# 2018/01/31 06:31:44 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR314LXG_2 Replication ID # ENCSR314LXG Sample ID # ENCFF002DLT Read1 # ENCFF002DLU Read2 R1 77190215 (100.0000%) [A] Total raw reads R1 76171953 (98.6808%) [B] Total reads after QC of (A) R1 R1 76171953 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 145447026 (95.4728%) [B-3] Total mapped reads of (B-2)x2 R1 6896880 (4.5272%) [B-4] Total unmapped reads of (B-2)x2 R1 72801213 (95.5748%) [F] Total mapped reads of (B-2) R1 3370740 (4.4252%) [G] Total unmapped reads of (B-2) R1 1279618 (1.6799%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 74892335 (98.3201%) [J] Total mapped reads to host genome ( (B-2)-H) R1 754058 (0.9899%) [I-1] Total mapped reads of (H) to exo. genomes R1 10068.560 [I-3] RPMH of (I-1) R1 9899.418 [I-4] PPM of (I-1) R1 266 (0.0003%) [I-5] Total chimeric reads of (I-1) R1 3.552 [I-7] RPMH of (I-5) R1 3.492 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 77190215 (100.0000%) [A] Total raw reads R2 76171953 (98.6808%) [B] Total reads after QC of (A) R2 R2 76171953 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 145447026 (95.4728%) [B-3] Total mapped reads of (B-2)x2 R2 6896880 (4.5272%) [B-4] Total unmapped reads of (B-2)x2 R2 72645813 (95.3708%) [F] Total mapped reads of (B-2) R2 3526140 (4.6292%) [G] Total unmapped reads of (B-2) R2 1316635 (1.7285%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 74855318 (98.2715%) [J] Total mapped reads to host genome ( (B-2)-H) R2 746718 (0.9803%) [I-1] Total mapped reads of (H) to exo. genomes R2 9975.484 [I-3] RPMH of (I-1) R2 9803.057 [I-4] PPM of (I-1) R2 224 (0.0003%) [I-5] Total chimeric reads of (I-1) R2 2.992 [I-7] RPMH of (I-5) R2 2.941 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2939.8059 2890.4199 608886 608461 425 220168.929 58213208 ALL_R1 bacteria Pseudomonas 1.73e-14 1508.6233 1483.2798 114189 1592 112597 112984.320 11315511 ALL_R1 bacteria Bacillus 0.0 92.1619 90.6137 9639 5225 4414 6902.221 953787 ALL_R1 bacteria Pelomonas 0.0 35.9907 35.3861 4834 3427 1407 2695.427 472510 ALL_R1 bacteria Escherichia 0.0 34.3763 33.7988 3193 1085 2108 2574.520 314665 ALL_R1 bacteria Bradyrhizobium 0.0 11.2763 11.0868 1927 1498 429 844.506 192562 ALL_R1 bacteria Cutibacterium 7.64e-13 << 2967.7770 2917.9211 613585 613584 1 222263.752 58668904 ALL_R1 viral Phix174microvirus n/a << 2816.0401 2767.3647 607744 607366 378 210795.577 55768255 ALL_R2 bacteria Pseudomonas 5.43e-07 1511.7966 1485.6651 114297 1486 112811 113166.012 11440924 ALL_R2 bacteria Bacillus 0.0 97.9533 96.2601 9775 4798 4977 7332.322 966919 ALL_R2 bacteria Pelomonas 0.0 31.3449 30.8031 2653 554 2099 2346.331 260365 ALL_R2 bacteria Bradyrhizobium 0.0 13.2865 13.0568 2039 1655 384 994.564 202781 ALL_R2 bacteria Cutibacterium 1.56e-06 << 2769.6155 2721.7426 598744 598739 5 207320.449 54919488 ALL_R2 viral Phix174microvirus n/a