# 2020/08/06 04:38:03 # HUMAN Target (Host) organism # DNA Sequencing source # ERR018523 Replication ID # SRP001294 Sample ID # ERR018523_1.filt Read1 # ERR018523_2.filt Read2 R1 4531202 (100.0000%) [A] Total raw reads R1 4427201 (97.7048%) [B] Total reads after QC of (A) R1 R1 4427201 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 7692723 (86.8802%) [B-3] Total mapped reads of (B-2)x2 R1 1161679 (13.1198%) [B-4] Total unmapped reads of (B-2)x2 R1 3862848 (87.2526%) [F] Total mapped reads of (B-2) R1 564353 (12.7474%) [G] Total unmapped reads of (B-2) R1 128333 (2.8987%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 4298868 (97.1013%) [J] Total mapped reads to host genome ( (B-2)-H) R1 330 (0.0075%) [I-1] Total mapped reads of (H) to exo. genomes R1 76.764 [I-3] RPMH of (I-1) R1 74.539 [I-4] PPM of (I-1) R1 2571.435 [I-5] RPMU of (I-1) R1 1 (0.0000%) [I-6] Total chimeric reads of (I-1) R1 0.233 [I-7] RPMH of (I-6) R1 0.226 [I-8] PPM of (I-6) R1 7.792 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 4531202 (100.0000%) [A] Total raw reads R2 4427201 (97.7048%) [B] Total reads after QC of (A) R2 R2 4427201 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 7692723 (86.8802%) [B-3] Total mapped reads of (B-2)x2 R2 1161679 (13.1198%) [B-4] Total unmapped reads of (B-2)x2 R2 3829875 (86.5078%) [F] Total mapped reads of (B-2) R2 597326 (13.4922%) [G] Total unmapped reads of (B-2) R2 163319 (3.6890%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 4263882 (96.3110%) [J] Total mapped reads to host genome ( (B-2)-H) R2 339 (0.0077%) [I-1] Total mapped reads of (H) to exo. genomes R2 79.505 [I-3] RPMH of (I-1) R2 76.572 [I-4] PPM of (I-1) R2 2075.692 [I-5] RPMU of (I-1) R2 4 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 0.938 [I-7] RPMH of (I-6) R2 0.903 [I-8] PPM of (I-6) R2 24.492 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1.2720 1.2351 42.6079 14 13 1 5.468 1422 ALL_R1 bacteria Salmonella 1.76e-07 0.4652 0.4518 15.5845 2 0 2 2.000 197 ALL_R1 bacteria Streptococcus 7.01e-18 0.2326 0.2259 7.7922 1 0 1 1.000 108 ALL_R1 bacteria Clostridioides 9.56e-12 << 68.3901 66.4076 2290.9150 294 0 294 294.000 31328 ALL_R1 viral Lymphocryptovirus 0.0 1.9045 1.8492 63.7950 16 13 3 8.187 1630 ALL_R1 viral Mastadenovirus 7.37e-64 << 3.7009 3.5643 96.6207 28 20 8 15.780 2938 ALL_R2 bacteria Streptomyces 0.0 0.6515 0.6275 17.0097 4 2 2 2.778 424 ALL_R2 bacteria Streptococcus 5.36e-17 0.2345 0.2259 6.1230 1 0 1 1.000 44 ALL_R2 bacteria Psychroflexus 2.06e-08 0.2345 0.2259 6.1230 1 0 1 1.000 108 ALL_R2 bacteria Clostridioides 3.47e-11 0.2345 0.2259 6.1230 1 0 1 1.000 107 ALL_R2 bacteria Alcanivorax 3.95e-07 << 0.2345 0.2259 6.1230 1 0 1 1.000 106 ALL_R2 fungi Mitosporidium 2.06e-08 << 69.6548 67.0853 1818.5269 297 0 297 297.000 31728 ALL_R2 viral Lymphocryptovirus 0.0 2.3978 2.3094 62.6014 20 16 4 10.224 2006 ALL_R2 viral Mastadenovirus 2.76e-108 0.7036 0.6776 18.3690 3 0 3 3.000 211 ALL_R2 viral Lambdavirus 6.67e-23 << 0.2326 0.2259 7.7922 1 0 1 1.000 107 R1U viral Lymphocryptovirus n/a << 0.2345 0.2259 6.1230 1 0 1 1.000 106 R2U fungi Mitosporidium n/a