# 2020/05/30 15:27:10 # HUMAN Target (Host) organism # DNA Sequencing source # ERR016259 Replication ID # SRP000808 Sample ID # ERR016259_1.filt Read1 # ERR016259_2.filt Read2 R1 35976148 (100.0000%) [A] Total raw reads R1 28445643 (79.0681%) [B] Total reads after QC of (A) R1 R1 28445643 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 51762311 (90.9846%) [B-3] Total mapped reads of (B-2)x2 R1 5128975 (9.0154%) [B-4] Total unmapped reads of (B-2)x2 R1 25926032 (91.1424%) [F] Total mapped reads of (B-2) R1 2519611 (8.8576%) [G] Total unmapped reads of (B-2) R1 257148 (0.9040%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 28188495 (99.0960%) [J] Total mapped reads to host genome ( (B-2)-H) R1 16386 (0.0576%) [I-1] Total mapped reads of (H) to exo. genomes R1 581.301 [I-3] RPMH of (I-1) R1 576.046 [I-4] PPM of (I-1) R1 63722.059 [I-5] RPMU of (I-1) R1 133 (0.0005%) [I-6] Total chimeric reads of (I-1) R1 4.718 [I-7] RPMH of (I-6) R1 4.676 [I-8] PPM of (I-6) R1 517.212 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 35976148 (100.0000%) [A] Total raw reads R2 28445643 (79.0681%) [B] Total reads after QC of (A) R2 R2 28445643 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 51762311 (90.9846%) [B-3] Total mapped reads of (B-2)x2 R2 5128975 (9.0154%) [B-4] Total unmapped reads of (B-2)x2 R2 25836279 (90.8268%) [F] Total mapped reads of (B-2) R2 2609364 (9.1732%) [G] Total unmapped reads of (B-2) R2 299848 (1.0541%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 28145795 (98.9459%) [J] Total mapped reads to host genome ( (B-2)-H) R2 16123 (0.0567%) [I-1] Total mapped reads of (H) to exo. genomes R2 572.839 [I-3] RPMH of (I-1) R2 566.800 [I-4] PPM of (I-1) R2 53770.577 [I-5] RPMU of (I-1) R2 99 (0.0003%) [I-6] Total chimeric reads of (I-1) R2 3.517 [I-7] RPMH of (I-6) R2 3.480 [I-8] PPM of (I-6) R2 330.167 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 4.2617 4.2231 467.1629 223 164 59 120.130 15529 ALL_R1 bacteria Alcanivorax 1.95e-78 2.5156 2.4929 275.7634 165 150 15 70.912 11811 ALL_R1 bacteria Tetrasphaera 5.70e-06 2.0549 2.0363 225.2555 87 43 44 57.924 5910 ALL_R1 bacteria Escherichia 2.14e-28 << 568.7781 563.6364 62349.3086 16033 0 16033 16033.000 1107764 ALL_R1 viral Lymphocryptovirus 0.0 << 4.5021 4.4547 422.6008 237 176 61 126.716 16231 ALL_R2 bacteria Alcanivorax 4.08e-87 2.7394 2.7105 257.1369 183 169 14 77.102 12718 ALL_R2 bacteria Tetrasphaera 3.23e-05 2.1044 2.0823 197.5368 94 49 45 59.231 6404 ALL_R2 bacteria Escherichia 7.06e-31 << 559.9060 554.0040 52556.6287 15759 0 15759 15759.000 1051308 ALL_R2 viral Lymphocryptovirus 0.0 << 0.5573 0.5522 61.0893 27 18 9 15.709 1935 R1U bacteria Alcanivorax n/a 0.3090 0.3062 33.8677 20 18 2 8.709 1456 R1U bacteria Tetrasphaera n/a << 0.0355 0.0352 3.8888 1 0 1 1.000 47 R1U fungi Aspergillus n/a << 3.5475 3.5155 388.8811 100 0 100 100.000 6589 R1U viral Lymphocryptovirus n/a << 0.4965 0.4913 46.6036 24 16 8 13.974 1603 R2U bacteria Alcanivorax n/a 0.0711 0.0703 6.6700 2 0 2 2.000 105 R2U bacteria Eubacterium n/a 0.0355 0.0352 3.3350 1 0 1 1.000 60 R2U bacteria Rhizobacter n/a << 2.4515 2.4257 230.1166 69 0 69 69.000 4467 R2U viral Lymphocryptovirus n/a