# 2018/01/28 06:28:41 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR109IQO_2 Replication ID # ENCSR109IQO Sample ID # ENCFF002DKF Read1 # ENCFF002DKI Read2 R1 76826446 (100.0000%) [A] Total raw reads R1 71067357 (92.5038%) [B] Total reads after QC of (A) R1 R1 71067357 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 113387836 (79.7749%) [B-3] Total mapped reads of (B-2)x2 R1 28746878 (20.2251%) [B-4] Total unmapped reads of (B-2)x2 R1 56986501 (80.1866%) [F] Total mapped reads of (B-2) R1 14080856 (19.8134%) [G] Total unmapped reads of (B-2) R1 1224237 (1.7226%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 69843120 (98.2774%) [J] Total mapped reads to host genome ( (B-2)-H) R1 336693 (0.4738%) [I-1] Total mapped reads of (H) to exo. genomes R1 4820.704 [I-3] RPMH of (I-1) R1 4737.660 [I-4] PPM of (I-1) R1 341 (0.0005%) [I-5] Total chimeric reads of (I-1) R1 4.882 [I-7] RPMH of (I-5) R1 4.798 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 76826446 (100.0000%) [A] Total raw reads R2 71067357 (92.5038%) [B] Total reads after QC of (A) R2 R2 71067357 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 113387836 (79.7749%) [B-3] Total mapped reads of (B-2)x2 R2 28746878 (20.2251%) [B-4] Total unmapped reads of (B-2)x2 R2 56401335 (79.3632%) [F] Total mapped reads of (B-2) R2 14666022 (20.6368%) [G] Total unmapped reads of (B-2) R2 1269747 (1.7867%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 69797610 (98.2133%) [J] Total mapped reads to host genome ( (B-2)-H) R2 330348 (0.4648%) [I-1] Total mapped reads of (H) to exo. genomes R2 4732.941 [I-3] RPMH of (I-1) R2 4648.379 [I-4] PPM of (I-1) R2 515 (0.0007%) [I-5] Total chimeric reads of (I-1) R2 7.378 [I-7] RPMH of (I-5) R2 7.247 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 3057.4448 3004.7759 216686 3307 213379 213541.483 21431115 ALL_R1 bacteria Bacillus 0.0 100.4243 98.6943 13161 13001 160 7013.944 1270294 ALL_R1 bacteria Escherichia 6.54e-05 << 19.9302 19.5869 1599 409 1190 1391.989 105514 ALL_R1 fungi Aspergillus 0.0 << 94.6549 93.0243 9546 9427 119 6610.993 931572 ALL_R1 viral Lambdavirus 1.41e-35 << 3108.2037 3052.6700 219229 2383 216846 216945.192 21671386 ALL_R2 bacteria Bacillus 0.0 40.6205 39.8948 6234 6070 164 2835.216 613506 ALL_R2 bacteria Escherichia 1.64e-06 << 5.7948 5.6912 566 224 342 404.461 46483 ALL_R2 fungi Aspergillus 8.79e-217 << 202.5012 198.8832 102598 102597 1 14134.102 9532947 ALL_R2 viral Phix174microvirus n/a 37.0793 36.4168 3612 3521 91 2588.045 358497 ALL_R2 viral Lambdavirus 7.46e-14 << 3.2383 3.1825 231 5 226 226.170 22575 R1U bacteria Bacillus n/a RN7SL2(8);RNR2(5);HFM1(4);RN7SL1(3);ZFPM2(2) << 5.5025 5.4042 390 6 384 384.061 37656 R2U bacteria Bacillus n/a RN7SL2(18);RNR2(15);RN7SK(11);HFM1(5);SLCO5A1(5)