# 2018/03/04 23:51:59 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR429EWK_1 Replication ID # ENCSR429EWK Sample ID # ENCFF150OQH Read1 # ENCFF460GHU Read2 R1 41203759 (100.0000%) [A] Total raw reads R1 39740063 (96.4477%) [B] Total reads after QC of (A) R1 R1 39740063 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 75886072 (95.4780%) [B-3] Total mapped reads of (B-2)x2 R1 3594054 (4.5220%) [B-4] Total unmapped reads of (B-2)x2 R1 37989769 (95.5956%) [F] Total mapped reads of (B-2) R1 1750294 (4.4044%) [G] Total unmapped reads of (B-2) R1 419338 (1.0552%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 39320725 (98.9448%) [J] Total mapped reads to host genome ( (B-2)-H) R1 221055 (0.5563%) [I-1] Total mapped reads of (H) to exo. genomes R1 5621.845 [I-3] RPMH of (I-1) R1 5562.523 [I-4] PPM of (I-1) R1 79 (0.0002%) [I-5] Total chimeric reads of (I-1) R1 2.009 [I-7] RPMH of (I-5) R1 1.988 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 41203759 (100.0000%) [A] Total raw reads R2 39740063 (96.4477%) [B] Total reads after QC of (A) R2 R2 39740063 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 75886072 (95.4780%) [B-3] Total mapped reads of (B-2)x2 R2 3594054 (4.5220%) [B-4] Total unmapped reads of (B-2)x2 R2 37896303 (95.3605%) [F] Total mapped reads of (B-2) R2 1843760 (4.6395%) [G] Total unmapped reads of (B-2) R2 428489 (1.0782%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 39311574 (98.9218%) [J] Total mapped reads to host genome ( (B-2)-H) R2 220219 (0.5541%) [I-1] Total mapped reads of (H) to exo. genomes R2 5601.887 [I-3] RPMH of (I-1) R2 5541.486 [I-4] PPM of (I-1) R2 74 (0.0002%) [I-5] Total chimeric reads of (I-1) R2 1.882 [I-7] RPMH of (I-5) R2 1.862 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2612.5322 2584.9647 105370 3073 102297 102726.660 10533012 ALL_R1 bacteria Bacillus 0.0 881.4469 872.1459 114073 105146 8927 34659.132 11360359 ALL_R1 bacteria Pseudomonas 0.0 12.0031 11.8764 1304 1029 275 471.970 129999 ALL_R1 bacteria Cutibacterium 9.55e-40 << 2611.8136 2583.6523 104842 2438 102404 102674.505 10414840 ALL_R2 bacteria Bacillus 0.0 914.5052 904.6447 114146 104833 9313 35950.637 11080929 ALL_R2 bacteria Pseudomonas 0.0 16.5333 16.3551 5203 5120 83 649.952 511939 ALL_R2 bacteria Escherichia 4.78e-04 11.6030 11.4779 1330 1065 265 456.133 130291 ALL_R2 bacteria Cutibacterium 4.02e-36 4.0862 4.0421 1633 1510 123 160.633 159536 ALL_R2 bacteria Acidovorax 2.56e-04 << 1.0681 1.0569 45 3 42 42.000 4315 R1U bacteria Bacillus n/a FIP1L1(5);RN7SL2(5);RHOB(4);KMT2C(3);RN7SL1(3) << 1.2041 1.1911 49 2 47 47.336 4660 R2U bacteria Bacillus n/a RN7SL2(5);ST3GAL3(5);POU2F1(4);SLC7A5(3);DIP2B(3)