# 2020/08/29 16:45:37 # HUMAN Target (Host) organism # DNA Sequencing source # SRR741369 Replication ID # SRP000547 Sample ID # SRR741369_1.filt Read1 # SRR741369_2.filt Read2 R1 79963967 (100.0000%) [A] Total raw reads R1 77394782 (96.7871%) [B] Total reads after QC of (A) R1 R1 77394782 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 141508941 (91.4202%) [B-3] Total mapped reads of (B-2)x2 R1 13280623 (8.5798%) [B-4] Total unmapped reads of (B-2)x2 R1 70911698 (91.6234%) [F] Total mapped reads of (B-2) R1 6483084 (8.3766%) [G] Total unmapped reads of (B-2) R1 1069417 (1.3818%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 76325365 (98.6182%) [J] Total mapped reads to host genome ( (B-2)-H) R1 98015 (0.1266%) [I-1] Total mapped reads of (H) to exo. genomes R1 1284.173 [I-3] RPMH of (I-1) R1 1266.429 [I-4] PPM of (I-1) R1 91652.742 [I-5] RPMU of (I-1) R1 133 (0.0002%) [I-6] Total chimeric reads of (I-1) R1 1.743 [I-7] RPMH of (I-6) R1 1.718 [I-8] PPM of (I-6) R1 124.367 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 79963967 (100.0000%) [A] Total raw reads R2 77394782 (96.7871%) [B] Total reads after QC of (A) R2 R2 77394782 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 141508941 (91.4202%) [B-3] Total mapped reads of (B-2)x2 R2 13280623 (8.5798%) [B-4] Total unmapped reads of (B-2)x2 R2 70597243 (91.2171%) [F] Total mapped reads of (B-2) R2 6797539 (8.7829%) [G] Total unmapped reads of (B-2) R2 1203579 (1.5551%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 76191203 (98.4449%) [J] Total mapped reads to host genome ( (B-2)-H) R2 96905 (0.1252%) [I-1] Total mapped reads of (H) to exo. genomes R2 1271.866 [I-3] RPMH of (I-1) R2 1252.087 [I-4] PPM of (I-1) R2 80514.034 [I-5] RPMU of (I-1) R2 106 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 1.391 [I-7] RPMH of (I-6) R2 1.370 [I-8] PPM of (I-6) R2 88.071 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 3.5849 3.5354 255.8581 305 66 239 273.619 28436 ALL_R1 bacteria Escherichia 0.0 << 552.6996 545.0626 39446.7266 42185 0 42185 42185.000 4119001 ALL_R1 viral Lymphocryptovirus 0.0 << 3.4462 3.3926 218.1602 294 69 225 262.573 26975 ALL_R2 bacteria Escherichia 0.0 << 549.8273 541.2768 34806.1905 41892 0 41892 41892.000 4075511 ALL_R2 viral Lymphocryptovirus 0.0 << 0.4717 0.4651 33.6632 36 0 36 36.000 3080 R1U viral Lymphocryptovirus n/a << 0.2917 0.2872 18.4658 55 54 1 22.225 3571 R2U bacteria Acinetobacter n/a 0.0295 0.0291 1.8686 3 2 1 2.249 192 R2U bacteria Alcanivorax n/a 0.0213 0.0210 1.3493 2 1 1 1.624 101 R2U bacteria Tetrasphaera n/a << 0.4725 0.4651 29.9108 36 0 36 36.000 2195 R2U viral Lymphocryptovirus n/a