# 2020/03/13 23:02:36 # HUMAN Target (Host) organism # DNA Sequencing source # SRR791550 Replication ID # SRP000547 Sample ID # SRR791550_1.filt Read1 # SRR791550_2.filt Read2 R1 10801807 (100.0000%) [A] Total raw reads R1 8847284 (81.9056%) [B] Total reads after QC of (A) R1 R1 8847284 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 16434374 (92.8781%) [B-3] Total mapped reads of (B-2)x2 R1 1260194 (7.1219%) [B-4] Total unmapped reads of (B-2)x2 R1 8252113 (93.2728%) [F] Total mapped reads of (B-2) R1 595171 (6.7272%) [G] Total unmapped reads of (B-2) R1 78399 (0.8861%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 8768885 (99.1139%) [J] Total mapped reads to host genome ( (B-2)-H) R1 18319 (0.2071%) [I-1] Total mapped reads of (H) to exo. genomes R1 2089.091 [I-3] RPMH of (I-1) R1 2070.579 [I-4] PPM of (I-1) R1 233663.695 [I-5] RPMU of (I-1) R1 127 (0.0014%) [I-6] Total chimeric reads of (I-1) R1 14.483 [I-7] RPMH of (I-6) R1 14.355 [I-8] PPM of (I-6) R1 1619.919 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 10801807 (100.0000%) [A] Total raw reads R2 8847284 (81.9056%) [B] Total reads after QC of (A) R2 R2 8847284 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 16434374 (92.8781%) [B-3] Total mapped reads of (B-2)x2 R2 1260194 (7.1219%) [B-4] Total unmapped reads of (B-2)x2 R2 8182261 (92.4833%) [F] Total mapped reads of (B-2) R2 665023 (7.5167%) [G] Total unmapped reads of (B-2) R2 99371 (1.1232%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 8747913 (98.8768%) [J] Total mapped reads to host genome ( (B-2)-H) R2 18156 (0.2052%) [I-1] Total mapped reads of (H) to exo. genomes R2 2075.466 [I-3] RPMH of (I-1) R2 2052.155 [I-4] PPM of (I-1) R2 182709.241 [I-5] RPMU of (I-1) R2 104 (0.0012%) [I-6] Total chimeric reads of (I-1) R2 11.888 [I-7] RPMH of (I-6) R2 11.755 [I-8] PPM of (I-6) R2 1046.583 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 2087.3806 2068.8835 233472.3657 18304 0 18304 18304.000 1766503 ALL_R1 viral Lymphocryptovirus 0.0 << 2071.5798 2048.3122 182367.0890 18122 0 18122 18122.000 1667242 ALL_R2 viral Lymphocryptovirus 0.0 << 14.3690 14.2417 1607.1634 126 0 126 126.000 12260 R1U viral Lymphocryptovirus n/a << 0.2286 0.2261 20.1266 2 0 2 2.000 162 R2U bacteria Alcanivorax n/a 0.1143 0.1130 10.0633 1 0 1 1.000 98 R2U bacteria Staphylococcus n/a 0.1143 0.1130 10.0633 1 0 1 1.000 36 R2U bacteria Zooshikella n/a << 0.1143 0.1130 10.0633 1 0 1 1.000 63 R2U fungi Coccidioides n/a << 11.3170 11.1899 996.2665 99 0 99 99.000 8712 R2U viral Lymphocryptovirus n/a