# 2018/01/17 23:56:23 # HUMAN Target (Host) organism # RNA Sequencing source # A3_Kawakami_1 Replication ID # A3_Kawakami Sample ID # CVCL_1062_1 Read1 # CVCL_1062_2 Read2 R1 91667724 (100.0000%) [A] Total raw reads R1 82257773 (89.7347%) [B] Total reads after QC of (A) R1 R1 82257773 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 156706940 (95.2536%) [B-3] Total mapped reads of (B-2)x2 R1 7808606 (4.7464%) [B-4] Total unmapped reads of (B-2)x2 R1 78447033 (95.3673%) [F] Total mapped reads of (B-2) R1 3810740 (4.6327%) [G] Total unmapped reads of (B-2) R1 97965 (0.1191%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 82159808 (99.8809%) [J] Total mapped reads to host genome ( (B-2)-H) R1 3576 (0.0043%) [I-1] Total mapped reads of (H) to exo. genomes R1 43.525 [I-3] RPMH of (I-1) R1 43.473 [I-4] PPM of (I-1) R1 34 (0.0000%) [I-5] Total chimeric reads of (I-1) R1 0.414 [I-7] RPMH of (I-5) R1 0.413 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 91667724 (100.0000%) [A] Total raw reads R2 82257773 (89.7347%) [B] Total reads after QC of (A) R2 R2 82257773 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 156706940 (95.2536%) [B-3] Total mapped reads of (B-2)x2 R2 7808606 (4.7464%) [B-4] Total unmapped reads of (B-2)x2 R2 78259907 (95.1398%) [F] Total mapped reads of (B-2) R2 3997866 (4.8602%) [G] Total unmapped reads of (B-2) R2 132165 (0.1607%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 82125608 (99.8393%) [J] Total mapped reads to host genome ( (B-2)-H) R2 3613 (0.0044%) [I-1] Total mapped reads of (H) to exo. genomes R2 43.994 [I-3] RPMH of (I-1) R2 43.923 [I-4] PPM of (I-1) R2 66 (0.0001%) [I-5] Total chimeric reads of (I-1) R2 0.804 [I-7] RPMH of (I-5) R2 0.802 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 7.0034 6.9951 1072 717 355 575.399 103424 ALL_R1 bacteria Neisseria 0.0 5.1992 5.1930 866 636 230 427.162 83127 ALL_R1 bacteria Pseudomonas 0.0 5.0153 5.0094 693 460 233 412.060 66085 ALL_R1 bacteria Cutibacterium 0.0 2.0710 2.0686 635 600 35 170.156 60774 ALL_R1 bacteria Sphingomonas 2.59e-198 1.9502 1.9479 641 637 4 160.228 61494 ALL_R1 bacteria Burkholderia 1.83e-05 1.5683 1.5664 447 397 50 128.850 42733 ALL_R1 bacteria Acinetobacter 3.46e-246 1.3555 1.3538 507 477 30 111.364 48192 ALL_R1 bacteria Acidovorax 2.91e-118 1.3396 1.3380 297 239 58 110.063 28355 ALL_R1 bacteria Sphingobium 0.0 1.2580 1.2565 477 473 4 103.355 45620 ALL_R1 bacteria Ralstonia 1.01e-09 1.2122 1.2107 635 631 4 99.593 60374 ALL_R1 bacteria Comamonas 6.31e-04 1.1541 1.1527 431 407 24 94.822 41104 ALL_R1 bacteria Corynebacterium 6.30e-246 1.0940 1.0927 406 393 13 89.883 38630 ALL_R1 bacteria Enterobacter 7.59e-108 << 6.3927 6.3824 1073 896 177 525.006 103417 ALL_R2 bacteria Neisseria 0.0 5.1918 5.1834 882 647 235 426.378 84887 ALL_R2 bacteria Pseudomonas 0.0 5.1155 5.1073 685 381 304 420.117 65177 ALL_R2 bacteria Cutibacterium 0.0 2.2494 2.2458 671 638 33 184.736 64080 ALL_R2 bacteria Sphingomonas 5.70e-172 1.8998 1.8967 668 662 6 156.019 64258 ALL_R2 bacteria Burkholderia 7.36e-13 1.6247 1.6220 467 412 55 133.426 45316 ALL_R2 bacteria Acinetobacter 8.01e-293 1.5342 1.5317 342 284 58 125.994 32588 ALL_R2 bacteria Sphingobium 0.0 1.3480 1.3458 526 523 3 110.704 50647 ALL_R2 bacteria Ralstonia 4.15e-05 1.3273 1.3252 522 493 29 109.004 50315 ALL_R2 bacteria Acidovorax 7.23e-108 1.2285 1.2265 443 420 23 100.893 42084 ALL_R2 bacteria Corynebacterium 5.38e-220 1.0613 1.0596 416 400 16 87.163 39927 ALL_R2 bacteria Enterobacter 1.61e-161