# 2020/03/23 04:27:04 # HUMAN Target (Host) organism # DNA Sequencing source # SRR034928 Replication ID # SRP000547 Sample ID # SRR034928_1.filt Read1 # SRR034928_2.filt Read2 R1 30007749 (100.0000%) [A] Total raw reads R1 8220533 (27.3947%) [B] Total reads after QC of (A) R1 R1 8220533 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 15389388 (93.6033%) [B-3] Total mapped reads of (B-2)x2 R1 1051678 (6.3967%) [B-4] Total unmapped reads of (B-2)x2 R1 7701260 (93.6832%) [F] Total mapped reads of (B-2) R1 519273 (6.3168%) [G] Total unmapped reads of (B-2) R1 38198 (0.4647%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 8182335 (99.5353%) [J] Total mapped reads to host genome ( (B-2)-H) R1 12096 (0.1471%) [I-1] Total mapped reads of (H) to exo. genomes R1 1478.307 [I-3] RPMH of (I-1) R1 1471.437 [I-4] PPM of (I-1) R1 316665.794 [I-5] RPMU of (I-1) R1 59 (0.0007%) [I-6] Total chimeric reads of (I-1) R1 7.211 [I-7] RPMH of (I-6) R1 7.177 [I-8] PPM of (I-6) R1 1544.584 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 30007749 (100.0000%) [A] Total raw reads R2 8220533 (27.3947%) [B] Total reads after QC of (A) R2 R2 8220533 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 15389388 (93.6033%) [B-3] Total mapped reads of (B-2)x2 R2 1051678 (6.3967%) [B-4] Total unmapped reads of (B-2)x2 R2 7688128 (93.5235%) [F] Total mapped reads of (B-2) R2 532405 (6.4765%) [G] Total unmapped reads of (B-2) R2 38888 (0.4731%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 8181645 (99.5269%) [J] Total mapped reads to host genome ( (B-2)-H) R2 12003 (0.1460%) [I-1] Total mapped reads of (H) to exo. genomes R2 1467.064 [I-3] RPMH of (I-1) R2 1460.124 [I-4] PPM of (I-1) R2 308655.626 [I-5] RPMU of (I-1) R2 54 (0.0007%) [I-6] Total chimeric reads of (I-1) R2 6.600 [I-7] RPMH of (I-6) R2 6.569 [I-8] PPM of (I-6) R2 1388.603 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1.7110 1.7031 366.5113 14 0 14 14.000 679 ALL_R1 fungi Talaromyces 1.32e-04 << 1467.0629 1460.2459 314257.2910 12004 0 12004 12004.000 632493 ALL_R1 viral Lymphocryptovirus 0.0 << 3.9371 3.9185 828.3275 65 52 13 32.212 3003 ALL_R2 bacteria Alcanivorax 4.51e-06 << 1455.9419 1449.0545 306315.5729 11912 0 11912 11912.000 628324 ALL_R2 viral Lymphocryptovirus 0.0 << 0.1222 0.1216 26.1794 1 0 1 1.000 49 R1U fungi Mitosporidium n/a << 6.9662 6.9339 1492.2247 57 0 57 57.000 2815 R1U viral Lymphocryptovirus n/a << 6.1112 6.0823 1285.7437 50 0 50 50.000 2655 R2U viral Lymphocryptovirus n/a