# 2020/08/09 12:46:04 # HUMAN Target (Host) organism # DNA Sequencing source # SRR741362 Replication ID # SRP001294 Sample ID # SRR741362_1.filt Read1 # SRR741362_2.filt Read2 R1 65764352 (100.0000%) [A] Total raw reads R1 64351925 (97.8523%) [B] Total reads after QC of (A) R1 R1 64351925 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 117469354 (91.2710%) [B-3] Total mapped reads of (B-2)x2 R1 11234496 (8.7290%) [B-4] Total unmapped reads of (B-2)x2 R1 58869483 (91.4805%) [F] Total mapped reads of (B-2) R1 5482442 (8.5195%) [G] Total unmapped reads of (B-2) R1 1155078 (1.7949%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 63196847 (98.2051%) [J] Total mapped reads to host genome ( (B-2)-H) R1 110445 (0.1716%) [I-1] Total mapped reads of (H) to exo. genomes R1 1747.635 [I-3] RPMH of (I-1) R1 1716.266 [I-4] PPM of (I-1) R1 95616.919 [I-5] RPMU of (I-1) R1 54 (0.0001%) [I-6] Total chimeric reads of (I-1) R1 0.855 [I-7] RPMH of (I-6) R1 0.839 [I-8] PPM of (I-6) R1 46.750 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 65764352 (100.0000%) [A] Total raw reads R2 64351925 (97.8523%) [B] Total reads after QC of (A) R2 R2 64351925 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 117469354 (91.2710%) [B-3] Total mapped reads of (B-2)x2 R2 11234496 (8.7290%) [B-4] Total unmapped reads of (B-2)x2 R2 58599871 (91.0616%) [F] Total mapped reads of (B-2) R2 5752054 (8.9384%) [G] Total unmapped reads of (B-2) R2 1241191 (1.9288%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 63110734 (98.0712%) [J] Total mapped reads to host genome ( (B-2)-H) R2 109701 (0.1705%) [I-1] Total mapped reads of (H) to exo. genomes R2 1738.231 [I-3] RPMH of (I-1) R2 1704.704 [I-4] PPM of (I-1) R2 88383.657 [I-5] RPMU of (I-1) R2 37 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 0.586 [I-7] RPMH of (I-6) R2 0.575 [I-8] PPM of (I-6) R2 29.810 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 5.2836 5.1888 289.0783 343 21 322 333.908 33454 ALL_R1 bacteria Escherichia 0.0 << 427.3314 419.6611 23380.2393 27006 0 27006 27006.000 2664980 ALL_R1 viral Lymphocryptovirus 0.0 << 5.1781 5.0782 263.2882 336 25 311 326.791 31329 ALL_R2 bacteria Escherichia 0.0 << 510.0368 500.1995 25933.7999 81087 81086 1 32188.799 7725749 ALL_R2 viral Phix174microvirus n/a 426.3300 418.1072 21677.5661 26906 0 26906 26906.000 2623825 ALL_R2 viral Lymphocryptovirus 0.0 << 0.1108 0.1088 6.0602 7 0 7 7.000 705 R1U viral Lymphocryptovirus n/a << 0.0475 0.0466 2.4170 3 0 3 3.000 207 R2U viral Lymphocryptovirus n/a