# 2020/05/02 01:08:04 # HUMAN Target (Host) organism # DNA Sequencing source # ERR006316 Replication ID # SRP000540 Sample ID # ERR006316_1.filt Read1 # ERR006316_2.filt Read2 R1 6799306 (100.0000%) [A] Total raw reads R1 229649 (3.3775%) [B] Total reads after QC of (A) R1 R1 229649 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 287274 (62.5463%) [B-3] Total mapped reads of (B-2)x2 R1 172024 (37.4537%) [B-4] Total unmapped reads of (B-2)x2 R1 145120 (63.1921%) [F] Total mapped reads of (B-2) R1 84529 (36.8079%) [G] Total unmapped reads of (B-2) R1 41084 (17.8899%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 188565 (82.1101%) [J] Total mapped reads to host genome ( (B-2)-H) R1 284 (0.1237%) [I-1] Total mapped reads of (H) to exo. genomes R1 1506.112 [I-3] RPMH of (I-1) R1 1236.670 [I-4] PPM of (I-1) R1 6912.667 [I-5] RPMU of (I-1) R1 14 (0.0061%) [I-6] Total chimeric reads of (I-1) R1 74.245 [I-7] RPMH of (I-6) R1 60.963 [I-8] PPM of (I-6) R1 340.765 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 6799306 (100.0000%) [A] Total raw reads R2 229649 (3.3775%) [B] Total reads after QC of (A) R2 R2 229649 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 287274 (62.5463%) [B-3] Total mapped reads of (B-2)x2 R2 172024 (37.4537%) [B-4] Total unmapped reads of (B-2)x2 R2 142154 (61.9006%) [F] Total mapped reads of (B-2) R2 87495 (38.0994%) [G] Total unmapped reads of (B-2) R2 46675 (20.3245%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 182974 (79.6755%) [J] Total mapped reads to host genome ( (B-2)-H) R2 226 (0.0984%) [I-1] Total mapped reads of (H) to exo. genomes R2 1235.148 [I-3] RPMH of (I-1) R2 984.110 [I-4] PPM of (I-1) R2 4841.993 [I-5] RPMU of (I-1) R2 12 (0.0052%) [I-6] Total chimeric reads of (I-1) R2 65.583 [I-7] RPMH of (I-6) R2 52.254 [I-8] PPM of (I-6) R2 257.097 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 102.0709 83.8105 468.4792 37 30 7 19.247 1332 ALL_R1 bacteria Alcanivorax 0.0 70.2516 57.6837 322.4370 31 30 1 13.247 1116 ALL_R1 bacteria Eubacterium 1.29e-11 15.9096 13.0634 73.0211 3 0 3 3.000 108 ALL_R1 bacteria Serratia 2.91e-46 15.9096 13.0634 73.0211 3 0 3 3.000 108 ALL_R1 bacteria Clostridium 9.91e-16 10.6064 8.7089 48.6808 2 0 2 2.000 72 ALL_R1 bacteria Corynebacterium 1.41e-44 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Viridibacillus 1.68e-15 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Thermithiobacillus 1.68e-15 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Thauera 1.41e-27 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Succinivibrio 7.52e-10 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Staphylococcus 1.68e-15 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Spiroplasma 7.52e-10 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Ruminiclostridium 1.68e-15 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Pseudoalteromonas 1.68e-15 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Prevotella 7.52e-10 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Polaribacter 1.68e-15 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Oceanicola 7.52e-10 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Lysinibacillus 9.66e-06 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Lactobacillus 1.68e-15 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Halanaerobium 7.52e-10 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Desulfotomaculum 4.03e-06 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Capnocytophaga 1.41e-27 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Arcobacter 2.81e-04 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 bacteria Alteromonas 4.03e-06 << 15.9096 13.0634 73.0211 3 0 3 3.000 108 ALL_R1 fungi Malassezia 2.26e-46 10.6064 8.7089 48.6808 2 0 2 2.000 72 ALL_R1 fungi Aspergillus 1.11e-39 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 fungi Tetrapisispora 1.47e-05 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 fungi Pneumocystis 9.10e-07 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 fungi Nosema 4.96e-09 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 fungi Kluyveromyces 9.66e-06 5.3032 4.3545 24.3404 1 0 1 1.000 36 ALL_R1 fungi Eutypa 1.47e-05 << 1076.5519 883.9577 4941.0963 203 0 203 203.000 7308 ALL_R1 viral Lymphocryptovirus 0.0 << 56.4069 44.9425 221.1248 21 18 3 10.321 756 ALL_R2 bacteria Alcanivorax 3.27e-159 50.9417 40.5880 199.7001 20 18 2 9.321 720 ALL_R2 bacteria Eubacterium 2.12e-70 16.4559 13.1113 64.5099 4 1 3 3.011 144 ALL_R2 bacteria Serratia 2.97e-73 15.4394 12.3014 60.5249 5 3 2 2.825 180 ALL_R2 bacteria Streptomyces 1.77e-60 5.5254 4.4024 21.6604 2 1 1 1.011 72 ALL_R2 bacteria Nocardia 3.25e-15 5.5254 4.4024 21.6604 2 1 1 1.011 72 ALL_R2 bacteria Mycoplasma 4.00e-05 5.5254 4.4024 21.6604 2 1 1 1.011 72 ALL_R2 bacteria Bacillus 4.78e-14 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 bacteria Ureaplasma 7.05e-43 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 bacteria Pseudomonas 6.76e-18 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 bacteria Pedosphaera 1.42e-08 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 bacteria Mucispirillum 4.30e-09 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 bacteria Fischerella 6.76e-18 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 bacteria Caulobacter 6.76e-18 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 bacteria Arsenophonus 4.30e-09 << 16.3958 13.0634 64.2742 3 0 3 3.000 108 ALL_R2 fungi Malassezia 9.80e-74 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 fungi Serpula 6.76e-18 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 fungi Laccaria 7.05e-43 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 fungi Fonsecaea 4.78e-14 5.4653 4.3545 21.4247 1 0 1 1.000 36 ALL_R2 fungi Aspergillus 6.24e-15 << 961.8853 766.3870 3770.7552 176 0 176 176.000 6336 ALL_R2 viral Lymphocryptovirus 0.0 << 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Thermithiobacillus n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Staphylococcus n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Spiroplasma n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Sodalis n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Ruminiclostridium n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Prevotella n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Lysinibacillus n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U bacteria Capnocytophaga n/a << 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U fungi Pneumocystis n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U fungi Nosema n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U fungi Kluyveromyces n/a 5.3032 4.3545 24.3404 1 0 1 1.000 36 R1U fungi Eutypa n/a << 7.6896 6.1267 30.1446 2 1 1 1.407 72 R2U bacteria Eubacterium n/a 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U bacteria Ureaplasma n/a 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U bacteria Pedosphaera n/a 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U bacteria Mucispirillum n/a 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U bacteria Fischerella n/a 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U bacteria Caulobacter n/a 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U bacteria Bacillus n/a << 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U fungi Malassezia n/a 5.4653 4.3545 21.4247 1 0 1 1.000 36 R2U fungi Fonsecaea n/a