# 2020/10/16 00:22:58 # HUMAN Target (Host) organism # DNA Sequencing source # ERR019895 Replication ID # SRP000540 Sample ID # ERR019895_1.filt Read1 # ERR019895_2.filt Read2 R1 25718292 (100.0000%) [A] Total raw reads R1 25121220 (97.6784%) [B] Total reads after QC of (A) R1 R1 25121220 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 44150483 (87.8749%) [B-3] Total mapped reads of (B-2)x2 R1 6091957 (12.1251%) [B-4] Total unmapped reads of (B-2)x2 R1 22529341 (89.6825%) [F] Total mapped reads of (B-2) R1 2591879 (10.3175%) [G] Total unmapped reads of (B-2) R1 598910 (2.3841%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 24522310 (97.6159%) [J] Total mapped reads to host genome ( (B-2)-H) R1 29383 (0.1170%) [I-1] Total mapped reads of (H) to exo. genomes R1 1198.215 [I-3] RPMH of (I-1) R1 1169.649 [I-4] PPM of (I-1) R1 49060.794 [I-5] RPMU of (I-1) R1 19 (0.0001%) [I-6] Total chimeric reads of (I-1) R1 0.775 [I-7] RPMH of (I-6) R1 0.756 [I-8] PPM of (I-6) R1 31.724 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 25718292 (100.0000%) [A] Total raw reads R2 25121220 (97.6784%) [B] Total reads after QC of (A) R2 R2 25121220 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 44150483 (87.8749%) [B-3] Total mapped reads of (B-2)x2 R2 6091957 (12.1251%) [B-4] Total unmapped reads of (B-2)x2 R2 21621142 (86.0672%) [F] Total mapped reads of (B-2) R2 3500078 (13.9328%) [G] Total unmapped reads of (B-2) R2 1043231 (4.1528%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 24077989 (95.8472%) [J] Total mapped reads to host genome ( (B-2)-H) R2 26295 (0.1047%) [I-1] Total mapped reads of (H) to exo. genomes R2 1092.076 [I-3] RPMH of (I-1) R2 1046.725 [I-4] PPM of (I-1) R2 25205.348 [I-5] RPMU of (I-1) R2 15 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 0.623 [I-7] RPMH of (I-6) R2 0.597 [I-8] PPM of (I-6) R2 14.378 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 40.2131 39.2543 1646.5195 1065 99 966 986.117 106954 ALL_R1 bacteria Gordonia 0.0 29.4006 28.6997 1203.8052 728 10 718 720.971 78421 ALL_R1 bacteria Roseobacter 0.0 13.3178 13.0002 545.2940 884 566 318 326.582 94407 ALL_R1 bacteria Cutibacterium 4.61e-298 7.2143 7.0423 295.3900 223 58 165 176.912 22096 ALL_R1 bacteria Nocardioides 2.52e-103 5.9825 5.8399 244.9533 186 50 136 146.705 19291 ALL_R1 bacteria Bradyrhizobium 1.27e-79 2.4745 2.4155 101.3191 98 46 52 60.681 9598 ALL_R1 bacteria Sphingomonas 2.55e-21 2.3840 2.3272 97.6140 73 17 56 58.462 7280 ALL_R1 bacteria Brevundimonas 1.88e-15 1.8311 1.7875 74.9762 55 12 43 44.904 5732 ALL_R1 bacteria Burkholderia 1.67e-14 1.5918 1.5539 65.1767 64 31 33 39.035 6790 ALL_R1 bacteria Afipia 5.46e-05 1.4697 1.4346 60.1760 589 561 28 36.040 63431 ALL_R1 bacteria Pseudomonas 6.27e-04 1.3029 1.2718 53.3452 45 14 31 31.949 4682 ALL_R1 bacteria Devosia 2.00e-07 << 5.5065 5.3752 225.4629 136 1 135 135.032 13488 ALL_R1 fungi Schizosaccharomyces 1.70e-71 << 990.8936 967.2699 40572.0392 24299 0 24299 24299.000 2580951 ALL_R1 viral Lymphocryptovirus 0.0 25.5366 24.9278 1045.5945 1333 1227 106 626.217 136924 ALL_R1 viral Mastadenovirus 7.84e-94 << 34.4080 32.9791 794.1434 893 79 814 828.475 92981 ALL_R2 bacteria Gordonia 0.0 28.8424 27.6446 665.6877 705 17 688 694.466 75874 ALL_R2 bacteria Roseobacter 0.0 11.5461 11.0666 266.4856 815 543 272 278.006 87201 ALL_R2 bacteria Cutibacterium 1.69e-271 5.0346 4.8255 116.1986 162 50 112 121.222 16894 ALL_R2 bacteria Nocardioides 2.46e-57 4.8222 4.6219 111.2975 147 41 106 116.109 15401 ALL_R2 bacteria Bradyrhizobium 4.21e-59 1.9631 1.8816 45.3083 75 37 38 47.267 7920 ALL_R2 bacteria Sphingomonas 1.99e-13 1.8756 1.7977 43.2886 57 13 44 45.160 5954 ALL_R2 bacteria Brevundimonas 2.35e-11 1.5856 1.5198 36.5969 47 10 37 38.179 4883 ALL_R2 bacteria Burkholderia 2.85e-13 1.4733 1.4122 34.0049 45 10 35 35.475 4679 ALL_R2 bacteria Devosia 5.82e-14 1.3985 1.3404 32.2776 58 31 27 33.673 6184 ALL_R2 bacteria Afipia 3.70e-04 1.3350 1.2796 30.8120 558 531 27 32.144 60005 ALL_R2 bacteria Pseudomonas 1.43e-04 << 5.4822 5.2545 126.5300 133 1 132 132.000 13138 ALL_R2 fungi Schizosaccharomyces 1.55e-86 << 897.4587 860.1891 20713.5332 21609 0 21609 21609.000 2306219 ALL_R2 viral Lymphocryptovirus 0.0 27.0335 25.9108 623.9385 1330 1193 137 650.912 140759 ALL_R2 viral Mastadenovirus 3.78e-208 << 0.0816 0.0796 3.3394 2 0 2 2.000 216 R1U bacteria Roseobacter n/a 0.0408 0.0398 1.6697 1 0 1 1.000 105 R1U bacteria Gordonia n/a << 0.5301 0.5175 21.7061 13 0 13 13.000 1389 R1U viral Lymphocryptovirus n/a << 0.0831 0.0796 1.9171 2 0 2 2.000 206 R2U bacteria Roseobacter n/a << 0.1246 0.1194 2.8757 3 0 3 3.000 323 R2U viral Lymphocryptovirus n/a