# 2018/02/10 01:34:44 # HUMAN Target (Host) organism # DNA Sequencing source # H1703_3 Replication ID # H1703 Sample ID # DRR016865_1 Read1 # DRR016865_2 Read2 R1 199143378 (100.0000%) [A] Total raw reads R1 187014123 (93.9093%) [B] Total reads after QC of (A) R1 R1 187014123 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 319753049 (85.4890%) [B-3] Total mapped reads of (B-2)x2 R1 54275197 (14.5110%) [B-4] Total unmapped reads of (B-2)x2 R1 162396040 (86.8362%) [F] Total mapped reads of (B-2) R1 24618083 (13.1638%) [G] Total unmapped reads of (B-2) R1 9441454 (5.0485%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 177572669 (94.9515%) [J] Total mapped reads to host genome ( (B-2)-H) R1 5499 (0.0029%) [I-1] Total mapped reads of (H) to exo. genomes R1 30.968 [I-3] RPMH of (I-1) R1 29.404 [I-4] PPM of (I-1) R1 122 (0.0001%) [I-5] Total chimeric reads of (I-1) R1 0.687 [I-7] RPMH of (I-5) R1 0.652 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 199143378 (100.0000%) [A] Total raw reads R2 187014123 (93.9093%) [B] Total reads after QC of (A) R2 R2 187014123 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 319753049 (85.4890%) [B-3] Total mapped reads of (B-2)x2 R2 54275197 (14.5110%) [B-4] Total unmapped reads of (B-2)x2 R2 157357009 (84.1418%) [F] Total mapped reads of (B-2) R2 29657114 (15.8582%) [G] Total unmapped reads of (B-2) R2 7052099 (3.7709%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 179962024 (96.2291%) [J] Total mapped reads to host genome ( (B-2)-H) R2 3966 (0.0021%) [I-1] Total mapped reads of (H) to exo. genomes R2 22.038 [I-3] RPMH of (I-1) R2 21.207 [I-4] PPM of (I-1) R2 53 (0.0000%) [I-5] Total chimeric reads of (I-1) R2 0.294 [I-7] RPMH of (I-5) R2 0.283 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 5.3021 5.0344 960 30 930 941.501 81030 ALL_R1 bacteria Mesorhizobium 0.0 3.8576 3.6629 695 13 682 685.005 69847 ALL_R1 bacteria Cutibacterium 0.0 2.9657 2.8160 1135 813 322 526.627 113732 ALL_R1 bacteria Campylobacter 0.0 2.2073 2.0959 571 389 182 391.955 52911 ALL_R1 bacteria Alcanivorax 0.0 1.5677 1.4885 327 73 254 278.374 32621 ALL_R1 bacteria Burkholderia 0.0 1.3704 1.3012 850 823 27 243.350 83605 ALL_R1 bacteria Eubacterium 3.10e-38 1.2277 1.1658 242 29 213 218.013 24103 ALL_R1 bacteria Pseudomonas 0.0 1.1745 1.1152 352 311 41 208.560 33602 ALL_R1 bacteria Tetrasphaera 3.34e-125 << 3.6070 3.4710 667 27 640 649.125 66908 ALL_R2 bacteria Cutibacterium 0.0 3.1938 3.0733 1097 784 313 574.758 110043 ALL_R2 bacteria Campylobacter 0.0 1.7107 1.6462 341 41 300 307.864 34044 ALL_R2 bacteria Burkholderia 0.0 1.4282 1.3743 751 735 16 257.017 75158 ALL_R2 bacteria Eubacterium 6.52e-26 1.2712 1.2233 329 225 104 228.767 31643 ALL_R2 bacteria Alcanivorax 0.0 1.2156 1.1698 240 24 216 218.770 23943 ALL_R2 bacteria Pseudomonas 0.0