# 2020/08/22 00:11:07 # HUMAN Target (Host) organism # DNA Sequencing source # SRR791477 Replication ID # SRP000547 Sample ID # SRR791477_1.filt Read1 # SRR791477_2.filt Read2 R1 9781335 (100.0000%) [A] Total raw reads R1 7703964 (78.7619%) [B] Total reads after QC of (A) R1 R1 7703964 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 13962653 (90.6199%) [B-3] Total mapped reads of (B-2)x2 R1 1445275 (9.3801%) [B-4] Total unmapped reads of (B-2)x2 R1 7047674 (91.4811%) [F] Total mapped reads of (B-2) R1 656290 (8.5189%) [G] Total unmapped reads of (B-2) R1 90665 (1.1769%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 7613299 (98.8231%) [J] Total mapped reads to host genome ( (B-2)-H) R1 10722 (0.1392%) [I-1] Total mapped reads of (H) to exo. genomes R1 1408.325 [I-3] RPMH of (I-1) R1 1391.751 [I-4] PPM of (I-1) R1 118259.527 [I-5] RPMU of (I-1) R1 67 (0.0009%) [I-6] Total chimeric reads of (I-1) R1 8.800 [I-7] RPMH of (I-6) R1 8.697 [I-8] PPM of (I-6) R1 738.984 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 9781335 (100.0000%) [A] Total raw reads R2 7703964 (78.7619%) [B] Total reads after QC of (A) R2 R2 7703964 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 13962653 (90.6199%) [B-3] Total mapped reads of (B-2)x2 R2 1445275 (9.3801%) [B-4] Total unmapped reads of (B-2)x2 R2 6914979 (89.7587%) [F] Total mapped reads of (B-2) R2 788985 (10.2413%) [G] Total unmapped reads of (B-2) R2 139185 (1.8067%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 7564779 (98.1933%) [J] Total mapped reads to host genome ( (B-2)-H) R2 10530 (0.1367%) [I-1] Total mapped reads of (H) to exo. genomes R2 1391.977 [I-3] RPMH of (I-1) R2 1366.829 [I-4] PPM of (I-1) R2 75654.704 [I-5] RPMU of (I-1) R2 59 (0.0008%) [I-6] Total chimeric reads of (I-1) R2 7.799 [I-7] RPMH of (I-6) R2 7.658 [I-8] PPM of (I-6) R2 423.896 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1.8389 1.8172 154.4146 14 0 14 14.000 1213 ALL_R1 bacteria Campylobacter 1.07e-09 << 3.8091 3.7643 319.8588 29 0 29 29.000 2712 ALL_R1 fungi Cryptococcus 3.59e-10 << 1397.1604 1380.7178 117322.0096 10637 0 10637 10637.000 985916 ALL_R1 viral Lymphocryptovirus 0.0 << 4.6468 4.5628 252.5560 61 41 20 35.152 3938 ALL_R2 bacteria Alcanivorax 1.92e-22 << 3.9657 3.8941 215.5405 30 0 30 30.000 2977 ALL_R2 fungi Cryptococcus 2.50e-11 << 1374.2635 1349.4352 74691.9567 10396 0 10396 10396.000 942182 ALL_R2 viral Lymphocryptovirus 0.0 << 0.1313 0.1298 11.0296 1 0 1 1.000 38 R1U bacteria Chryseobacterium n/a << 8.5377 8.4372 716.9249 65 0 65 65.000 5842 R1U viral Lymphocryptovirus n/a << 0.1322 0.1298 7.1847 1 0 1 1.000 44 R2U bacteria Nocardia n/a 0.1322 0.1298 7.1847 1 0 1 1.000 36 R2U bacteria Clostridium n/a << 0.1322 0.1298 7.1847 1 0 1 1.000 36 R2U fungi Trichoderma n/a << 6.8740 6.7498 373.6035 52 0 52 52.000 4752 R2U viral Lymphocryptovirus n/a