# 2020/09/28 21:37:34 # HUMAN Target (Host) organism # DNA Sequencing source # ERR018451 Replication ID # SRP000808 Sample ID # ERR018451_1.filt Read1 # ERR018451_2.filt Read2 R1 32641049 (100.0000%) [A] Total raw reads R1 26850568 (82.2601%) [B] Total reads after QC of (A) R1 R1 26850568 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 49514227 (92.2033%) [B-3] Total mapped reads of (B-2)x2 R1 4186909 (7.7967%) [B-4] Total unmapped reads of (B-2)x2 R1 24703270 (92.0028%) [F] Total mapped reads of (B-2) R1 2147298 (7.9972%) [G] Total unmapped reads of (B-2) R1 178090 (0.6633%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 26672478 (99.3367%) [J] Total mapped reads to host genome ( (B-2)-H) R1 18426 (0.0686%) [I-1] Total mapped reads of (H) to exo. genomes R1 690.825 [I-3] RPMH of (I-1) R1 686.242 [I-4] PPM of (I-1) R1 103464.540 [I-5] RPMU of (I-1) R1 93 (0.0003%) [I-6] Total chimeric reads of (I-1) R1 3.487 [I-7] RPMH of (I-6) R1 3.464 [I-8] PPM of (I-6) R1 522.208 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 32641049 (100.0000%) [A] Total raw reads R2 26850568 (82.2601%) [B] Total reads after QC of (A) R2 R2 26850568 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 49514227 (92.2033%) [B-3] Total mapped reads of (B-2)x2 R2 4186909 (7.7967%) [B-4] Total unmapped reads of (B-2)x2 R2 24810957 (92.4038%) [F] Total mapped reads of (B-2) R2 2039611 (7.5962%) [G] Total unmapped reads of (B-2) R2 171379 (0.6383%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 26679189 (99.3617%) [J] Total mapped reads to host genome ( (B-2)-H) R2 18361 (0.0684%) [I-1] Total mapped reads of (H) to exo. genomes R2 688.214 [I-3] RPMH of (I-1) R2 683.822 [I-4] PPM of (I-1) R2 107136.814 [I-5] RPMU of (I-1) R2 51 (0.0002%) [I-6] Total chimeric reads of (I-1) R2 1.912 [I-7] RPMH of (I-6) R2 1.899 [I-8] PPM of (I-6) R2 297.586 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1.0103 1.0036 151.3167 44 27 17 26.948 3113 ALL_R1 bacteria Alcanivorax 3.62e-05 << 688.2750 683.7099 103082.7110 18358 0 18358 18358.000 1312449 ALL_R1 viral Lymphocryptovirus 0.0 << 686.7150 682.3319 106903.4129 18321 0 18321 18321.000 1262151 ALL_R2 viral Lymphocryptovirus 0.0 << 0.0513 0.0509 7.6815 2 1 1 1.368 131 R1U bacteria Alcanivorax n/a 0.0375 0.0372 5.6151 1 0 1 1.000 38 R1U bacteria Clostridium n/a << 0.0375 0.0372 5.6151 1 0 1 1.000 76 R1U fungi Mitosporidium n/a << 3.2993 3.2774 494.1322 88 0 88 88.000 6074 R1U viral Lymphocryptovirus n/a << 1.9116 1.8994 297.5861 51 0 51 51.000 3284 R2U viral Lymphocryptovirus n/a