# 2020/05/24 21:01:03 # HUMAN Target (Host) organism # DNA Sequencing source # SRR043411 Replication ID # SRP001294 Sample ID # SRR043411_1.filt Read1 # SRR043411_2.filt Read2 R1 22880374 (100.0000%) [A] Total raw reads R1 20478424 (89.5021%) [B] Total reads after QC of (A) R1 R1 20478424 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 36120812 (88.1924%) [B-3] Total mapped reads of (B-2)x2 R1 4836036 (11.8076%) [B-4] Total unmapped reads of (B-2)x2 R1 18071680 (88.2474%) [F] Total mapped reads of (B-2) R1 2406744 (11.7526%) [G] Total unmapped reads of (B-2) R1 1232719 (6.0196%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 19245705 (93.9804%) [J] Total mapped reads to host genome ( (B-2)-H) R1 18119 (0.0885%) [I-1] Total mapped reads of (H) to exo. genomes R1 941.457 [I-3] RPMH of (I-1) R1 884.785 [I-4] PPM of (I-1) R1 14698.402 [I-5] RPMU of (I-1) R1 160 (0.0008%) [I-6] Total chimeric reads of (I-1) R1 8.314 [I-7] RPMH of (I-6) R1 7.813 [I-8] PPM of (I-6) R1 129.794 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 22880374 (100.0000%) [A] Total raw reads R2 20478424 (89.5021%) [B] Total reads after QC of (A) R2 R2 20478424 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 36120812 (88.1924%) [B-3] Total mapped reads of (B-2)x2 R2 4836036 (11.8076%) [B-4] Total unmapped reads of (B-2)x2 R2 18049132 (88.1373%) [F] Total mapped reads of (B-2) R2 2429292 (11.8627%) [G] Total unmapped reads of (B-2) R2 1232624 (6.0191%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 19245800 (93.9809%) [J] Total mapped reads to host genome ( (B-2)-H) R2 17933 (0.0876%) [I-1] Total mapped reads of (H) to exo. genomes R2 931.788 [I-3] RPMH of (I-1) R2 875.702 [I-4] PPM of (I-1) R2 14548.638 [I-5] RPMU of (I-1) R2 81 (0.0004%) [I-6] Total chimeric reads of (I-1) R2 4.209 [I-7] RPMH of (I-6) R2 3.955 [I-8] PPM of (I-6) R2 65.714 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2.7800 2.6127 43.4032 55 2 53 53.504 3682 ALL_R1 bacteria Bradyrhizobium 3.41e-30 << 935.1697 878.8762 14600.2455 17998 0 17998 17998.000 1262499 ALL_R1 viral Lymphocryptovirus 0.0 << 2.7048 2.5420 42.2327 59 11 48 52.057 3942 ALL_R2 bacteria Bradyrhizobium 8.72e-25 << 925.6565 869.9400 14452.9070 17815 0 17815 17815.000 1255003 ALL_R2 viral Lymphocryptovirus 0.0 << 8.3135 7.8131 129.7944 160 0 160 160.000 11099 R1U viral Lymphocryptovirus n/a << 4.2087 3.9554 65.7135 81 0 81 81.000 5764 R2U viral Lymphocryptovirus n/a