# 2020/05/31 22:34:37 # HUMAN Target (Host) organism # RNA Sequencing source # WHU01_1 Replication ID # WHU01 Sample ID # SRR10903402_1 Read1 # SRR10903402_2 Read2 R1 676694 (100.0000%) [A] Total raw reads R1 450652 (66.5961%) [B] Total reads after QC of (A) R1 R1 450652 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 36345 (4.0325%) [B-3] Total mapped reads of (B-2)x2 R1 864959 (95.9675%) [B-4] Total unmapped reads of (B-2)x2 R1 26988 (5.9887%) [F] Total mapped reads of (B-2) R1 423664 (94.0113%) [G] Total unmapped reads of (B-2) R1 406212 (90.1387%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 44440 (9.8613%) [J] Total mapped reads to host genome ( (B-2)-H) R1 383325 (85.0601%) [I-1] Total mapped reads of (H) to exo. genomes R1 8625675.068 [I-3] RPMH of (I-1) R1 850600.907 [I-4] PPM of (I-1) R1 943657.499 [I-5] RPMU of (I-1) R1 35 (0.0078%) [I-6] Total chimeric reads of (I-1) R1 787.579 [I-7] RPMH of (I-6) R1 77.665 [I-8] PPM of (I-6) R1 86.162 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 676694 (100.0000%) [A] Total raw reads R2 450652 (66.5961%) [B] Total reads after QC of (A) R2 R2 450652 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 36345 (4.0325%) [B-3] Total mapped reads of (B-2)x2 R2 864959 (95.9675%) [B-4] Total unmapped reads of (B-2)x2 R2 9357 (2.0763%) [F] Total mapped reads of (B-2) R2 441295 (97.9237%) [G] Total unmapped reads of (B-2) R2 433977 (96.2998%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 16675 (3.7002%) [J] Total mapped reads to host genome ( (B-2)-H) R2 384575 (85.3375%) [I-1] Total mapped reads of (H) to exo. genomes R2 23062968.516 [I-3] RPMH of (I-1) R2 853374.666 [I-4] PPM of (I-1) R2 886164.474 [I-5] RPMU of (I-1) R2 216 (0.0479%) [I-6] Total chimeric reads of (I-1) R2 12953.523 [I-7] RPMH of (I-6) R2 479.305 [I-8] PPM of (I-6) R2 497.722 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 113169.2169 11159.9194 12380.8258 5233 489 4744 5029.240 787380 ALL_R1 bacteria Capnocytophaga 0.0 38508.6184 3797.4379 4212.8815 2425 1267 1158 1711.323 364543 ALL_R1 bacteria Streptococcus 0.0 32883.3933 3242.7194 3597.4762 1694 452 1242 1461.338 254630 ALL_R1 bacteria Prevotella 0.0 32089.1764 3164.3996 3510.5881 1578 328 1250 1426.043 237357 ALL_R1 bacteria Veillonella 0.0 16724.4374 1649.2415 1829.6702 1457 1118 339 743.234 219043 ALL_R1 bacteria Lactobacillus 9.45e-109 12518.2493 1234.4581 1369.5090 1055 744 311 556.311 158720 ALL_R1 bacteria Lautropia 2.70e-229 12071.1296 1190.3664 1320.5937 677 174 503 536.441 101672 ALL_R1 bacteria Mycoplasma 1.61e-165 8041.2916 792.9733 879.7254 510 224 286 357.355 76461 ALL_R1 bacteria Escherichia 8.30e-44 << 948064.8065 93491.2083 103719.2402 42132 0 42132 42132.000 6340424 ALL_R1 viral Betacoronavirus 0.0 << 297260.0300 10999.1989 11421.8288 5173 465 4708 4956.811 778340 ALL_R2 bacteria Capnocytophaga 0.0 94597.6012 3500.2951 3634.7894 2377 1305 1072 1577.415 357490 ALL_R2 bacteria Streptococcus 0.0 84465.5472 3125.3894 3245.4784 1666 454 1212 1408.463 250661 ALL_R2 bacteria Prevotella 0.0 82742.8486 3061.6462 3179.2860 1550 335 1215 1379.737 233153 ALL_R2 bacteria Veillonella 0.0 41038.9805 1518.5221 1576.8693 1474 1150 324 684.325 221704 ALL_R2 bacteria Lactobacillus 5.58e-90 34188.8456 1265.0537 1313.6618 1054 688 366 570.099 158514 ALL_R2 bacteria Lautropia 0.0 31035.2624 1148.3650 1192.4895 669 190 479 517.513 100530 ALL_R2 bacteria Mycoplasma 6.55e-142 20290.5547 750.7900 779.6381 505 231 274 338.345 75892 ALL_R2 bacteria Escherichia 3.18e-36 << 9296232.0840 343978.6576 357195.5887 322570 322569 1 155014.670 48400730 ALL_R2 viral Phix174microvirus n/a 2503748.1259 92643.5476 96203.2550 41750 0 41750 41750.000 6283977 ALL_R2 viral Betacoronavirus 0.0 << 106.9982 10.5514 11.7057 5 1 4 4.755 751 R1U bacteria Capnocytophaga n/a 66.1791 6.5261 7.2401 4 3 1 2.941 602 R1U bacteria Lactobacillus n/a 22.5023 2.2190 2.4618 1 0 1 1.000 151 R1U bacteria Veillonella n/a << 472.5473 46.5991 51.6971 21 0 21 21.000 3155 R1U viral Betacoronavirus n/a << 299.8501 11.0950 11.5213 5 0 5 5.000 749 R2U bacteria Capnocytophaga n/a 119.9400 4.4380 4.6085 2 0 2 2.000 302 R2U bacteria Prevotella n/a 119.9400 4.4380 4.6085 2 0 2 2.000 301 R2U bacteria Veillonella n/a 108.9655 4.0319 4.1869 3 2 1 1.817 446 R2U bacteria Neisseria n/a 59.9700 2.2190 2.3043 1 0 1 1.000 149 R2U bacteria Lactobacillus n/a << 2098.9505 77.6652 80.6494 35 0 35 35.000 5249 R2U viral Betacoronavirus n/a