# 2018/01/17 18:36:26 # HUMAN Target (Host) organism # RNA Sequencing source # BL-41_1 Replication ID # BL-41 Sample ID # CVCL_1087_1 Read1 # CVCL_1087_2 Read2 R1 100704028 (100.0000%) [A] Total raw reads R1 76228823 (75.6959%) [B] Total reads after QC of (A) R1 R1 76228823 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 143831415 (94.3419%) [B-3] Total mapped reads of (B-2)x2 R1 8626231 (5.6581%) [B-4] Total unmapped reads of (B-2)x2 R1 72392351 (94.9672%) [F] Total mapped reads of (B-2) R1 3836472 (5.0328%) [G] Total unmapped reads of (B-2) R1 212427 (0.2787%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 76016396 (99.7213%) [J] Total mapped reads to host genome ( (B-2)-H) R1 4203 (0.0055%) [I-1] Total mapped reads of (H) to exo. genomes R1 55.291 [I-3] RPMH of (I-1) R1 55.137 [I-4] PPM of (I-1) R1 95 (0.0001%) [I-5] Total chimeric reads of (I-1) R1 1.250 [I-7] RPMH of (I-5) R1 1.246 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 100704028 (100.0000%) [A] Total raw reads R2 76228823 (75.6959%) [B] Total reads after QC of (A) R2 R2 76228823 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 143831415 (94.3419%) [B-3] Total mapped reads of (B-2)x2 R2 8626231 (5.6581%) [B-4] Total unmapped reads of (B-2)x2 R2 71439064 (93.7166%) [F] Total mapped reads of (B-2) R2 4789759 (6.2834%) [G] Total unmapped reads of (B-2) R2 546275 (0.7166%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 75682548 (99.2834%) [J] Total mapped reads to host genome ( (B-2)-H) R2 4399 (0.0058%) [I-1] Total mapped reads of (H) to exo. genomes R2 58.124 [I-3] RPMH of (I-1) R2 57.708 [I-4] PPM of (I-1) R2 260 (0.0003%) [I-5] Total chimeric reads of (I-1) R2 3.435 [I-7] RPMH of (I-5) R2 3.411 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 8.9075 8.8827 1126 702 424 677.118 104120 ALL_R1 bacteria Pseudomonas 0.0 3.3095 3.3003 829 782 47 251.577 76508 ALL_R1 bacteria Sphingomonas 7.33e-261 2.6784 2.6710 378 220 158 203.606 35195 ALL_R1 bacteria Staphylococcus 0.0 2.3887 2.3821 514 383 131 181.584 47260 ALL_R1 bacteria Corynebacterium 0.0 2.3761 2.3695 492 400 92 180.623 45342 ALL_R1 bacteria Sphingobium 0.0 2.3195 2.3130 529 463 66 176.321 49478 ALL_R1 bacteria Acinetobacter 0.0 2.2477 2.2414 401 256 145 170.862 35644 ALL_R1 bacteria Brachybacterium 0.0 1.7890 1.7840 624 615 9 135.993 58148 ALL_R1 bacteria Novosphingobium 6.10e-16 1.5936 1.5891 386 290 96 121.137 35592 ALL_R1 bacteria Brevibacterium 0.0 1.4404 1.4363 434 412 22 109.491 40180 ALL_R1 bacteria Enterobacter 2.76e-227 1.4085 1.4046 439 424 15 107.068 40683 ALL_R1 bacteria Rhizobium 2.81e-24 1.3124 1.3088 424 409 15 99.765 39455 ALL_R1 bacteria Agrobacterium 6.08e-39 1.2796 1.2760 408 392 16 97.267 37939 ALL_R1 bacteria Psychrobacter 5.91e-45 1.2669 1.2634 443 432 11 96.304 41068 ALL_R1 bacteria Moraxella 2.42e-07 1.2643 1.2608 393 348 45 96.108 36124 ALL_R1 bacteria Actinomyces 0.0 1.2029 1.1996 417 413 4 91.441 38766 ALL_R1 bacteria Citrobacter 2.85e-04 1.1043 1.1012 397 389 8 83.943 36665 ALL_R1 bacteria Stenotrophomonas 8.61e-09 1.1002 1.0971 427 414 13 83.633 39577 ALL_R1 bacteria Burkholderia 2.56e-50 1.0771 1.0741 395 366 29 81.874 36789 ALL_R1 bacteria Methylobacterium 4.13e-79 1.0100 1.0072 424 416 8 76.775 39420 ALL_R1 bacteria Aeromonas 7.94e-12 << 3.5170 3.5072 271 22 249 267.349 24989 ALL_R1 viral Gammaretrovirus 0.0 << 8.1236 8.0654 1110 745 365 614.813 100471 ALL_R2 bacteria Pseudomonas 0.0 3.3472 3.3232 819 772 47 253.323 73748 ALL_R2 bacteria Sphingomonas 1.57e-237 2.6939 2.6746 514 372 142 203.879 45497 ALL_R2 bacteria Corynebacterium 0.0 2.6502 2.6312 378 221 157 200.576 34498 ALL_R2 bacteria Staphylococcus 0.0 2.4115 2.3942 474 393 81 182.510 42976 ALL_R2 bacteria Sphingobium 0.0 2.3073 2.2907 404 270 134 174.621 35270 ALL_R2 bacteria Brachybacterium 0.0 2.1126 2.0974 536 473 63 159.884 49605 ALL_R2 bacteria Acinetobacter 1.54e-258 1.9760 1.9619 605 600 5 149.550 54457 ALL_R2 bacteria Novosphingobium 2.93e-04 1.7334 1.7210 383 289 94 131.186 33361 ALL_R2 bacteria Brevibacterium 0.0 1.3675 1.3577 395 355 40 103.497 34431 ALL_R2 bacteria Actinomyces 2.17e-266 1.3644 1.3547 425 411 14 103.264 38440 ALL_R2 bacteria Rhizobium 4.32e-19 1.2115 1.2029 406 392 14 91.692 36750 ALL_R2 bacteria Agrobacterium 8.98e-31 1.1926 1.1840 417 404 13 90.258 38507 ALL_R2 bacteria Psychrobacter 2.38e-26 1.1599 1.1516 388 375 13 87.785 34136 ALL_R2 bacteria Arthrobacter 4.74e-14 1.1417 1.1335 448 430 18 86.403 40566 ALL_R2 bacteria Enterobacter 2.12e-137 1.1346 1.1265 402 369 33 85.872 35955 ALL_R2 bacteria Methylobacterium 2.09e-94 1.1250 1.1170 467 456 11 85.145 41500 ALL_R2 bacteria Burkholderia 1.19e-31 1.0277 1.0203 457 452 5 77.779 41327 ALL_R2 bacteria Escherichia 1.19e-05 << 3.3350 3.3111 254 17 237 252.403 21706 ALL_R2 viral Gammaretrovirus 0.0