# 2018/03/21 04:45:29 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR373BDG_1 Replication ID # ENCSR373BDG Sample ID # ENCFF109IUU Read1 # ENCFF322VHJ Read2 R1 106249338 (100.0000%) [A] Total raw reads R1 101950156 (95.9537%) [B] Total reads after QC of (A) R1 R1 101950156 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 192347002 (94.3338%) [B-3] Total mapped reads of (B-2)x2 R1 11553310 (5.6662%) [B-4] Total unmapped reads of (B-2)x2 R1 96217870 (94.3774%) [F] Total mapped reads of (B-2) R1 5732286 (5.6226%) [G] Total unmapped reads of (B-2) R1 2775845 (2.7227%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 99174311 (97.2773%) [J] Total mapped reads to host genome ( (B-2)-H) R1 1432825 (1.4054%) [I-1] Total mapped reads of (H) to exo. genomes R1 14447.542 [I-3] RPMH of (I-1) R1 14054.172 [I-4] PPM of (I-1) R1 552 (0.0005%) [I-5] Total chimeric reads of (I-1) R1 5.566 [I-7] RPMH of (I-5) R1 5.414 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 106249338 (100.0000%) [A] Total raw reads R2 101950156 (95.9537%) [B] Total reads after QC of (A) R2 R2 101950156 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 192347002 (94.3338%) [B-3] Total mapped reads of (B-2)x2 R2 11553310 (5.6662%) [B-4] Total unmapped reads of (B-2)x2 R2 96129132 (94.2903%) [F] Total mapped reads of (B-2) R2 5821024 (5.7097%) [G] Total unmapped reads of (B-2) R2 2830062 (2.7759%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 99120094 (97.2241%) [J] Total mapped reads to host genome ( (B-2)-H) R2 1435425 (1.4080%) [I-1] Total mapped reads of (H) to exo. genomes R2 14481.675 [I-3] RPMH of (I-1) R2 14079.674 [I-4] PPM of (I-1) R2 585 (0.0006%) [I-5] Total chimeric reads of (I-1) R2 5.902 [I-7] RPMH of (I-5) R2 5.738 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 7451.5073 7248.6216 750184 12249 737935 738998.106 74634131 ALL_R1 bacteria Bacillus 0.0 1284.1684 1249.2037 608955 596076 12879 127356.515 60519819 ALL_R1 bacteria Pseudomonas 0.0 580.5439 564.7371 67807 15169 52638 57575.037 6661228 ALL_R1 bacteria Sphingomonas 0.0 28.8129 28.0284 7629 7091 538 2857.498 752947 ALL_R1 bacteria Sphingobium 1.57e-04 18.1063 17.6133 6060 4453 1607 1795.681 601599 ALL_R1 bacteria Bradyrhizobium 0.0 << 7475.2052 7267.6989 750989 11143 739846 740943.040 74256316 ALL_R2 bacteria Bacillus 0.0 1210.7674 1177.1574 612373 597429 14944 120011.379 59209890 ALL_R2 bacteria Pseudomonas 0.0 596.2996 579.7468 66465 12793 53672 59105.277 6400199 ALL_R2 bacteria Sphingomonas 0.0 19.0705 18.5411 4898 3291 1607 1890.267 469948 ALL_R2 bacteria Bradyrhizobium 0.0 << 1007.0861 979.1301 573271 573270 1 99822.466 55407919 ALL_R2 viral Phix174microvirus n/a << 4.4989 4.3764 454 8 446 446.174 44533 R1U bacteria Bacillus n/a PCNX2(83);SRSF5(26);SFPQ(26);CYB5R4(25);PNRC2(15) << 3.9494 3.8397 416 27 389 391.463 39771 R2U bacteria Bacillus n/a TENM4(45);KDM3B(41);RNR2(40);LAMB1(33);GBA(27)