# 2020/05/18 11:18:59 # HUMAN Target (Host) organism # DNA Sequencing source # SRR038563 Replication ID # SRP001294 Sample ID # SRR038563_1.filt Read1 # SRR038563_2.filt Read2 R1 18062762 (100.0000%) [A] Total raw reads R1 16314215 (90.3196%) [B] Total reads after QC of (A) R1 R1 16314215 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 28496349 (87.3359%) [B-3] Total mapped reads of (B-2)x2 R1 4132081 (12.6641%) [B-4] Total unmapped reads of (B-2)x2 R1 14262063 (87.4211%) [F] Total mapped reads of (B-2) R1 2052152 (12.5789%) [G] Total unmapped reads of (B-2) R1 1112124 (6.8169%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 15202091 (93.1831%) [J] Total mapped reads to host genome ( (B-2)-H) R1 17938 (0.1100%) [I-1] Total mapped reads of (H) to exo. genomes R1 1179.969 [I-3] RPMH of (I-1) R1 1099.532 [I-4] PPM of (I-1) R1 16129.496 [I-5] RPMU of (I-1) R1 203 (0.0012%) [I-6] Total chimeric reads of (I-1) R1 13.353 [I-7] RPMH of (I-6) R1 12.443 [I-8] PPM of (I-6) R1 182.534 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 18062762 (100.0000%) [A] Total raw reads R2 16314215 (90.3196%) [B] Total reads after QC of (A) R2 R2 16314215 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 28496349 (87.3359%) [B-3] Total mapped reads of (B-2)x2 R2 4132081 (12.6641%) [B-4] Total unmapped reads of (B-2)x2 R2 14234286 (87.2508%) [F] Total mapped reads of (B-2) R2 2079929 (12.7492%) [G] Total unmapped reads of (B-2) R2 1110792 (6.8087%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 15203423 (93.1913%) [J] Total mapped reads to host genome ( (B-2)-H) R2 17901 (0.1097%) [I-1] Total mapped reads of (H) to exo. genomes R2 1177.432 [I-3] RPMH of (I-1) R2 1097.264 [I-4] PPM of (I-1) R2 16115.528 [I-5] RPMU of (I-1) R2 126 (0.0008%) [I-6] Total chimeric reads of (I-1) R2 8.288 [I-7] RPMH of (I-6) R2 7.723 [I-8] PPM of (I-6) R2 113.433 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1.9734 1.8389 26.9754 30 0 30 30.000 2279 ALL_R1 bacteria Eubacterium 4.84e-05 1.7346 1.6163 23.7105 27 1 26 26.369 1946 ALL_R1 bacteria Bradyrhizobium 3.79e-05 << 1171.1547 1091.3182 16009.0062 17804 0 17804 17804.000 1295154 ALL_R1 viral Lymphocryptovirus 0.0 << 1.6272 1.5164 22.2715 26 2 24 24.739 1759 ALL_R2 bacteria Bradyrhizobium 2.25e-04 << 1169.4735 1089.8471 16006.5971 17780 0 17780 17780.000 1252902 ALL_R2 viral Lymphocryptovirus 0.0 << 13.0245 12.1367 178.0377 198 0 198 198.000 14502 R1U viral Lymphocryptovirus n/a << 8.2876 7.7233 113.4326 126 0 126 126.000 8959 R2U viral Lymphocryptovirus n/a