# 2020/10/03 10:05:33 # HUMAN Target (Host) organism # DNA Sequencing source # SRR068129 Replication ID # SRP000547 Sample ID # SRR068129_1.filt Read1 # SRR068129_2.filt Read2 R1 42970660 (100.0000%) [A] Total raw reads R1 33927924 (78.9560%) [B] Total reads after QC of (A) R1 R1 33927924 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 61879881 (91.1931%) [B-3] Total mapped reads of (B-2)x2 R1 5975967 (8.8069%) [B-4] Total unmapped reads of (B-2)x2 R1 31305264 (92.2699%) [F] Total mapped reads of (B-2) R1 2622660 (7.7301%) [G] Total unmapped reads of (B-2) R1 358803 (1.0575%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 33569121 (98.9425%) [J] Total mapped reads to host genome ( (B-2)-H) R1 32996 (0.0973%) [I-1] Total mapped reads of (H) to exo. genomes R1 982.927 [I-3] RPMH of (I-1) R1 972.532 [I-4] PPM of (I-1) R1 91961.327 [I-5] RPMU of (I-1) R1 210 (0.0006%) [I-6] Total chimeric reads of (I-1) R1 6.256 [I-7] RPMH of (I-6) R1 6.190 [I-8] PPM of (I-6) R1 585.279 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 42970660 (100.0000%) [A] Total raw reads R2 33927924 (78.9560%) [B] Total reads after QC of (A) R2 R2 33927924 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 61879881 (91.1931%) [B-3] Total mapped reads of (B-2)x2 R2 5975967 (8.8069%) [B-4] Total unmapped reads of (B-2)x2 R2 30574617 (90.1164%) [F] Total mapped reads of (B-2) R2 3353307 (9.8836%) [G] Total unmapped reads of (B-2) R2 443631 (1.3076%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 33484293 (98.6924%) [J] Total mapped reads to host genome ( (B-2)-H) R2 32268 (0.0951%) [I-1] Total mapped reads of (H) to exo. genomes R2 963.676 [I-3] RPMH of (I-1) R2 951.075 [I-4] PPM of (I-1) R2 72736.125 [I-5] RPMU of (I-1) R2 187 (0.0006%) [I-6] Total chimeric reads of (I-1) R2 5.585 [I-7] RPMH of (I-6) R2 5.512 [I-8] PPM of (I-6) R2 421.521 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 7.3975 7.3193 692.0984 395 233 162 248.327 21624 ALL_R1 bacteria Alcanivorax 0.0 4.3048 4.2593 402.7503 146 2 144 144.508 9985 ALL_R1 bacteria Acinetobacter 0.0 << 964.9940 954.7887 90283.5261 32394 0 32394 32394.000 1893209 ALL_R1 viral Lymphocryptovirus 0.0 << 5.2714 5.2025 397.8735 331 245 86 176.509 21253 ALL_R2 bacteria Alcanivorax 4.27e-144 4.2464 4.1909 320.5096 144 2 142 142.188 10512 ALL_R2 bacteria Acinetobacter 0.0 << 947.6085 935.2178 71523.4057 31730 0 31730 31730.000 2144418 ALL_R2 viral Lymphocryptovirus 0.0 << 0.4260 0.4215 39.8520 25 17 8 14.299 1233 R1U bacteria Alcanivorax n/a << 5.5110 5.4527 515.6033 185 0 185 185.000 10874 R1U viral Lymphocryptovirus n/a << 0.3520 0.3474 26.5649 25 21 4 11.785 1717 R2U bacteria Alcanivorax n/a 0.0299 0.0295 2.2541 1 0 1 1.000 76 R2U bacteria Acinetobacter n/a 0.0299 0.0295 2.2541 1 0 1 1.000 49 R2U bacteria Klebsiella n/a 0.0299 0.0295 2.2541 1 0 1 1.000 36 R2U bacteria Fusobacterium n/a << 0.0299 0.0295 2.2541 1 0 1 1.000 73 R2U fungi Colletotrichum n/a 0.0299 0.0295 2.2541 1 0 1 1.000 59 R2U fungi Mitosporidium n/a << 4.6589 4.5980 351.6436 156 0 156 156.000 10302 R2U viral Lymphocryptovirus n/a