# 2020/05/28 07:14:35 # HUMAN Target (Host) organism # RNA Sequencing source # WIV04_1 Replication ID # WIV04 Sample ID # SRR11092057_1 Read1 # SRR11092057_2 Read2 R1 5184909 (100.0000%) [A] Total raw reads R1 5055154 (97.4974%) [B] Total reads after QC of (A) R1 R1 5055154 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 8766278 (86.7063%) [B-3] Total mapped reads of (B-2)x2 R1 1344030 (13.2937%) [B-4] Total unmapped reads of (B-2)x2 R1 4415682 (87.3501%) [F] Total mapped reads of (B-2) R1 639472 (12.6499%) [G] Total unmapped reads of (B-2) R1 129993 (2.5715%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 4925161 (97.4285%) [J] Total mapped reads to host genome ( (B-2)-H) R1 11225 (0.2221%) [I-1] Total mapped reads of (H) to exo. genomes R1 2279.113 [I-3] RPMH of (I-1) R1 2220.506 [I-4] PPM of (I-1) R1 86350.803 [I-5] RPMU of (I-1) R1 1 (0.0000%) [I-6] Total chimeric reads of (I-1) R1 0.203 [I-7] RPMH of (I-6) R1 0.198 [I-8] PPM of (I-6) R1 7.693 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 5184909 (100.0000%) [A] Total raw reads R2 5055154 (97.4974%) [B] Total reads after QC of (A) R2 R2 5055154 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 8766278 (86.7063%) [B-3] Total mapped reads of (B-2)x2 R2 1344030 (13.2937%) [B-4] Total unmapped reads of (B-2)x2 R2 4350596 (86.0626%) [F] Total mapped reads of (B-2) R2 704558 (13.9374%) [G] Total unmapped reads of (B-2) R2 150721 (2.9815%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 4904433 (97.0185%) [J] Total mapped reads to host genome ( (B-2)-H) R2 11169 (0.2209%) [I-1] Total mapped reads of (H) to exo. genomes R2 2277.327 [I-3] RPMH of (I-1) R2 2209.428 [I-4] PPM of (I-1) R2 74103.808 [I-5] RPMU of (I-1) R2 3 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 0.612 [I-7] RPMH of (I-6) R2 0.593 [I-8] PPM of (I-6) R2 19.904 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 262.0479 255.3093 9928.4423 1435 428 1007 1290.628 210536 ALL_R1 bacteria Actinomyces 0.0 223.5082 217.7607 8468.2560 4634 4509 125 1100.814 692665 ALL_R1 bacteria Pseudomonas 0.0 124.7781 121.5694 4727.5776 668 61 607 614.552 95402 ALL_R1 bacteria Rothia 0.0 98.3016 95.7737 3724.4390 657 330 327 484.151 91061 ALL_R1 bacteria Veillonella 0.0 72.2671 70.4087 2738.0474 510 446 64 355.927 73469 ALL_R1 bacteria Cellulomonas 8.22e-165 66.0793 64.3800 2503.6040 417 214 203 325.451 56463 ALL_R1 bacteria Capnocytophaga 0.0 38.4002 37.4127 1454.9014 359 252 107 189.127 48229 ALL_R1 bacteria Streptococcus 0.0 35.3923 34.4822 1340.9414 374 359 15 174.313 50888 ALL_R1 bacteria Megasphaera 1.55e-06 25.8241 25.1601 978.4219 380 304 76 127.188 53538 ALL_R1 bacteria Neisseria 8.35e-233 25.7612 25.0987 976.0372 290 195 95 126.878 41119 ALL_R1 bacteria Pelomonas 2.73e-123 23.5537 22.9481 892.4019 325 245 80 116.006 45601 ALL_R1 bacteria Comamonas 0.0 21.1451 20.6014 801.1431 149 73 76 104.143 20738 ALL_R1 bacteria Cutibacterium 8.30e-168 17.8321 17.3736 675.6210 199 128 71 87.826 27756 ALL_R1 bacteria Cardiobacterium 2.95e-217 17.1365 16.6958 649.2657 158 110 48 84.400 21384 ALL_R1 bacteria Prevotella 2.97e-131 12.8863 12.5549 488.2340 237 200 37 63.467 33135 ALL_R1 bacteria Delftia 6.60e-288 12.0607 11.7506 456.9554 157 106 51 59.401 21900 ALL_R1 bacteria Corynebacterium 0.0 11.0390 10.7552 418.2456 250 234 16 54.369 35025 ALL_R1 bacteria Acidovorax 9.95e-24 10.8315 10.5530 410.3836 189 145 44 53.347 26103 ALL_R1 bacteria Lautropia 1.31e-67 10.5796 10.3075 400.8370 134 89 45 52.106 18642 ALL_R1 bacteria Aeromonas 6.03e-138 9.2121 8.9752 349.0265 99 89 10 45.371 12833 ALL_R1 bacteria Chryseobacterium 3.83e-04 8.6690 8.4460 328.4485 202 173 29 42.696 28064 ALL_R1 bacteria Ralstonia 8.89e-20 7.1192 6.9361 269.7299 68 33 35 35.063 8666 ALL_R1 bacteria Thermus 1.23e-39 6.5669 6.3980 248.8057 113 84 29 32.343 15286 ALL_R1 bacteria Moraxella 4.94e-39 5.6753 5.5294 215.0270 184 166 18 27.952 25289 ALL_R1 bacteria Ottowia 6.34e-12 4.5493 4.4323 172.3631 63 42 21 22.406 8516 ALL_R1 bacteria Bradyrhizobium 1.01e-08 3.8742 3.7746 146.7848 78 63 15 19.081 10680 ALL_R1 bacteria Rhodococcus 1.68e-05 3.2279 3.1449 122.2989 33 18 15 15.898 4541 ALL_R1 bacteria Campylobacter 3.41e-10 2.7863 2.7147 105.5672 62 49 13 13.723 8307 ALL_R1 bacteria Methylobacterium 7.04e-06 2.5510 2.4854 96.6514 52 40 12 12.564 6662 ALL_R1 bacteria Deinococcus 1.91e-06 << 338.2631 329.5646 12816.0747 1666 0 1666 1666.000 242550 ALL_R1 viral Betacoronavirus 0.0 << 248.4691 241.0609 8085.1374 1369 429 940 1218.600 200261 ALL_R2 bacteria Actinomyces 0.0 246.5771 239.2254 8023.5734 4713 4578 135 1209.321 702826 ALL_R2 bacteria Pseudomonas 0.0 125.4065 121.6675 4080.7054 671 65 606 615.048 95620 ALL_R2 bacteria Rothia 0.0 95.4074 92.5628 3104.5375 652 351 301 467.919 90208 ALL_R2 bacteria Veillonella 0.0 66.2558 64.2803 2155.9504 486 434 52 324.947 70209 ALL_R2 bacteria Cellulomonas 8.01e-107 64.2480 62.3324 2090.6178 416 220 196 315.100 56202 ALL_R2 bacteria Capnocytophaga 0.0 37.3823 36.2677 1216.4131 399 385 14 183.339 54364 ALL_R2 bacteria Megasphaera 1.18e-05 35.6720 34.6084 1160.7606 340 228 112 174.951 45374 ALL_R2 bacteria Streptococcus 0.0 27.2221 26.4105 885.8022 402 333 69 133.509 56733 ALL_R2 bacteria Neisseria 1.99e-191 24.6289 23.8946 801.4212 339 251 88 120.791 47480 ALL_R2 bacteria Comamonas 0.0 22.9812 22.2961 747.8055 149 37 112 112.710 20683 ALL_R2 bacteria Cutibacterium 0.0 22.3221 21.6565 726.3553 263 177 86 109.477 36994 ALL_R2 bacteria Pelomonas 1.38e-101 18.3159 17.7698 595.9952 195 125 70 89.829 27329 ALL_R2 bacteria Cardiobacterium 4.12e-213 17.7299 17.2013 576.9269 160 109 51 86.955 21573 ALL_R2 bacteria Prevotella 9.46e-151 13.2313 12.8368 430.5439 228 188 40 64.892 31843 ALL_R2 bacteria Delftia 0.0 12.3258 11.9583 401.0788 159 107 52 60.451 22033 ALL_R2 bacteria Corynebacterium 0.0 10.9425 10.6163 356.0685 196 152 44 53.667 27308 ALL_R2 bacteria Lautropia 2.41e-68 10.6691 10.3510 347.1713 134 89 45 52.326 18617 ALL_R2 bacteria Aeromonas 1.86e-139 10.5688 10.2537 343.9070 243 227 16 51.834 33875 ALL_R2 bacteria Acidovorax 5.39e-24 8.2797 8.0328 269.4183 90 80 10 40.607 11544 ALL_R2 bacteria Chryseobacterium 3.45e-04 8.2301 7.9847 267.8061 199 170 29 40.364 27729 ALL_R2 bacteria Ralstonia 5.53e-20 7.1435 6.9306 232.4494 60 25 35 35.035 7530 ALL_R2 bacteria Thermus 4.56e-40 6.4305 6.2388 209.2476 112 83 29 31.538 15002 ALL_R2 bacteria Moraxella 1.83e-39 6.0588 5.8782 197.1524 177 160 17 29.715 24277 ALL_R2 bacteria Ottowia 1.33e-10 3.9525 3.8347 128.6151 82 66 16 19.385 11189 ALL_R2 bacteria Rhodococcus 2.35e-06 3.4334 3.3311 111.7230 53 37 16 16.839 7055 ALL_R2 bacteria Bradyrhizobium 5.78e-05 3.3180 3.2191 107.9677 40 25 15 16.273 5429 ALL_R2 bacteria Campylobacter 2.64e-10 2.9471 2.8593 95.8990 66 53 13 14.454 8914 ALL_R2 bacteria Methylobacterium 6.09e-06 2.4417 2.3689 79.4514 53 42 11 11.975 6828 ALL_R2 bacteria Deinococcus 2.18e-05 << 337.8576 327.7843 10993.8230 1657 0 1657 1657.000 241105 ALL_R2 viral Betacoronavirus 0.0 << 0.2030 0.1978 7.6927 1 0 1 1.000 151 R1U bacteria Actinomyces n/a << 0.2298 0.2229 7.4774 2 1 1 1.127 302 R2U bacteria Rothia n/a