# 2020/01/21 17:52:35 # HUMAN Target (Host) organism # DNA Sequencing source # ERR016180 Replication ID # SRP001294 Sample ID # ERR016180_1.filt Read1 # ERR016180_2.filt Read2 R1 30396317 (100.0000%) [A] Total raw reads R1 24688346 (81.2215%) [B] Total reads after QC of (A) R1 R1 24688346 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 44784297 (90.6993%) [B-3] Total mapped reads of (B-2)x2 R1 4592395 (9.3007%) [B-4] Total unmapped reads of (B-2)x2 R1 22373500 (90.6237%) [F] Total mapped reads of (B-2) R1 2314846 (9.3763%) [G] Total unmapped reads of (B-2) R1 90841 (0.3680%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 24597505 (99.6320%) [J] Total mapped reads to host genome ( (B-2)-H) R1 5794 (0.0235%) [I-1] Total mapped reads of (H) to exo. genomes R1 235.552 [I-3] RPMH of (I-1) R1 234.686 [I-4] PPM of (I-1) R1 63781.773 [I-5] RPMU of (I-1) R1 85 (0.0003%) [I-6] Total chimeric reads of (I-1) R1 3.456 [I-7] RPMH of (I-6) R1 3.443 [I-8] PPM of (I-6) R1 935.701 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 30396317 (100.0000%) [A] Total raw reads R2 24688346 (81.2215%) [B] Total reads after QC of (A) R2 R2 24688346 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 44784297 (90.6993%) [B-3] Total mapped reads of (B-2)x2 R2 4592395 (9.3007%) [B-4] Total unmapped reads of (B-2)x2 R2 22410797 (90.7748%) [F] Total mapped reads of (B-2) R2 2277549 (9.2252%) [G] Total unmapped reads of (B-2) R2 99688 (0.4038%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 24588658 (99.5962%) [J] Total mapped reads to host genome ( (B-2)-H) R2 5692 (0.0231%) [I-1] Total mapped reads of (H) to exo. genomes R2 231.489 [I-3] RPMH of (I-1) R2 230.554 [I-4] PPM of (I-1) R2 57098.146 [I-5] RPMU of (I-1) R2 68 (0.0003%) [I-6] Total chimeric reads of (I-1) R2 2.765 [I-7] RPMH of (I-6) R2 2.754 [I-8] PPM of (I-6) R2 682.128 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 6.3867 6.3632 1729.3513 300 232 68 157.096 13457 ALL_R1 bacteria Eubacterium 8.76e-244 5.7983 5.7770 1570.0400 296 249 47 142.624 13273 ALL_R1 bacteria Alcanivorax 1.36e-155 0.7724 0.7696 209.1567 19 0 19 19.000 855 ALL_R1 bacteria Helicobacter 1.01e-103 0.7126 0.7100 192.9635 28 17 11 17.529 1256 ALL_R1 bacteria Tetrasphaera 3.52e-07 << 2.3986 2.3898 649.4865 59 0 59 59.000 2645 ALL_R1 fungi Malassezia 1.43e-289 << 216.2414 215.4458 58552.8561 5319 0 5319 5319.000 237754 ALL_R1 viral Lymphocryptovirus 0.0 << 5.2818 5.2605 1302.7947 254 201 53 129.873 11369 ALL_R2 bacteria Eubacterium 2.92e-152 5.1958 5.1748 1281.5685 263 219 44 127.757 11775 ALL_R2 bacteria Alcanivorax 1.44e-140 0.8134 0.8101 200.6260 20 0 20 20.000 900 ALL_R2 bacteria Helicobacter 6.46e-121 0.6460 0.6434 159.3371 27 18 9 15.884 1214 ALL_R2 bacteria Tetrasphaera 5.89e-05 << 1.3014 1.2962 321.0015 32 0 32 32.000 1431 ALL_R2 fungi Malassezia 2.84e-83 << 215.2212 214.3521 53085.6272 5292 0 5292 5292.000 236096 ALL_R2 viral Lymphocryptovirus 0.0 << 1.1094 1.1053 300.4040 47 32 15 27.289 2105 R1U bacteria Eubacterium n/a 0.6622 0.6598 179.3133 36 32 4 16.289 1615 R1U bacteria Alcanivorax n/a 0.0407 0.0405 11.0082 1 0 1 1.000 45 R1U bacteria Reyranella n/a << 0.4065 0.4050 110.0825 10 0 10 10.000 450 R1U fungi Malassezia n/a << 0.9351 0.9316 253.1896 23 0 23 23.000 1029 R1U viral Lymphocryptovirus n/a << 0.7549 0.7518 186.1909 34 25 9 18.561 1529 R2U bacteria Eubacterium n/a 0.5264 0.5243 129.8451 29 26 3 12.944 1304 R2U bacteria Alcanivorax n/a << 0.3254 0.3240 80.2504 8 0 8 8.000 352 R2U fungi Malassezia n/a 0.0813 0.0810 20.0626 2 0 2 2.000 90 R2U fungi Mitosporidium n/a << 0.8134 0.8101 200.6260 20 0 20 20.000 895 R2U viral Lymphocryptovirus n/a