# 2018/01/31 17:06:16 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR000AFC_1 Replication ID # ENCSR000AFC Sample ID # ENCFF002BUL Read1 # ENCFF002BUN Read2 R1 115731418 (100.0000%) [A] Total raw reads R1 107006723 (92.4613%) [B] Total reads after QC of (A) R1 R1 107006723 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 187273590 (87.5055%) [B-3] Total mapped reads of (B-2)x2 R1 26739856 (12.4945%) [B-4] Total unmapped reads of (B-2)x2 R1 97826488 (91.4209%) [F] Total mapped reads of (B-2) R1 9180235 (8.5791%) [G] Total unmapped reads of (B-2) R1 4453965 (4.1623%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 102552758 (95.8377%) [J] Total mapped reads to host genome ( (B-2)-H) R1 785979 (0.7345%) [I-1] Total mapped reads of (H) to exo. genomes R1 7664.143 [I-3] RPMH of (I-1) R1 7345.137 [I-4] PPM of (I-1) R1 1935 (0.0018%) [I-5] Total chimeric reads of (I-1) R1 18.868 [I-7] RPMH of (I-5) R1 18.083 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 115731418 (100.0000%) [A] Total raw reads R2 107006723 (92.4613%) [B] Total reads after QC of (A) R2 R2 107006723 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 187273590 (87.5055%) [B-3] Total mapped reads of (B-2)x2 R2 26739856 (12.4945%) [B-4] Total unmapped reads of (B-2)x2 R2 89447102 (83.5902%) [F] Total mapped reads of (B-2) R2 17559621 (16.4098%) [G] Total unmapped reads of (B-2) R2 8232594 (7.6935%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 98774129 (92.3065%) [J] Total mapped reads to host genome ( (B-2)-H) R2 750367 (0.7012%) [I-1] Total mapped reads of (H) to exo. genomes R2 7596.797 [I-3] RPMH of (I-1) R2 7012.335 [I-4] PPM of (I-1) R2 1590 (0.0015%) [I-5] Total chimeric reads of (I-1) R2 16.097 [I-7] RPMH of (I-5) R2 14.859 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2913.0811 2791.8293 315502 17720 297782 298744.500 31571928 ALL_R1 bacteria Bacillus 0.0 1572.9343 1507.4637 314425 302122 12303 161308.753 31363761 ALL_R1 bacteria Burkholderia 0.0 1149.1804 1101.3478 261745 260745 1000 117851.621 26098687 ALL_R1 bacteria Nocardioides 0.0 871.1793 834.9180 172764 170552 2212 89341.837 17229276 ALL_R1 bacteria Exiguobacterium 0.0 827.1667 792.7374 152886 151636 1250 84828.228 15288025 ALL_R1 bacteria Tenacibaculum 0.0 585.3142 560.9515 148588 147969 619 60025.583 14833722 ALL_R1 bacteria Erythrobacter 3.47e-171 15.2768 14.6409 6417 5958 459 1566.674 610568 ALL_R1 bacteria Corynebacterium 5.66e-04 13.1080 12.5624 6256 5751 505 1344.257 620771 ALL_R1 bacteria Lactobacillus 2.75e-42 10.8375 10.3864 4762 4173 589 1111.412 471530 ALL_R1 bacteria Cutibacterium 8.21e-67 9.5234 9.1270 4463 3919 544 976.655 442808 ALL_R1 bacteria Streptococcus 3.21e-41 << 86.5641 82.9610 32171 30593 1578 8877.389 3158630 ALL_R1 fungi Wallemia 0.0 35.7316 34.2443 34814 32067 2747 3664.370 3444188 ALL_R1 fungi Malassezia 0.0 << 2911.8005 2687.7803 294676 7713 286963 287610.563 28659824 ALL_R2 bacteria Bacillus 0.0 1591.2369 1468.8146 287645 281601 6044 157173.039 26641400 ALL_R2 bacteria Burkholderia 0.0 1359.5261 1254.9305 257315 255574 1741 134286.004 24272918 ALL_R2 bacteria Nocardioides 0.0 853.2512 787.6060 149708 148027 1681 84279.140 13918932 ALL_R2 bacteria Exiguobacterium 0.0 779.6599 719.6766 170183 169155 1028 77010.230 16171384 ALL_R2 bacteria Cellulomonas 0.0 683.9607 631.3400 112069 109060 3009 67557.627 10013861 ALL_R2 bacteria Tenacibaculum 0.0 527.7526 487.1498 123032 122607 425 52128.301 11739784 ALL_R2 bacteria Erythrobacter 1.60e-41 12.3746 11.4225 5040 4516 524 1222.286 455266 ALL_R2 bacteria Lactobacillus 6.73e-61 9.1113 8.4103 3675 3104 571 899.959 335335 ALL_R2 bacteria Streptococcus 5.55e-64 8.5846 7.9242 4036 3495 541 847.941 363771 ALL_R2 bacteria Cutibacterium 1.84e-57 3.7716 3.4814 1574 1213 361 372.534 143997 ALL_R2 bacteria Vogesella 6.73e-05 << 176.3933 162.8224 41627 33751 7876 17423.092 3763775 ALL_R2 fungi Wallemia 0.0 93.5171 86.3223 40787 37347 3440 9237.066 3416679 ALL_R2 fungi Malassezia 0.0 << 212.9673 196.5826 96556 96555 1 21035.662 7781651 ALL_R2 viral Phix174microvirus n/a 51.6427 47.6696 6665 6472 193 5100.966 658479 ALL_R2 viral Lambdavirus 7.30e-21 << 11.2657 10.7967 1747 1566 181 1155.324 172681 R1U bacteria Nocardioides n/a SLCO5A1(1423);HFM1(247);ANKRD30BL(9);ANKRD11(6);MFSD14A(4) 7.9040 7.5750 1338 1313 25 810.580 131384 R1U bacteria Exiguobacterium n/a SLCO5A1(1011);HFM1(266);ANKRD11(6);RN7SL1(4);ANKRD30BL(4) 7.5770 7.2616 1287 1238 49 777.044 126596 R1U bacteria Burkholderia n/a SLCO5A1(901);HFM1(243);ANKRD30BL(46);ANKRD11(6);RN7SL1(4) << 5.5391 5.1130 1231 1222 9 547.124 105081 R2U bacteria Burkholderia n/a HFM1(501);SLCO5A1(451);ANKRD30BL(11);RN7SL2(10);RN7SK(6) 5.5283 5.1030 1191 1169 22 546.054 101450 R2U bacteria Exiguobacterium n/a HFM1(549);SLCO5A1(452);RN7SK(5);ATRX(5);RMRP(4)