# 2020/03/20 01:28:07 # HUMAN Target (Host) organism # RNA Sequencing source # ERR204985 Replication ID # ERR204985 Sample ID # ERR204985_1 Read1 # ERR204985_2 Read2 R1 24157268 (100.0000%) [A] Total raw reads R1 23592722 (97.6630%) [B] Total reads after QC of (A) R1 R1 23592722 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 43412486 (92.0040%) [B-3] Total mapped reads of (B-2)x2 R1 3772958 (7.9960%) [B-4] Total unmapped reads of (B-2)x2 R1 21739243 (92.1439%) [F] Total mapped reads of (B-2) R1 1853479 (7.8561%) [G] Total unmapped reads of (B-2) R1 156696 (0.6642%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 23436026 (99.3358%) [J] Total mapped reads to host genome ( (B-2)-H) R1 117955 (0.5000%) [I-1] Total mapped reads of (H) to exo. genomes R1 5033.063 [I-3] RPMH of (I-1) R1 4999.635 [I-4] PPM of (I-1) R1 752763.312 [I-5] RPMU of (I-1) R1 124 (0.0005%) [I-6] Total chimeric reads of (I-1) R1 5.291 [I-7] RPMH of (I-6) R1 5.256 [I-8] PPM of (I-6) R1 791.341 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 24157268 (100.0000%) [A] Total raw reads R2 23592722 (97.6630%) [B] Total reads after QC of (A) R2 R2 23592722 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 43412486 (92.0040%) [B-3] Total mapped reads of (B-2)x2 R2 3772958 (7.9960%) [B-4] Total unmapped reads of (B-2)x2 R2 21673243 (91.8641%) [F] Total mapped reads of (B-2) R2 1919479 (8.1359%) [G] Total unmapped reads of (B-2) R2 180106 (0.7634%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 23412616 (99.2366%) [J] Total mapped reads to host genome ( (B-2)-H) R2 117201 (0.4968%) [I-1] Total mapped reads of (H) to exo. genomes R2 5005.891 [I-3] RPMH of (I-1) R2 4967.676 [I-4] PPM of (I-1) R2 650733.457 [I-5] RPMU of (I-1) R2 155 (0.0007%) [I-6] Total chimeric reads of (I-1) R2 6.620 [I-7] RPMH of (I-6) R2 6.570 [I-8] PPM of (I-6) R2 860.604 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 640.2832 636.0306 95763.0954 21806 9941 11865 15005.694 1627539 ALL_R1 bacteria Nocardia 0.0 8.1282 8.0742 1215.6851 536 354 182 190.493 39838 ALL_R1 bacteria Cutibacterium 9.96e-77 5.4630 5.4268 817.0725 279 156 123 128.032 20474 ALL_R1 bacteria Methylobacterium 2.02e-43 3.4552 3.4323 516.7777 175 97 78 80.977 12867 ALL_R1 bacteria Cupriavidus 9.92e-06 << 13.4337 13.3445 2009.1962 339 31 308 314.833 24935 ALL_R1 fungi Saccharomyces 1.87e-252 << 3736.8110 3711.9922 558891.1012 87576 0 87576 87576.000 6476674 ALL_R1 viral Lymphocryptovirus 0.0 << 517.0173 513.0704 67208.9048 21700 15508 6192 12104.727 1609654 ALL_R2 bacteria Nocardia 0.0 8.1409 8.0788 1058.2657 534 351 183 190.600 39498 ALL_R2 bacteria Cutibacterium 3.45e-79 5.5401 5.4978 720.1759 281 157 124 129.708 20531 ALL_R2 bacteria Methylobacterium 3.65e-45 3.3073 3.2820 429.9246 325 256 69 77.432 24063 ALL_R2 bacteria Lactobacillus 3.53e-05 3.2652 3.2403 424.4612 157 84 73 76.448 11477 ALL_R2 bacteria Cupriavidus 8.89e-05 << 13.3907 13.2885 1740.7027 335 27 308 313.511 24703 ALL_R2 fungi Saccharomyces 2.81e-256 << 3716.6517 3688.2790 483140.7060 87017 1 87016 87016.540 6445814 ALL_R2 viral Lymphocryptovirus 0.0 << 0.5909 0.5870 88.3813 25 17 8 13.849 1792 R1U bacteria Nocardia n/a << 0.0427 0.0424 6.3818 1 0 1 1.000 39 R1U fungi Talaromyces n/a << 3.7122 3.6876 555.2152 87 0 87 87.000 6390 R1U viral Lymphocryptovirus n/a << 0.2582 0.2562 33.5580 15 13 2 6.044 1081 R2U bacteria Nocardia n/a 0.2578 0.2558 33.5136 14 13 1 6.036 563 R2U bacteria Novosphingobium n/a 0.0854 0.0848 11.1046 2 0 2 2.000 148 R2U bacteria Loktanella n/a 0.0658 0.0653 8.5505 2 1 1 1.540 87 R2U bacteria Nocardioides n/a 0.0466 0.0463 6.0631 3 2 1 1.092 183 R2U bacteria Pseudomonas n/a 0.0427 0.0424 5.5523 1 0 1 1.000 75 R2U bacteria Methylobacterium n/a 0.0427 0.0424 5.5523 1 0 1 1.000 74 R2U bacteria Lachnoclostridium n/a << 0.0854 0.0848 11.1046 3 1 2 2.000 189 R2U fungi Histoplasma n/a 0.0427 0.0424 5.5523 2 1 1 1.000 132 R2U fungi Pseudozyma n/a 0.0427 0.0424 5.5523 2 1 1 1.000 129 R2U fungi Clavispora n/a 0.0427 0.0424 5.5523 2 1 1 1.000 125 R2U fungi Colletotrichum n/a 0.0427 0.0424 5.5523 2 1 1 1.000 124 R2U fungi Aspergillus n/a 0.0427 0.0424 5.5523 2 1 1 1.000 118 R2U fungi Baudoinia n/a 0.0427 0.0424 5.5523 2 1 1 1.000 116 R2U fungi Zymoseptoria n/a 0.0427 0.0424 5.5523 2 1 1 1.000 113 R2U fungi Coprinopsis n/a 0.0427 0.0424 5.5523 2 1 1 1.000 113 R2U fungi Blastomyces n/a 0.0427 0.0424 5.5523 1 0 1 1.000 41 R2U fungi Aureobasidium n/a 0.0427 0.0424 5.5523 1 0 1 1.000 37 R2U fungi Verruconis n/a << 4.1004 4.0691 533.0194 96 0 96 96.000 6945 R2U viral Lymphocryptovirus n/a