# 2020/02/28 16:34:51 # HUMAN Target (Host) organism # DNA Sequencing source # ERR016173 Replication ID # SRP001294 Sample ID # ERR016173_1.filt Read1 # ERR016173_2.filt Read2 R1 27487567 (100.0000%) [A] Total raw reads R1 22998375 (83.6683%) [B] Total reads after QC of (A) R1 R1 22998375 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 41660651 (90.5730%) [B-3] Total mapped reads of (B-2)x2 R1 4336099 (9.4270%) [B-4] Total unmapped reads of (B-2)x2 R1 20478434 (89.0430%) [F] Total mapped reads of (B-2) R1 2519941 (10.9570%) [G] Total unmapped reads of (B-2) R1 311125 (1.3528%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 22687250 (98.6472%) [J] Total mapped reads to host genome ( (B-2)-H) R1 5008 (0.0218%) [I-1] Total mapped reads of (H) to exo. genomes R1 220.741 [I-3] RPMH of (I-1) R1 217.755 [I-4] PPM of (I-1) R1 16096.424 [I-5] RPMU of (I-1) R1 30 (0.0001%) [I-6] Total chimeric reads of (I-1) R1 1.322 [I-7] RPMH of (I-6) R1 1.304 [I-8] PPM of (I-6) R1 96.424 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 27487567 (100.0000%) [A] Total raw reads R2 22998375 (83.6683%) [B] Total reads after QC of (A) R2 R2 22998375 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 41660651 (90.5730%) [B-3] Total mapped reads of (B-2)x2 R2 4336099 (9.4270%) [B-4] Total unmapped reads of (B-2)x2 R2 21182217 (92.1031%) [F] Total mapped reads of (B-2) R2 1816158 (7.8969%) [G] Total unmapped reads of (B-2) R2 141250 (0.6142%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 22857125 (99.3858%) [J] Total mapped reads to host genome ( (B-2)-H) R2 5219 (0.0227%) [I-1] Total mapped reads of (H) to exo. genomes R2 228.331 [I-3] RPMH of (I-1) R2 226.929 [I-4] PPM of (I-1) R2 36948.673 [I-5] RPMU of (I-1) R2 28 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 1.225 [I-7] RPMH of (I-6) R2 1.217 [I-8] PPM of (I-6) R2 198.230 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 3.8170 3.7654 278.3351 228 204 24 86.597 18465 ALL_R1 bacteria Escherichia 2.56e-120 1.0260 1.0121 74.8124 41 20 21 23.276 3320 ALL_R1 bacteria Bacteroides 3.01e-19 0.7934 0.7827 57.8546 18 0 18 18.000 1458 ALL_R1 bacteria Helicobacter 3.92e-127 << 206.4155 203.6231 15051.8280 4683 0 4683 4683.000 378920 ALL_R1 viral Lymphocryptovirus 0.0 << 4.3218 4.2952 699.3487 262 234 28 98.783 17105 ALL_R2 bacteria Escherichia 4.24e-153 1.0884 1.0817 176.1204 43 20 23 24.877 2821 ALL_R2 bacteria Bacteroides 2.51e-21 0.9188 0.9131 148.6726 21 0 21 21.000 1394 ALL_R2 bacteria Helicobacter 5.88e-163 0.7923 0.7874 128.2124 34 24 10 18.110 2055 ALL_R2 bacteria Eubacterium 1.39e-06 << 211.6627 210.3627 34251.3274 4838 0 4838 4838.000 295392 ALL_R2 viral Lymphocryptovirus 0.0 << 0.0441 0.0435 3.2141 1 0 1 1.000 81 R1U bacteria Kurthia n/a << 0.0441 0.0435 3.2141 1 0 1 1.000 81 R1U fungi Mitosporidium n/a << 1.1901 1.1740 86.7818 27 0 27 27.000 2179 R1U viral Lymphocryptovirus n/a << 0.0438 0.0435 7.0796 1 0 1 1.000 68 R2U bacteria Tetrasphaera n/a 0.0438 0.0435 7.0796 1 0 1 1.000 68 R2U bacteria Raphidiopsis n/a << 0.0438 0.0435 7.0796 1 0 1 1.000 37 R2U fungi Laccaria n/a << 1.0938 1.0870 176.9912 25 0 25 25.000 1513 R2U viral Lymphocryptovirus n/a