# 2018/02/02 10:02:25 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR000AFE_1 Replication ID # ENCSR000AFE Sample ID # ENCFF001ROB Read1 # ENCFF001ROA Read2 R1 102763963 (100.0000%) [A] Total raw reads R1 100436437 (97.7351%) [B] Total reads after QC of (A) R1 R1 100436437 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 194006728 (96.5818%) [B-3] Total mapped reads of (B-2)x2 R1 6866146 (3.4182%) [B-4] Total unmapped reads of (B-2)x2 R1 97239515 (96.8170%) [F] Total mapped reads of (B-2) R1 3196922 (3.1830%) [G] Total unmapped reads of (B-2) R1 301822 (0.3005%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 100134615 (99.6995%) [J] Total mapped reads to host genome ( (B-2)-H) R1 166070 (0.1653%) [I-1] Total mapped reads of (H) to exo. genomes R1 1658.467 [I-3] RPMH of (I-1) R1 1653.484 [I-4] PPM of (I-1) R1 192 (0.0002%) [I-5] Total chimeric reads of (I-1) R1 1.917 [I-7] RPMH of (I-5) R1 1.912 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 102763963 (100.0000%) [A] Total raw reads R2 100436437 (97.7351%) [B] Total reads after QC of (A) R2 R2 100436437 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 194006728 (96.5818%) [B-3] Total mapped reads of (B-2)x2 R2 6866146 (3.4182%) [B-4] Total unmapped reads of (B-2)x2 R2 96767213 (96.3467%) [F] Total mapped reads of (B-2) R2 3669224 (3.6533%) [G] Total unmapped reads of (B-2) R2 508988 (0.5068%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 99927449 (99.4932%) [J] Total mapped reads to host genome ( (B-2)-H) R2 159911 (0.1592%) [I-1] Total mapped reads of (H) to exo. genomes R2 1600.271 [I-3] RPMH of (I-1) R2 1592.161 [I-4] PPM of (I-1) R2 43 (0.0000%) [I-5] Total chimeric reads of (I-1) R2 0.430 [I-7] RPMH of (I-5) R2 0.428 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 388.9911 387.8222 39651 816 38835 38951.477 3925971 ALL_R1 bacteria Bacillus 0.0 12.5623 12.5246 2202 1885 317 1257.922 193813 ALL_R1 bacteria Escherichia 0.0 << 391.7552 389.7699 39624 607 39017 39147.096 3919911 ALL_R2 bacteria Bacillus 0.0 2.0466 2.0362 613 510 103 204.507 59472 ALL_R2 bacteria Cutibacterium 9.97e-05