# 2018/02/06 03:53:17 # HUMAN Target (Host) organism # RNA Sequencing source # ENCSR000CUD_1 Replication ID # ENCSR000CUD Sample ID # ENCFF000GHX Read1 # ENCFF000GHY Read2 R1 173994285 (100.0000%) [A] Total raw reads R1 168908456 (97.0770%) [B] Total reads after QC of (A) R1 R1 168908456 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 319215462 (94.4936%) [B-3] Total mapped reads of (B-2)x2 R1 18601450 (5.5064%) [B-4] Total unmapped reads of (B-2)x2 R1 160043496 (94.7516%) [F] Total mapped reads of (B-2) R1 8864960 (5.2484%) [G] Total unmapped reads of (B-2) R1 1512423 (0.8954%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 167396033 (99.1046%) [J] Total mapped reads to host genome ( (B-2)-H) R1 976983 (0.5784%) [I-1] Total mapped reads of (H) to exo. genomes R1 5836.357 [I-3] RPMH of (I-1) R1 5784.098 [I-4] PPM of (I-1) R1 1395 (0.0008%) [I-5] Total chimeric reads of (I-1) R1 8.334 [I-7] RPMH of (I-5) R1 8.259 [I-8] PPM of (I-5) R1 ## ----------------------------------------- R2 173994285 (100.0000%) [A] Total raw reads R2 168908456 (97.0770%) [B] Total reads after QC of (A) R2 R2 168908456 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 319215462 (94.4936%) [B-3] Total mapped reads of (B-2)x2 R2 18601450 (5.5064%) [B-4] Total unmapped reads of (B-2)x2 R2 159171966 (94.2356%) [F] Total mapped reads of (B-2) R2 9736490 (5.7644%) [G] Total unmapped reads of (B-2) R2 1722607 (1.0198%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 167185849 (98.9802%) [J] Total mapped reads to host genome ( (B-2)-H) R2 978303 (0.5792%) [I-1] Total mapped reads of (H) to exo. genomes R2 5851.590 [I-3] RPMH of (I-1) R2 5791.913 [I-4] PPM of (I-1) R2 1140 (0.0007%) [I-5] Total chimeric reads of (I-1) R2 6.819 [I-7] RPMH of (I-5) R2 6.749 [I-8] PPM of (I-5) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMH > 1 # List threshold of P-value < 0.001 # RPMH PPM Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 1424.6725 1411.9158 747185 746506 679 238484.517 74744192 ALL_R1 bacteria Acinetobacter 1.94e-33 906.8944 898.7740 154289 3528 150761 151810.522 15458384 ALL_R1 bacteria Bacillus 0.0 49.2210 48.7803 11073 7138 3935 8239.401 1059637 ALL_R1 bacteria Escherichia 0.0 41.5664 41.1942 9019 2715 6304 6958.054 892413 ALL_R1 bacteria Cutibacterium 0.0 36.0592 35.7363 8291 3493 4798 6036.159 795524 ALL_R1 bacteria Corynebacterium 0.0 12.6398 12.5266 2423 441 1982 2115.846 242799 ALL_R1 bacteria Staphylococcus 0.0 6.9666 6.9042 1601 1002 599 1166.182 155977 ALL_R1 bacteria Bosea 1.91e-73 << 208.3522 206.4866 35639 2245 33394 34877.331 3551716 ALL_R1 fungi Saccharomyces 0.0 << 1403.9792 1389.6608 747496 746787 709 234725.458 74012951 ALL_R2 bacteria Acinetobacter 1.78e-40 922.9926 913.5795 155918 2547 153371 154311.296 15539380 ALL_R2 bacteria Bacillus 0.0 63.2469 62.6019 12822 8903 3919 10573.987 1267901 ALL_R2 bacteria Escherichia 0.0 39.8127 39.4067 7992 2722 5270 6656.122 782071 ALL_R2 bacteria Cutibacterium 0.0 31.0015 30.6854 6519 1784 4735 5183.016 642313 ALL_R2 bacteria Corynebacterium 0.0 12.6999 12.5704 2387 416 1971 2123.247 239007 ALL_R2 bacteria Staphylococcus 0.0 7.0669 6.9949 1528 893 635 1181.494 147843 ALL_R2 bacteria Bosea 2.82e-95 << 208.3736 206.2485 35690 2351 33339 34837.122 3555685 ALL_R2 fungi Saccharomyces 0.0 << 1391.1791 1376.9913 742155 742154 1 232585.466 73490367 ALL_R2 viral Phix174microvirus n/a << 1.9789 1.9612 1012 997 15 331.265 64576 R1U bacteria Mycobacterium n/a RNR2(78);RN7SL2(17);RN7SL1(15);RNR1(14);FN1(12) 1.8918 1.8748 997 996 1 316.672 63376 R1U bacteria Flavobacterium n/a RNR2(78);RNR1(14);RN7SL2(14);FN1(12);RPPH1(8) 1.8810 1.8642 995 994 1 314.872 63478 R1U bacteria Acinetobacter n/a RNR2(78);RNR1(14);RN7SL2(14);FN1(12);RPPH1(8) 1.3523 1.3402 234 9 225 226.364 18388 R1U bacteria Bacillus n/a RNR2(18);RN7SL1(12);RN7SL2(11);TXLNA(6);TAB2(5) << 1.5289 1.5133 258 5 253 255.604 23790 R2U bacteria Bacillus n/a RN7SL2(16);RNR2(14);RN7SK(5);TUBB3(5);RN7SL1(5) 1.2916 1.2784 687 686 1 215.935 60465 R2U bacteria Mycobacterium n/a RNR2(43);RN7SL2(31);RN7SL1(28);RN7SK(15);FN1(15) 1.2895 1.2764 684 683 1 215.592 60122 R2U bacteria Labrenzia n/a RNR2(44);RN7SL2(31);RN7SL1(28);RN7SK(15);FN1(15) 1.2750 1.2620 678 677 1 213.157 59571 R2U bacteria Pseudomonas n/a RNR2(43);RN7SL2(31);RN7SL1(28);RN7SK(15);FN1(15) 1.2723 1.2593 675 674 1 212.705 59338 R2U bacteria Paenibacillus n/a RNR2(43);RN7SL2(31);RN7SL1(27);RN7SK(15);FN1(14) 1.2659 1.2530 673 672 1 211.640 59183 R2U bacteria Acinetobacter n/a RNR2(43);RN7SL2(31);RN7SL1(28);RN7SK(15);FN1(15)