# 2020/10/04 22:10:34 # HUMAN Target (Host) organism # DNA Sequencing source # SRR022656 Replication ID # SRP000542 Sample ID # SRR022656_1.filt Read1 # SRR022656_2.filt Read2 R1 9786217 (100.0000%) [A] Total raw reads R1 8806782 (89.9917%) [B] Total reads after QC of (A) R1 R1 8806782 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 15954471 (90.5806%) [B-3] Total mapped reads of (B-2)x2 R1 1659093 (9.4194%) [B-4] Total unmapped reads of (B-2)x2 R1 7997172 (90.8070%) [F] Total mapped reads of (B-2) R1 809610 (9.1930%) [G] Total unmapped reads of (B-2) R1 104959 (1.1918%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 8701823 (98.8082%) [J] Total mapped reads to host genome ( (B-2)-H) R1 10474 (0.1189%) [I-1] Total mapped reads of (H) to exo. genomes R1 1203.656 [I-3] RPMH of (I-1) R1 1189.311 [I-4] PPM of (I-1) R1 99791.347 [I-5] RPMU of (I-1) R1 46 (0.0005%) [I-6] Total chimeric reads of (I-1) R1 5.286 [I-7] RPMH of (I-6) R1 5.223 [I-8] PPM of (I-6) R1 438.266 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 9786217 (100.0000%) [A] Total raw reads R2 8806782 (89.9917%) [B] Total reads after QC of (A) R2 R2 8806782 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 15954471 (90.5806%) [B-3] Total mapped reads of (B-2)x2 R2 1659093 (9.4194%) [B-4] Total unmapped reads of (B-2)x2 R2 7957299 (90.3542%) [F] Total mapped reads of (B-2) R2 849483 (9.6458%) [G] Total unmapped reads of (B-2) R2 117824 (1.3379%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 8688958 (98.6621%) [J] Total mapped reads to host genome ( (B-2)-H) R2 10501 (0.1192%) [I-1] Total mapped reads of (H) to exo. genomes R2 1208.545 [I-3] RPMH of (I-1) R2 1192.376 [I-4] PPM of (I-1) R2 89124.457 [I-5] RPMU of (I-1) R2 41 (0.0005%) [I-6] Total chimeric reads of (I-1) R2 4.719 [I-7] RPMH of (I-6) R2 4.656 [I-8] PPM of (I-6) R2 347.977 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 3.2345 3.1959 268.1619 47 30 17 28.146 3339 ALL_R1 bacteria Alcanivorax 5.53e-16 << 1186.1882 1172.0513 98343.1626 10322 0 10322 10322.000 750732 ALL_R1 viral Lymphocryptovirus 0.0 << 5.4385 5.3658 401.0643 85 60 25 47.255 5939 ALL_R2 bacteria Alcanivorax 1.55e-36 3.5117 3.4647 258.9710 67 58 9 30.513 4687 ALL_R2 bacteria Tetrasphaera 4.79e-05 1.6112 1.5897 118.8213 14 0 14 14.000 1039 ALL_R2 bacteria Vibrio 2.67e-12 << 1185.4126 1169.5532 87418.5225 10300 0 10300 10300.000 744845 ALL_R2 viral Lymphocryptovirus 0.0 6.4877 6.4009 478.4339 57 1 56 56.371 4240 ALL_R2 viral Phix174microvirus n/a << 0.2298 0.2271 19.0551 2 0 2 2.000 152 R1U bacteria Alcanivorax n/a << 5.0564 4.9961 419.2113 44 0 44 44.000 3206 R1U viral Lymphocryptovirus n/a << 0.2005 0.1978 14.7848 3 2 1 1.742 228 R2U bacteria Alcanivorax n/a 0.1151 0.1135 8.4872 1 0 1 1.000 36 R2U bacteria Streptomyces n/a << 4.2583 4.2013 314.0277 37 0 37 37.000 2602 R2U viral Lymphocryptovirus n/a