# 2020/03/25 08:31:36 # HUMAN Target (Host) organism # RNA Sequencing source # ERR204917 Replication ID # ERR204917 Sample ID # ERR204917_1 Read1 # ERR204917_2 Read2 R1 19524803 (100.0000%) [A] Total raw reads R1 18962232 (97.1187%) [B] Total reads after QC of (A) R1 R1 18962232 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 34528048 (91.0443%) [B-3] Total mapped reads of (B-2)x2 R1 3396416 (8.9557%) [B-4] Total unmapped reads of (B-2)x2 R1 17321003 (91.3447%) [F] Total mapped reads of (B-2) R1 1641229 (8.6553%) [G] Total unmapped reads of (B-2) R1 71008 (0.3745%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 18891224 (99.6255%) [J] Total mapped reads to host genome ( (B-2)-H) R1 43629 (0.2301%) [I-1] Total mapped reads of (H) to exo. genomes R1 2309.485 [I-3] RPMH of (I-1) R1 2300.837 [I-4] PPM of (I-1) R1 614423.727 [I-5] RPMU of (I-1) R1 139 (0.0007%) [I-6] Total chimeric reads of (I-1) R1 7.358 [I-7] RPMH of (I-6) R1 7.330 [I-8] PPM of (I-6) R1 1957.526 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 19524803 (100.0000%) [A] Total raw reads R2 18962232 (97.1187%) [B] Total reads after QC of (A) R2 R2 18962232 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 34528048 (91.0443%) [B-3] Total mapped reads of (B-2)x2 R2 3396416 (8.9557%) [B-4] Total unmapped reads of (B-2)x2 R2 17207045 (90.7438%) [F] Total mapped reads of (B-2) R2 1755187 (9.2562%) [G] Total unmapped reads of (B-2) R2 113027 (0.5961%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 18849205 (99.4039%) [J] Total mapped reads to host genome ( (B-2)-H) R2 43371 (0.2287%) [I-1] Total mapped reads of (H) to exo. genomes R2 2300.946 [I-3] RPMH of (I-1) R2 2287.231 [I-4] PPM of (I-1) R2 383722.473 [I-5] RPMU of (I-1) R2 116 (0.0006%) [I-6] Total chimeric reads of (I-1) R2 6.154 [I-7] RPMH of (I-6) R2 6.117 [I-8] PPM of (I-6) R2 1026.303 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 360.7053 359.3546 95963.3422 8037 2183 5854 6814.165 594772 ALL_R1 bacteria Nocardia 0.0 3.3675 3.3549 895.9131 87 28 59 63.617 6406 ALL_R1 bacteria Escherichia 1.51e-19 << 1785.4322 1778.7463 475002.8166 33729 0 33729 33729.000 2481930 ALL_R1 viral Lymphocryptovirus 0.0 << 357.9214 355.7880 59689.5786 7978 2158 5820 6746.534 581613 ALL_R2 bacteria Nocardia 0.0 3.5584 3.5372 593.4246 77 13 64 67.073 5540 ALL_R2 bacteria Escherichia 1.95e-24 << 1780.3403 1769.7284 296902.5100 33558 0 33558 33558.000 2441877 ALL_R2 viral Lymphocryptovirus 0.0 << 0.4125 0.4110 109.7482 10 4 6 7.793 678 R1U bacteria Nocardia n/a << 3.7584 3.7443 999.8873 71 0 71 71.000 5156 R1U viral Lymphocryptovirus n/a << 0.3714 0.3692 61.9321 7 0 7 7.000 524 R2U bacteria Nocardia n/a 0.0581 0.0578 9.6968 3 2 1 1.096 178 R2U bacteria Nocardioides n/a 0.0531 0.0527 8.8474 1 0 1 1.000 36 R2U bacteria Methylobacterium n/a 0.0531 0.0527 8.8474 1 0 1 1.000 36 R2U bacteria Amycolatopsis n/a << 0.1592 0.1582 26.5423 4 1 3 3.000 212 R2U fungi Lodderomyces n/a 0.1117 0.1110 18.6239 5 3 2 2.105 266 R2U fungi Aspergillus n/a 0.0824 0.0819 13.7401 4 3 1 1.553 219 R2U fungi Leptosphaeria n/a 0.0586 0.0583 9.7764 4 3 1 1.105 231 R2U fungi Parastagonospora n/a 0.0535 0.0532 8.9271 3 2 1 1.009 189 R2U fungi Moesziomyces n/a 0.0535 0.0532 8.9271 3 2 1 1.009 189 R2U fungi Laccaria n/a 0.0535 0.0532 8.9271 2 1 1 1.009 96 R2U fungi Cutaneotrichosporon n/a 0.0531 0.0527 8.8474 2 1 1 1.000 111 R2U fungi Neofusicoccum n/a 0.0531 0.0527 8.8474 2 1 1 1.000 106 R2U fungi Pseudocercospora n/a 0.0531 0.0527 8.8474 2 1 1 1.000 106 R2U fungi Naumovozyma n/a 0.0531 0.0527 8.8474 1 0 1 1.000 75 R2U fungi Gaeumannomyces n/a 0.0531 0.0527 8.8474 1 0 1 1.000 43 R2U fungi Scedosporium n/a << 4.0851 4.0607 681.2532 77 0 77 77.000 5620 R2U viral Lymphocryptovirus n/a