# 2020/05/25 03:32:35 # HUMAN Target (Host) organism # DNA Sequencing source # ERR016209 Replication ID # SRP001294 Sample ID # ERR016209_1.filt Read1 # ERR016209_2.filt Read2 R1 31164325 (100.0000%) [A] Total raw reads R1 26283542 (84.3386%) [B] Total reads after QC of (A) R1 R1 26283542 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 46973666 (89.3595%) [B-3] Total mapped reads of (B-2)x2 R1 5593418 (10.6405%) [B-4] Total unmapped reads of (B-2)x2 R1 23222126 (88.3523%) [F] Total mapped reads of (B-2) R1 3061416 (11.6477%) [G] Total unmapped reads of (B-2) R1 348795 (1.3270%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 25934747 (98.6730%) [J] Total mapped reads to host genome ( (B-2)-H) R1 6036 (0.0230%) [I-1] Total mapped reads of (H) to exo. genomes R1 232.738 [I-3] RPMH of (I-1) R1 229.649 [I-4] PPM of (I-1) R1 17305.294 [I-5] RPMU of (I-1) R1 46 (0.0002%) [I-6] Total chimeric reads of (I-1) R1 1.774 [I-7] RPMH of (I-6) R1 1.750 [I-8] PPM of (I-6) R1 131.883 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 31164325 (100.0000%) [A] Total raw reads R2 26283542 (84.3386%) [B] Total reads after QC of (A) R2 R2 26283542 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 46973666 (89.3595%) [B-3] Total mapped reads of (B-2)x2 R2 5593418 (10.6405%) [B-4] Total unmapped reads of (B-2)x2 R2 23751540 (90.3666%) [F] Total mapped reads of (B-2) R2 2532002 (9.6334%) [G] Total unmapped reads of (B-2) R2 246748 (0.9388%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 26036794 (99.0612%) [J] Total mapped reads to host genome ( (B-2)-H) R2 6189 (0.0235%) [I-1] Total mapped reads of (H) to exo. genomes R2 237.702 [I-3] RPMH of (I-1) R2 235.471 [I-4] PPM of (I-1) R2 25082.270 [I-5] RPMU of (I-1) R2 29 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 1.114 [I-7] RPMH of (I-6) R2 1.103 [I-8] PPM of (I-6) R2 117.529 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2.8594 2.8215 212.6149 138 102 36 74.159 11130 ALL_R1 bacteria Alcanivorax 4.15e-81 << 226.6072 223.6000 16849.4388 5877 0 5877 5877.000 475692 ALL_R1 viral Lymphocryptovirus 0.0 << 7.0061 6.9404 739.2846 347 267 80 182.417 21897 ALL_R2 bacteria Alcanivorax 0.0 4.4044 4.3630 464.7495 262 239 23 114.676 16686 ALL_R2 bacteria Tetrasphaera 9.52e-23 << 222.7233 220.6324 23501.7102 5799 0 5799 5799.000 345148 ALL_R2 viral Lymphocryptovirus 0.0 << 0.5775 0.5699 42.9421 30 24 6 14.978 2402 R1U bacteria Alcanivorax n/a 0.0386 0.0380 2.8670 1 0 1 1.000 81 R1U bacteria Arthrobacter n/a << 0.5398 0.5327 40.1382 14 0 14 14.000 1134 R1U viral Lymphocryptovirus n/a << 0.2568 0.2543 27.0924 11 7 4 6.685 729 R2U bacteria Alcanivorax n/a 0.1799 0.1782 18.9870 9 7 2 4.685 563 R2U bacteria Tetrasphaera n/a 0.0384 0.0380 4.0527 1 0 1 1.000 37 R2U bacteria Actinocatenispora n/a 0.0384 0.0380 4.0527 1 0 1 1.000 36 R2U bacteria Polymorphum n/a << 0.5377 0.5327 56.7380 14 0 14 14.000 839 R2U viral Lymphocryptovirus n/a