# 2020/08/15 03:47:29 # HUMAN Target (Host) organism # DNA Sequencing source # ERR016261 Replication ID # SRP000808 Sample ID # ERR016261_1.filt Read1 # ERR016261_2.filt Read2 R1 38147910 (100.0000%) [A] Total raw reads R1 30670043 (80.3977%) [B] Total reads after QC of (A) R1 R1 30670043 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 56654511 (92.3613%) [B-3] Total mapped reads of (B-2)x2 R1 4685575 (7.6387%) [B-4] Total unmapped reads of (B-2)x2 R1 28356813 (92.4577%) [F] Total mapped reads of (B-2) R1 2313230 (7.5423%) [G] Total unmapped reads of (B-2) R1 214553 (0.6996%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 30455490 (99.3004%) [J] Total mapped reads to host genome ( (B-2)-H) R1 17592 (0.0574%) [I-1] Total mapped reads of (H) to exo. genomes R1 577.630 [I-3] RPMH of (I-1) R1 573.589 [I-4] PPM of (I-1) R1 81993.727 [I-5] RPMU of (I-1) R1 174 (0.0006%) [I-6] Total chimeric reads of (I-1) R1 5.713 [I-7] RPMH of (I-6) R1 5.673 [I-8] PPM of (I-6) R1 810.988 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 38147910 (100.0000%) [A] Total raw reads R2 30670043 (80.3977%) [B] Total reads after QC of (A) R2 R2 30670043 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 56654511 (92.3613%) [B-3] Total mapped reads of (B-2)x2 R2 4685575 (7.6387%) [B-4] Total unmapped reads of (B-2)x2 R2 28297698 (92.2649%) [F] Total mapped reads of (B-2) R2 2372345 (7.7351%) [G] Total unmapped reads of (B-2) R2 223948 (0.7302%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 30446095 (99.2698%) [J] Total mapped reads to host genome ( (B-2)-H) R2 17432 (0.0568%) [I-1] Total mapped reads of (H) to exo. genomes R2 572.553 [I-3] RPMH of (I-1) R2 568.372 [I-4] PPM of (I-1) R2 77839.498 [I-5] RPMU of (I-1) R2 123 (0.0004%) [I-6] Total chimeric reads of (I-1) R2 4.040 [I-7] RPMH of (I-6) R2 4.010 [I-8] PPM of (I-6) R2 549.235 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 575.8896 571.8610 81746.7013 17539 0 17539 17539.000 1508418 ALL_R1 viral Lymphocryptovirus 0.0 << 1.2035 1.1947 163.6139 60 37 23 36.641 3973 ALL_R2 bacteria Alcanivorax 3.03e-10 << 570.0567 565.8942 77500.1340 17356 0 17356 17356.000 1456632 ALL_R2 viral Lymphocryptovirus 0.0 << 0.0692 0.0687 9.8158 4 3 1 2.106 316 R1U bacteria Alcanivorax n/a << 5.5819 5.5429 792.3450 170 0 170 170.000 14400 R1U viral Lymphocryptovirus n/a << 0.1107 0.1098 15.0437 4 1 3 3.369 240 R2U bacteria Alcanivorax n/a << 3.8757 3.8474 526.9080 118 0 118 118.000 9759 R2U viral Lymphocryptovirus n/a