# 2020/08/07 08:29:34 # HUMAN Target (Host) organism # DNA Sequencing source # SRR035600 Replication ID # SRP001294 Sample ID # SRR035600_1.filt Read1 # SRR035600_2.filt Read2 R1 13818936 (100.0000%) [A] Total raw reads R1 11925637 (86.2992%) [B] Total reads after QC of (A) R1 R1 11925637 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 20255831 (84.9256%) [B-3] Total mapped reads of (B-2)x2 R1 3595443 (15.0744%) [B-4] Total unmapped reads of (B-2)x2 R1 10153488 (85.1400%) [F] Total mapped reads of (B-2) R1 1772149 (14.8600%) [G] Total unmapped reads of (B-2) R1 1063095 (8.9144%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 10862542 (91.0856%) [J] Total mapped reads to host genome ( (B-2)-H) R1 8268 (0.0693%) [I-1] Total mapped reads of (H) to exo. genomes R1 761.148 [I-3] RPMH of (I-1) R1 693.296 [I-4] PPM of (I-1) R1 7777.292 [I-5] RPMU of (I-1) R1 116 (0.0010%) [I-6] Total chimeric reads of (I-1) R1 10.679 [I-7] RPMH of (I-6) R1 9.727 [I-8] PPM of (I-6) R1 109.115 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 13818936 (100.0000%) [A] Total raw reads R2 11925637 (86.2992%) [B] Total reads after QC of (A) R2 R2 11925637 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 20255831 (84.9256%) [B-3] Total mapped reads of (B-2)x2 R2 3595443 (15.0744%) [B-4] Total unmapped reads of (B-2)x2 R2 10102343 (84.7111%) [F] Total mapped reads of (B-2) R2 1823294 (15.2889%) [G] Total unmapped reads of (B-2) R2 1062864 (8.9124%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 10862773 (91.0876%) [J] Total mapped reads to host genome ( (B-2)-H) R2 8075 (0.0677%) [I-1] Total mapped reads of (H) to exo. genomes R2 743.364 [I-3] RPMH of (I-1) R2 677.113 [I-4] PPM of (I-1) R2 7597.397 [I-5] RPMU of (I-1) R2 76 (0.0006%) [I-6] Total chimeric reads of (I-1) R2 6.996 [I-7] RPMH of (I-6) R2 6.373 [I-8] PPM of (I-6) R2 71.505 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 0.8624 0.7855 8.8120 10 1 9 9.368 626 ALL_R1 bacteria Alcanivorax 3.27e-06 << 759.4907 691.7869 7760.3601 8250 0 8250 8250.000 586635 ALL_R1 viral Lymphocryptovirus 0.0 << 1.2888 1.1739 13.1720 14 0 14 14.000 778 ALL_R2 bacteria Alcanivorax 5.34e-16 << 741.7075 675.6033 7580.4618 8057 0 8057 8057.000 544918 ALL_R2 viral Lymphocryptovirus 0.0 << 10.5868 9.6431 108.1747 115 0 115 115.000 8270 R1U viral Lymphocryptovirus n/a << 6.8123 6.2051 69.6232 74 0 74 74.000 5106 R2U viral Lymphocryptovirus n/a