# 2020/10/04 22:57:03 # HUMAN Target (Host) organism # DNA Sequencing source # SRR022657 Replication ID # SRP000542 Sample ID # SRR022657_1.filt Read1 # SRR022657_2.filt Read2 R1 8817928 (100.0000%) [A] Total raw reads R1 7841586 (88.9278%) [B] Total reads after QC of (A) R1 R1 7841586 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 14296422 (91.1577%) [B-3] Total mapped reads of (B-2)x2 R1 1386750 (8.8423%) [B-4] Total unmapped reads of (B-2)x2 R1 7152918 (91.2177%) [F] Total mapped reads of (B-2) R1 688668 (8.7823%) [G] Total unmapped reads of (B-2) R1 86117 (1.0982%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 7755469 (98.9018%) [J] Total mapped reads to host genome ( (B-2)-H) R1 9535 (0.1216%) [I-1] Total mapped reads of (H) to exo. genomes R1 1229.455 [I-3] RPMH of (I-1) R1 1215.953 [I-4] PPM of (I-1) R1 110721.460 [I-5] RPMU of (I-1) R1 51 (0.0007%) [I-6] Total chimeric reads of (I-1) R1 6.576 [I-7] RPMH of (I-6) R1 6.504 [I-8] PPM of (I-6) R1 592.218 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 8817928 (100.0000%) [A] Total raw reads R2 7841586 (88.9278%) [B] Total reads after QC of (A) R2 R2 7841586 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 14296422 (91.1577%) [B-3] Total mapped reads of (B-2)x2 R2 1386750 (8.8423%) [B-4] Total unmapped reads of (B-2)x2 R2 7143504 (91.0977%) [F] Total mapped reads of (B-2) R2 698082 (8.9023%) [G] Total unmapped reads of (B-2) R2 85020 (1.0842%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 7756566 (98.9158%) [J] Total mapped reads to host genome ( (B-2)-H) R2 9531 (0.1215%) [I-1] Total mapped reads of (H) to exo. genomes R2 1228.765 [I-3] RPMH of (I-1) R2 1215.443 [I-4] PPM of (I-1) R2 112103.035 [I-5] RPMU of (I-1) R2 45 (0.0006%) [I-6] Total chimeric reads of (I-1) R2 5.801 [I-7] RPMH of (I-6) R2 5.739 [I-8] PPM of (I-6) R2 529.287 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 3.3661 3.3292 303.1457 55 46 9 26.106 3517 ALL_R1 bacteria Alcanivorax 9.92e-05 << 1212.1769 1198.8646 109165.4377 9401 0 9401 9401.000 651157 ALL_R1 viral Lymphocryptovirus 0.0 2.2744 2.2494 204.8260 47 46 1 17.639 3455 ALL_R1 viral Phix174microvirus n/a << 5.0308 4.9763 458.9744 68 46 22 39.022 4359 ALL_R2 bacteria Alcanivorax 3.80e-29 1.1603 1.1477 105.8574 9 0 9 9.000 679 ALL_R2 bacteria Vibrio 7.55e-05 << 1209.0402 1195.9315 110303.4580 9378 0 9378 9378.000 645485 ALL_R2 viral Lymphocryptovirus 0.0 5.7203 5.6583 521.8772 45 1 44 44.370 3302 ALL_R2 viral Phix174microvirus n/a << 0.1769 0.1750 15.9318 2 1 1 1.372 152 R1U bacteria Alcanivorax n/a << 6.1892 6.1212 557.3812 48 0 48 48.000 3284 R1U viral Lymphocryptovirus n/a 0.1289 0.1275 11.6121 1 0 1 1.000 76 R1U viral Lambdavirus n/a << 0.2720 0.2691 24.8177 4 3 1 2.110 304 R2U bacteria Alcanivorax n/a 0.1289 0.1275 11.7619 1 0 1 1.000 39 R2U bacteria Brachyspira n/a << 0.1289 0.1275 11.7619 1 0 1 1.000 36 R2U fungi Bipolaris n/a << 5.0280 4.9735 458.7156 39 0 39 39.000 2593 R2U viral Lymphocryptovirus n/a