# 2020/05/18 03:40:46 # HUMAN Target (Host) organism # DNA Sequencing source # SRR035485 Replication ID # SRP001294 Sample ID # SRR035485_1.filt Read1 # SRR035485_2.filt Read2 R1 16563070 (100.0000%) [A] Total raw reads R1 84649 (0.5111%) [B] Total reads after QC of (A) R1 R1 84649 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 67018 (39.5858%) [B-3] Total mapped reads of (B-2)x2 R1 102280 (60.4142%) [B-4] Total unmapped reads of (B-2)x2 R1 48601 (57.4147%) [F] Total mapped reads of (B-2) R1 36048 (42.5853%) [G] Total unmapped reads of (B-2) R1 6202 (7.3267%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 78447 (92.6733%) [J] Total mapped reads to host genome ( (B-2)-H) R1 42 (0.0496%) [I-1] Total mapped reads of (H) to exo. genomes R1 535.393 [I-3] RPMH of (I-1) R1 496.167 [I-4] PPM of (I-1) R1 6772.009 [I-5] RPMU of (I-1) R1 8 (0.0095%) [I-6] Total chimeric reads of (I-1) R1 101.980 [I-7] RPMH of (I-6) R1 94.508 [I-8] PPM of (I-6) R1 1289.906 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 16563070 (100.0000%) [A] Total raw reads R2 84649 (0.5111%) [B] Total reads after QC of (A) R2 R2 84649 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 67018 (39.5858%) [B-3] Total mapped reads of (B-2)x2 R2 102280 (60.4142%) [B-4] Total unmapped reads of (B-2)x2 R2 18417 (21.7569%) [F] Total mapped reads of (B-2) R2 66232 (78.2431%) [G] Total unmapped reads of (B-2) R2 40630 (47.9982%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 44019 (52.0018%) [J] Total mapped reads to host genome ( (B-2)-H) R2 32 (0.0378%) [I-1] Total mapped reads of (H) to exo. genomes R2 726.959 [I-3] RPMH of (I-1) R2 378.032 [I-4] PPM of (I-1) R2 787.595 [I-5] RPMU of (I-1) R2 20 (0.0236%) [I-6] Total chimeric reads of (I-1) R2 454.349 [I-7] RPMH of (I-6) R2 236.270 [I-8] PPM of (I-6) R2 492.247 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 12.7475 11.8135 161.2383 1 0 1 1.000 40 ALL_R1 fungi Malassezia 6.55e-242 << 484.4035 448.9126 6127.0558 38 0 38 38.000 1471 ALL_R1 viral Lymphocryptovirus 0.0 12.7475 11.8135 161.2383 1 0 1 1.000 37 ALL_R1 viral Phix174microvirus 2.34e-187 << 340.7619 177.2023 369.1853 15 0 15 15.000 576 ALL_R2 bacteria Alcanivorax 0.0 22.7175 11.8135 24.6124 1 0 1 1.000 39 ALL_R2 bacteria Sanguibacteroides 0.0 22.7175 11.8135 24.6124 1 0 1 1.000 39 ALL_R2 bacteria Ideonella n/a 22.7175 11.8135 24.6124 1 0 1 1.000 36 ALL_R2 bacteria Turicibacter 0.0 22.7175 11.8135 24.6124 1 0 1 1.000 36 ALL_R2 bacteria Gottschalkia 0.0 << 90.8699 47.2540 98.4494 4 0 4 4.000 152 ALL_R2 fungi Malassezia 0.0 45.4349 23.6270 49.2247 2 0 2 2.000 75 ALL_R2 fungi Pneumocystis 0.0 22.7175 11.8135 24.6124 1 0 1 1.000 39 ALL_R2 fungi Tuber 0.0 22.7175 11.8135 24.6124 1 0 1 1.000 39 ALL_R2 fungi Scedosporium 0.0 22.7175 11.8135 24.6124 1 0 1 1.000 39 ALL_R2 fungi Eutypa 0.0 22.7175 11.8135 24.6124 1 0 1 1.000 38 ALL_R2 fungi Mitosporidium 0.0 << 68.1524 35.4405 73.8371 3 0 3 3.000 117 ALL_R2 viral Lymphocryptovirus 0.0 << 101.9797 94.5079 1289.9065 8 0 8 8.000 307 R1U viral Lymphocryptovirus n/a << 136.3048 70.8809 147.6741 6 0 6 6.000 229 R2U bacteria Alcanivorax n/a 22.7175 11.8135 24.6124 1 0 1 1.000 39 R2U bacteria Ideonella n/a 22.7175 11.8135 24.6124 1 0 1 1.000 36 R2U bacteria Turicibacter n/a 22.7175 11.8135 24.6124 1 0 1 1.000 36 R2U bacteria Gottschalkia n/a << 90.8699 47.2540 98.4494 4 0 4 4.000 152 R2U fungi Malassezia n/a 45.4349 23.6270 49.2247 2 0 2 2.000 75 R2U fungi Pneumocystis n/a 22.7175 11.8135 24.6124 1 0 1 1.000 39 R2U fungi Eutypa n/a 22.7175 11.8135 24.6124 1 0 1 1.000 38 R2U fungi Mitosporidium n/a << 68.1524 35.4405 73.8371 3 0 3 3.000 117 R2U viral Lymphocryptovirus n/a