# 2020/06/05 13:41:07 # HUMAN Target (Host) organism # RNA Sequencing source # ERR188411 Replication ID # ERR188411 Sample ID # ERR188411_1 Read1 # ERR188411_2 Read2 R1 26967190 (100.0000%) [A] Total raw reads R1 26452140 (98.0901%) [B] Total reads after QC of (A) R1 R1 26452140 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 50211899 (94.9108%) [B-3] Total mapped reads of (B-2)x2 R1 2692381 (5.0892%) [B-4] Total unmapped reads of (B-2)x2 R1 25138045 (95.0322%) [F] Total mapped reads of (B-2) R1 1314095 (4.9678%) [G] Total unmapped reads of (B-2) R1 58637 (0.2217%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 26393503 (99.7783%) [J] Total mapped reads to host genome ( (B-2)-H) R1 38549 (0.1457%) [I-1] Total mapped reads of (H) to exo. genomes R1 1460.549 [I-3] RPMH of (I-1) R1 1457.311 [I-4] PPM of (I-1) R1 657417.671 [I-5] RPMU of (I-1) R1 87 (0.0003%) [I-6] Total chimeric reads of (I-1) R1 3.296 [I-7] RPMH of (I-6) R1 3.289 [I-8] PPM of (I-6) R1 1483.705 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 26967190 (100.0000%) [A] Total raw reads R2 26452140 (98.0901%) [B] Total reads after QC of (A) R2 R2 26452140 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 50211899 (94.9108%) [B-3] Total mapped reads of (B-2)x2 R2 2692381 (5.0892%) [B-4] Total unmapped reads of (B-2)x2 R2 25073854 (94.7895%) [F] Total mapped reads of (B-2) R2 1378286 (5.2105%) [G] Total unmapped reads of (B-2) R2 81703 (0.3089%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 26370437 (99.6911%) [J] Total mapped reads to host genome ( (B-2)-H) R2 38281 (0.1447%) [I-1] Total mapped reads of (H) to exo. genomes R2 1451.663 [I-3] RPMH of (I-1) R2 1447.180 [I-4] PPM of (I-1) R2 468538.487 [I-5] RPMU of (I-1) R2 96 (0.0004%) [I-6] Total chimeric reads of (I-1) R2 3.640 [I-7] RPMH of (I-6) R2 3.629 [I-8] PPM of (I-6) R2 1174.988 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 131.8950 131.6026 59368.1634 4103 1125 2978 3481.171 306265 ALL_R1 bacteria Nocardia 0.0 2.7695 2.7634 1246.6190 160 93 67 73.098 11854 ALL_R1 bacteria Cutibacterium 2.83e-38 1.6161 1.6125 727.4247 92 60 32 42.654 6818 ALL_R1 bacteria Escherichia 9.33e-05 1.2569 1.2541 565.7349 128 98 30 33.173 9478 ALL_R1 bacteria Micrococcus 5.11e-07 << 9.4895 9.4685 4271.3986 270 23 247 250.462 19650 ALL_R1 fungi Saccharomyces 0.0 << 1050.7510 1048.4218 472960.7586 27733 0 27733 27733.000 2053276 ALL_R1 viral Lymphocryptovirus 0.0 << 121.0038 120.6300 39055.1387 4085 1670 2415 3190.922 302749 ALL_R2 bacteria Nocardia 0.0 2.6780 2.6697 864.3501 164 98 66 70.620 12062 ALL_R2 bacteria Cutibacterium 1.62e-37 << 9.5835 9.5539 3093.1790 268 19 249 252.722 19619 ALL_R2 fungi Saccharomyces 0.0 << 1045.1856 1041.9573 337343.7940 27562 0 27562 27562.000 2041212 ALL_R2 viral Lymphocryptovirus 0.0 0.6447 0.6427 208.0707 17 0 17 17.000 1275 ALL_R2 viral Lambdavirus 3.87e-12 << 0.3769 0.3761 169.6540 12 4 8 9.948 897 R1U bacteria Nocardia n/a 0.0379 0.0378 17.0541 1 0 1 1.000 75 R1U bacteria Methylobacterium n/a << 0.0379 0.0378 17.0541 1 0 1 1.000 48 R1U fungi Auricularia n/a << 2.4248 2.4195 1091.4610 64 0 64 64.000 4717 R1U viral Lymphocryptovirus n/a << 0.0940 0.0937 30.3294 4 3 1 2.478 300 R2U bacteria Nocardia n/a 0.0379 0.0378 12.2395 1 0 1 1.000 70 R2U bacteria Desulfocurvus n/a 0.0379 0.0378 12.2395 1 0 1 1.000 41 R2U bacteria Desulfovibrio n/a 0.0379 0.0378 12.2395 1 0 1 1.000 37 R2U bacteria Comamonas n/a 0.0379 0.0378 12.2395 1 0 1 1.000 36 R2U bacteria Frankia n/a << 0.0658 0.0656 21.2477 3 2 1 1.736 148 R2U fungi Trichoderma n/a 0.0379 0.0378 12.2395 2 1 1 1.000 139 R2U fungi Dichomitus n/a 0.0379 0.0378 12.2395 2 1 1 1.000 119 R2U fungi Cryptococcus n/a 0.0379 0.0378 12.2395 2 1 1 1.000 113 R2U fungi Metarhizium n/a 0.0379 0.0378 12.2395 2 1 1 1.000 108 R2U fungi Malassezia n/a 0.0379 0.0378 12.2395 2 1 1 1.000 105 R2U fungi Phycomyces n/a 0.0379 0.0378 12.2395 2 1 1 1.000 104 R2U fungi Fomitiporia n/a 0.0379 0.0378 12.2395 2 1 1 1.000 102 R2U fungi Pneumocystis n/a << 2.6545 2.6463 856.7617 70 0 70 70.000 5153 R2U viral Lymphocryptovirus n/a