# 2018/03/14 16:42:16 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR919MZM_1 Replication ID # ENCSR919MZM Sample ID # ENCFF126HPJ Read1 # ENCFF876WTO Read2 R1 88048572 (100.0000%) [A] Total raw reads R1 84383192 (95.8371%) [B] Total reads after QC of (A) R1 R1 84383192 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 157649189 (93.4127%) [B-3] Total mapped reads of (B-2)x2 R1 11117195 (6.5873%) [B-4] Total unmapped reads of (B-2)x2 R1 78818620 (93.4056%) [F] Total mapped reads of (B-2) R1 5564572 (6.5944%) [G] Total unmapped reads of (B-2) R1 3634620 (4.3073%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 80748572 (95.6927%) [J] Total mapped reads to host genome ( (B-2)-H) R1 1570281 (1.8609%) [I-1] Total mapped reads of (H) to exo. genomes R1 19446.548 [I-3] RPMH of (I-1) R1 18608.931 [I-4] PPM of (I-1) R1 779 (0.0009%) [I-5] Total chimeric reads of (I-1) R1 9.647 [I-7] RPMH of (I-5) R1 9.232 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 88048572 (100.0000%) [A] Total raw reads R2 84383192 (95.8371%) [B] Total reads after QC of (A) R2 R2 84383192 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 157649189 (93.4127%) [B-3] Total mapped reads of (B-2)x2 R2 11117195 (6.5873%) [B-4] Total unmapped reads of (B-2)x2 R2 78830569 (93.4198%) [F] Total mapped reads of (B-2) R2 5552623 (6.5802%) [G] Total unmapped reads of (B-2) R2 3640334 (4.3141%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 80742858 (95.6859%) [J] Total mapped reads to host genome ( (B-2)-H) R2 1573114 (1.8643%) [I-1] Total mapped reads of (H) to exo. genomes R2 19483.011 [I-3] RPMH of (I-1) R2 18642.504 [I-4] PPM of (I-1) R2 522 (0.0006%) [I-5] Total chimeric reads of (I-1) R2 6.465 [I-7] RPMH of (I-5) R2 6.186 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 14515.0852 13889.8799 1192162 20763 1171399 1172072.405 119698328 ALL_R1 bacteria Bacillus 0.0 729.7128 698.2820 328778 301240 27538 58923.268 32890662 ALL_R1 bacteria Pseudomonas 0.0 403.3855 386.0105 51011 23092 27919 32572.800 5084768 ALL_R1 bacteria Sphingomonas 0.0 78.6952 75.3055 15095 9030 6065 6354.521 1509145 ALL_R1 bacteria Bradyrhizobium 0.0 43.3498 41.4826 46503 45822 681 3500.431 4674983 ALL_R1 bacteria Escherichia 1.03e-45 35.5395 34.0087 14175 12542 1633 2869.765 1423987 ALL_R1 bacteria Cutibacterium 9.89e-203 << 14549.2047 13921.5446 1190365 15985 1174380 1174744.372 119450671 ALL_R2 bacteria Bacillus 0.0 720.9767 689.8734 330534 298576 31958 58213.720 32463611 ALL_R2 bacteria Pseudomonas 0.0 390.9111 374.0470 44552 17565 26987 31563.280 4380468 ALL_R2 bacteria Sphingomonas 0.0 74.0545 70.8597 12400 6651 5749 5979.368 1206964 ALL_R2 bacteria Bradyrhizobium 0.0 42.1686 40.3494 43039 42290 749 3404.815 4283977 ALL_R2 bacteria Escherichia 7.06e-69 37.8611 36.2277 13750 12119 1631 3057.013 1367296 ALL_R2 bacteria Cutibacterium 7.62e-201 28.5439 27.3125 13800 12692 1108 2304.715 1371805 ALL_R2 bacteria Corynebacterium 7.19e-07 << 5.8971 5.6431 565 89 476 476.184 55777 R1U bacteria Bacillus n/a RN7SL1(197);ZC3H7B(51);JAK1(47);GALNT1(33);SUZ12(15) << 5.6111 5.3691 461 8 453 453.058 43931 R2U bacteria Bacillus n/a RN7SL2(53);S100A16(44);RNR2(42);RNR1(29);AES(20)