_R1 viral Lentivirus 4.24e-05