# 2018/03/13 20:24:11 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR878EUT_2 Replication ID # ENCSR878EUT Sample ID # ENCFF509JLH Read1 # ENCFF867UER Read2 R1 106140353 (100.0000%) [A] Total raw reads R1 102896272 (96.9436%) [B] Total reads after QC of (A) R1 R1 102896272 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 197395290 (95.9196%) [B-3] Total mapped reads of (B-2)x2 R1 8397254 (4.0804%) [B-4] Total unmapped reads of (B-2)x2 R1 98773592 (95.9934%) [F] Total mapped reads of (B-2) R1 4122680 (4.0066%) [G] Total unmapped reads of (B-2) R1 794748 (0.7724%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 102101524 (99.2276%) [J] Total mapped reads to host genome ( (B-2)-H) R1 429006 (0.4169%) [I-1] Total mapped reads of (H) to exo. genomes R1 4201.759 [I-3] RPMH of (I-1) R1 4169.306 [I-4] PPM of (I-1) R1 252 (0.0002%) [I-5] Total chimeric reads of (I-1) R1 2.468 [I-7] RPMH of (I-5) R1 2.449 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 106140353 (100.0000%) [A] Total raw reads R2 102896272 (96.9436%) [B] Total reads after QC of (A) R2 R2 102896272 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 197395290 (95.9196%) [B-3] Total mapped reads of (B-2)x2 R2 8397254 (4.0804%) [B-4] Total unmapped reads of (B-2)x2 R2 98621698 (95.8457%) [F] Total mapped reads of (B-2) R2 4274574 (4.1543%) [G] Total unmapped reads of (B-2) R2 854735 (0.8307%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 102041537 (99.1693%) [J] Total mapped reads to host genome ( (B-2)-H) R2 428505 (0.4164%) [I-1] Total mapped reads of (H) to exo. genomes R2 4199.319 [I-3] RPMH of (I-1) R2 4164.437 [I-4] PPM of (I-1) R2 223 (0.0002%) [I-5] Total chimeric reads of (I-1) R2 2.185 [I-7] RPMH of (I-5) R2 2.167 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1847.6384 1833.3677 193421 5397 188024 188646.698 19180456 ALL_R1 bacteria Bacillus 0.0 674.5251 669.3152 231748 221548 10200 68870.036 22644031 ALL_R1 bacteria Pseudomonas 0.0 14.6207 14.5077 5842 5605 237 1492.791 579254 ALL_R1 bacteria Sphingomonas 7.98e-15 9.0937 9.0235 2275 1703 572 928.484 224766 ALL_R1 bacteria Cutibacterium 7.23e-225 6.4396 6.3899 2571 2285 286 657.492 254203 ALL_R1 bacteria Corynebacterium 1.30e-18 << 1846.8166 1831.4755 193206 5097 188109 188452.003 19281384 ALL_R2 bacteria Bacillus 0.0 712.6555 706.7356 232205 220227 11978 72720.463 22373071 ALL_R2 bacteria Pseudomonas 0.0 6.4877 6.4338 2094 1656 438 662.013 206807 ALL_R2 bacteria Cutibacterium 2.13e-100 4.1979 4.1631 2149 1918 231 428.364 212655 ALL_R2 bacteria Corynebacterium 9.28e-06 2.7656 2.7426 2770 2573 197 282.204 273713 ALL_R2 bacteria Acidovorax 5.00e-04 << 1.1088 1.1003 122 9 113 113.213 11449 R1U bacteria Bacillus n/a FN1(21);RNR2(14);S100A16(11);RNR1(7);CCNG1(7) << 1.4406 1.4286 150 3 147 147.000 13417 R2U bacteria Bacillus n/a RNR2(29);LPIN2(20);RPPH1(8);TIE1(8);FN1(5)