# 2020/01/21 04:44:37 # HUMAN Target (Host) organism # DNA Sequencing source # ERR016172 Replication ID # SRP001294 Sample ID # ERR016172_1.filt Read1 # ERR016172_2.filt Read2 R1 32240016 (100.0000%) [A] Total raw reads R1 25502259 (79.1013%) [B] Total reads after QC of (A) R1 R1 25502259 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 46709023 (91.5782%) [B-3] Total mapped reads of (B-2)x2 R1 4295495 (8.4218%) [B-4] Total unmapped reads of (B-2)x2 R1 23372641 (91.6493%) [F] Total mapped reads of (B-2) R1 2129618 (8.3507%) [G] Total unmapped reads of (B-2) R1 86602 (0.3396%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 25415657 (99.6604%) [J] Total mapped reads to host genome ( (B-2)-H) R1 13434 (0.0527%) [I-1] Total mapped reads of (H) to exo. genomes R1 528.572 [I-3] RPMH of (I-1) R1 526.777 [I-4] PPM of (I-1) R1 155123.438 [I-5] RPMU of (I-1) R1 151 (0.0006%) [I-6] Total chimeric reads of (I-1) R1 5.941 [I-7] RPMH of (I-6) R1 5.921 [I-8] PPM of (I-6) R1 1743.609 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 32240016 (100.0000%) [A] Total raw reads R2 25502259 (79.1013%) [B] Total reads after QC of (A) R2 R2 25502259 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 46709023 (91.5782%) [B-3] Total mapped reads of (B-2)x2 R2 4295495 (8.4218%) [B-4] Total unmapped reads of (B-2)x2 R2 23336382 (91.5071%) [F] Total mapped reads of (B-2) R2 2165877 (8.4929%) [G] Total unmapped reads of (B-2) R2 88079 (0.3454%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 25414180 (99.6546%) [J] Total mapped reads to host genome ( (B-2)-H) R2 13406 (0.0526%) [I-1] Total mapped reads of (H) to exo. genomes R2 527.501 [I-3] RPMH of (I-1) R2 525.679 [I-4] PPM of (I-1) R2 152204.271 [I-5] RPMU of (I-1) R2 130 (0.0005%) [I-6] Total chimeric reads of (I-1) R2 5.115 [I-7] RPMH of (I-6) R2 5.098 [I-8] PPM of (I-6) R2 1475.948 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 5.2762 5.2583 1548.4515 283 238 45 134.099 12603 ALL_R1 bacteria Alcanivorax 2.79e-67 4.8249 4.8085 1415.9835 244 194 50 122.627 10900 ALL_R1 bacteria Eubacterium 8.67e-26 1.1596 1.1557 340.3155 57 44 13 29.472 2523 ALL_R1 bacteria Tetrasphaera 2.04e-04 << 0.6295 0.6274 184.7532 16 0 16 16.000 713 ALL_R1 fungi Malassezia 1.59e-07 << 514.1712 512.4252 150897.2079 13068 0 13068 13068.000 583594 ALL_R1 viral Lymphocryptovirus 0.0 << 5.8116 5.7916 1676.8810 317 271 46 147.698 14158 ALL_R2 bacteria Alcanivorax 8.20e-71 5.3880 5.3694 1554.6498 270 213 57 136.932 12070 ALL_R2 bacteria Eubacterium 3.95e-34 1.5254 1.5201 440.1276 75 58 17 38.766 3349 ALL_R2 bacteria Tetrasphaera 2.20e-08 << 0.9444 0.9411 272.4827 24 0 24 24.000 1063 ALL_R2 fungi Malassezia 6.80e-21 << 510.9746 509.2098 147435.8247 12986 0 12986 12986.000 576309 ALL_R2 viral Lymphocryptovirus 0.0 << 0.9485 0.9453 278.3769 41 27 14 24.108 1839 R1U bacteria Eubacterium n/a 0.5157 0.5140 151.3591 30 27 3 13.108 1345 R1U bacteria Alcanivorax n/a << 0.1180 0.1176 34.6412 3 0 3 3.000 128 R1U fungi Malassezia n/a << 4.0920 4.0781 1200.8961 104 0 104 104.000 4648 R1U viral Lymphocryptovirus n/a << 0.8070 0.8042 232.8364 38 28 10 20.508 1710 R2U bacteria Eubacterium n/a 0.5709 0.5689 164.7158 32 28 4 14.508 1440 R2U bacteria Alcanivorax n/a << 0.0787 0.0784 22.7069 2 0 2 2.000 81 R2U fungi Malassezia n/a << 3.3839 3.3723 976.3962 86 0 86 86.000 3742 R2U viral Lymphocryptovirus n/a