# 2018/01/19 12:48:47 # HUMAN Target (Host) organism # RNA Sequencing source # L-428_1 Replication ID # L-428 Sample ID # CVCL_1361_1 Read1 # CVCL_1361_2 Read2 R1 75199596 (100.0000%) [A] Total raw reads R1 69233147 (92.0658%) [B] Total reads after QC of (A) R1 R1 69233147 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 131096139 (94.6773%) [B-3] Total mapped reads of (B-2)x2 R1 7370155 (5.3227%) [B-4] Total unmapped reads of (B-2)x2 R1 65632975 (94.7999%) [F] Total mapped reads of (B-2) R1 3600172 (5.2001%) [G] Total unmapped reads of (B-2) R1 75820 (0.1095%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 69157327 (99.8905%) [J] Total mapped reads to host genome ( (B-2)-H) R1 3614 (0.0052%) [I-1] Total mapped reads of (H) to exo. genomes R1 52.258 [I-3] RPMH of (I-1) R1 52.200 [I-4] PPM of (I-1) R1 33 (0.0000%) [I-5] Total chimeric reads of (I-1) R1 0.477 [I-7] RPMH of (I-5) R1 0.477 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 75199596 (100.0000%) [A] Total raw reads R2 69233147 (92.0658%) [B] Total reads after QC of (A) R2 R2 69233147 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 131096139 (94.6773%) [B-3] Total mapped reads of (B-2)x2 R2 7370155 (5.3227%) [B-4] Total unmapped reads of (B-2)x2 R2 65463164 (94.5547%) [F] Total mapped reads of (B-2) R2 3769983 (5.4453%) [G] Total unmapped reads of (B-2) R2 88257 (0.1275%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 69144890 (99.8725%) [J] Total mapped reads to host genome ( (B-2)-H) R2 3668 (0.0053%) [I-1] Total mapped reads of (H) to exo. genomes R2 53.048 [I-3] RPMH of (I-1) R2 52.980 [I-4] PPM of (I-1) R2 48 (0.0001%) [I-5] Total chimeric reads of (I-1) R2 0.694 [I-7] RPMH of (I-5) R2 0.693 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 6.0905 6.0838 756 549 207 421.203 73836 ALL_R1 bacteria Pseudomonas 0.0 4.1033 4.0988 746 694 52 283.771 73174 ALL_R1 bacteria Sphingomonas 0.0 2.7751 2.7720 413 317 96 191.917 40658 ALL_R1 bacteria Streptococcus 0.0 2.5984 2.5955 401 288 113 179.696 39312 ALL_R1 bacteria Cutibacterium 0.0 2.5864 2.5835 468 412 56 178.866 45691 ALL_R1 bacteria Sphingobium 0.0 2.0617 2.0595 596 592 4 142.583 58401 ALL_R1 bacteria Novosphingobium 4.00e-04 1.8307 1.8287 416 407 9 126.605 40787 ALL_R1 bacteria Lactobacillus 1.20e-16 1.7530 1.7511 397 354 43 121.236 38959 ALL_R1 bacteria Clostridium 1.10e-210 1.7484 1.7464 372 333 39 120.912 36627 ALL_R1 bacteria Acinetobacter 5.86e-145 1.4672 1.4656 356 333 23 101.467 34795 ALL_R1 bacteria Enterobacter 0.0 1.3412 1.3397 157 92 65 92.751 15184 ALL_R1 bacteria Mucilaginibacter 0.0 1.3073 1.3059 353 347 6 90.412 34425 ALL_R1 bacteria Escherichia 9.16e-18 1.1424 1.1412 317 300 17 79.008 31016 ALL_R1 bacteria Agrobacterium 8.98e-69 1.1037 1.1025 299 268 31 76.331 29199 ALL_R1 bacteria Neisseria 0.0 1.0877 1.0865 389 371 18 75.225 38114 ALL_R1 bacteria Corynebacterium 2.03e-152 1.0266 1.0255 350 343 7 70.998 34319 ALL_R1 bacteria Nakamurella 2.75e-12 << 1.4355 1.4339 318 269 49 99.273 31393 ALL_R1 fungi Malassezia 0.0 << 1.2571 1.2557 88 9 79 86.937 8508 ALL_R1 viral Gammaretrovirus 0.0 << 6.1633 6.1555 748 536 212 426.164 72751 ALL_R2 bacteria Pseudomonas 0.0 4.2510 4.2455 742 695 47 293.932 72416 ALL_R2 bacteria Sphingomonas 0.0 2.7994 2.7959 493 438 55 193.567 48195 ALL_R2 bacteria Sphingobium 0.0 2.6448 2.6414 413 324 89 182.872 40379 ALL_R2 bacteria Streptococcus 0.0 2.6229 2.6196 371 244 127 181.362 36151 ALL_R2 bacteria Cutibacterium 0.0 2.3226 2.3196 600 594 6 160.595 58672 ALL_R2 bacteria Novosphingobium 2.29e-09 1.8032 1.8009 440 433 7 124.681 42998 ALL_R2 bacteria Lactobacillus 1.43e-09 1.7914 1.7891 414 376 38 123.864 40609 ALL_R2 bacteria Clostridium 3.64e-158 1.7783 1.7760 370 347 23 122.957 35972 ALL_R2 bacteria Enterobacter 0.0 1.7611 1.7588 378 340 38 121.770 36828 ALL_R2 bacteria Acinetobacter 2.46e-133 1.4086 1.4068 411 407 4 97.397 39824 ALL_R2 bacteria Burkholderia 3.52e-05 1.2499 1.2483 152 131 21 86.423 14764 ALL_R2 bacteria Mucilaginibacter 1.24e-61 1.2000 1.1985 388 376 12 82.974 37919 ALL_R2 bacteria Corynebacterium 1.51e-61 1.1667 1.1652 294 268 26 80.669 28862 ALL_R2 bacteria Agrobacterium 7.89e-160 1.1360 1.1346 316 288 28 78.549 30836 ALL_R2 bacteria Neisseria 0.0 1.0046 1.0033 303 291 12 69.460 29624 ALL_R2 bacteria Stenotrophomonas 3.83e-23 << 1.2469 1.2454 317 276 41 86.220 31002 ALL_R2 fungi Malassezia 0.0 << 1.0763 1.0749 75 4 71 74.421 7180 ALL_R2 viral Gammaretrovirus 0.0