# 2020/02/24 22:32:44 # HUMAN Target (Host) organism # RNA Sequencing source # ERR188256 Replication ID # ERR188256 Sample ID # ERR188256_1 Read1 # ERR188256_2 Read2 R1 25636904 (100.0000%) [A] Total raw reads R1 25145031 (98.0814%) [B] Total reads after QC of (A) R1 R1 25145031 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R1 47194129 (93.8438%) [B-3] Total mapped reads of (B-2)x2 R1 3095933 (6.1562%) [B-4] Total unmapped reads of (B-2)x2 R1 23625646 (93.9575%) [F] Total mapped reads of (B-2) R1 1519385 (6.0425%) [G] Total unmapped reads of (B-2) R1 81182 (0.3229%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R1 25063849 (99.6771%) [J] Total mapped reads to host genome ( (B-2)-H) R1 48669 (0.1936%) [I-1] Total mapped reads of (H) to exo. genomes R1 1941.801 [I-3] RPMH of (I-1) R1 1935.531 [I-4] PPM of (I-1) R1 599504.816 [I-5] RPMU of (I-1) R1 36 (0.0001%) [I-6] Total chimeric reads of (I-1) R1 1.436 [I-7] RPMH of (I-6) R1 1.432 [I-8] PPM of (I-6) R1 443.448 [I-9] RPMU of (I-6) R1 ## ----------------------------------------- R2 25636904 (100.0000%) [A] Total raw reads R2 25145031 (98.0814%) [B] Total reads after QC of (A) R2 R2 25145031 (100.0000%) [B-2] Total reads of (B) passed to HISAT2 R2 47194129 (93.8438%) [B-3] Total mapped reads of (B-2)x2 R2 3095933 (6.1562%) [B-4] Total unmapped reads of (B-2)x2 R2 23568483 (93.7302%) [F] Total mapped reads of (B-2) R2 1576548 (6.2698%) [G] Total unmapped reads of (B-2) R2 110832 (0.4408%) [H] Total unmapped reads of (B-2) after running BLASTn with (G) R2 25034199 (99.5592%) [J] Total mapped reads to host genome ( (B-2)-H) R2 48469 (0.1928%) [I-1] Total mapped reads of (H) to exo. genomes R2 1936.112 [I-3] RPMH of (I-1) R2 1927.578 [I-4] PPM of (I-1) R2 437319.547 [I-5] RPMU of (I-1) R2 33 (0.0001%) [I-6] Total chimeric reads of (I-1) R2 1.318 [I-7] RPMH of (I-6) R2 1.312 [I-8] PPM of (I-6) R2 297.748 [I-9] RPMU of (I-6) R2 ## ----------------------------------------- ##=============================================## # List threshold of RPMU > 1 # List threshold of P-value < 0.001 # RPMH PPM RPMU Total Multi_Reads(All_ExoGenus) Uniq_Reads(This_Genus) wTotal Sum_ReadLength Type Exo Genus Pvalue(Uniq) 328.6079 327.5470 101453.2655 10351 4211 6140 8236.179 771950 ALL_R1 bacteria Nocardia 0.0 49.0597 48.9013 15146.5103 2448 2416 32 1229.624 181907 ALL_R1 bacteria Ralstonia 3.46e-04 23.7061 23.6296 7318.9377 672 100 572 594.166 48491 ALL_R1 bacteria Methylobacterium 0.0 22.7534 22.6800 7024.8208 666 104 562 570.289 49129 ALL_R1 bacteria Cutibacterium 0.0 21.5184 21.4489 6643.5170 909 840 69 539.334 67586 ALL_R1 bacteria Escherichia 1.52e-37 11.0552 11.0195 3413.1458 370 126 244 277.086 26842 ALL_R1 bacteria Cupriavidus 0.0 9.4372 9.4067 2913.6015 321 103 218 236.532 23837 ALL_R1 bacteria Lactobacillus 0.0 6.1951 6.1751 1912.6407 287 152 135 155.272 21192 ALL_R1 bacteria Sphingomonas 6.24e-127 4.9231 4.9072 1519.9305 238 126 112 123.391 17464 ALL_R1 bacteria Micrococcus 2.75e-171 3.2692 3.2586 1009.3124 130 53 77 81.938 9482 ALL_R1 bacteria Curvibacter 9.46e-35 3.1356 3.1255 968.0717 121 47 74 78.590 9027 ALL_R1 bacteria Staphylococcus 1.02e-25 2.4082 2.4004 743.5023 111 53 58 60.359 8281 ALL_R1 bacteria Streptococcus 8.88e-25 1.9251 1.9189 594.3559 119 84 35 48.251 8696 ALL_R1 bacteria Bradyrhizobium 5.22e-07 << 15.9504 15.8989 4924.4783 438 49 389 399.779 32124 ALL_R1 fungi Saccharomyces 0.0 << 983.1291 979.9550 303527.8756 24641 0 24641 24641.000 1822580 ALL_R1 viral Lymphocryptovirus 0.0 << 316.2313 314.8375 71428.8112 10334 4192 6142 7916.598 765629 ALL_R2 bacteria Nocardia 0.0 41.8390 41.6546 9450.4024 2458 2426 32 1047.407 179188 ALL_R2 bacteria Ralstonia 3.08e-04 23.4556 23.3522 5298.0276 668 97 571 587.191 48237 ALL_R2 bacteria Methylobacterium 0.0 21.1052 21.0121 4767.1340 665 211 454 528.351 49030 ALL_R2 bacteria Cutibacterium 0.0 19.5300 19.4439 4411.3433 915 848 67 488.918 67694 ALL_R2 bacteria Escherichia 2.59e-35 11.3896 11.3394 2572.6234 394 133 261 285.129 28820 ALL_R2 bacteria Cupriavidus 0.0 9.0099 8.9702 2035.1162 312 98 214 225.556 23141 ALL_R2 bacteria Lactobacillus 0.0 6.3292 6.3013 1429.6142 292 149 143 158.447 21479 ALL_R2 bacteria Sphingomonas 3.60e-146 4.2436 4.2249 958.5228 240 150 90 106.235 17742 ALL_R2 bacteria Micrococcus 1.39e-101 2.9772 2.9641 672.4773 112 40 72 74.532 8329 ALL_R2 bacteria Staphylococcus 3.25e-24 2.4942 2.4832 563.3842 107 45 62 62.441 7954 ALL_R2 bacteria Streptococcus 5.29e-30 2.4465 2.4357 552.6112 121 65 56 61.247 8862 ALL_R2 bacteria Curvibacter 9.70e-16 1.7687 1.7609 399.5056 114 80 34 44.278 8396 ALL_R2 bacteria Bradyrhizobium 2.00e-06 << 15.6874 15.6182 3543.3900 442 59 383 392.721 32527 ALL_R2 fungi Saccharomyces 0.0 << 980.4987 976.1770 221470.3335 24546 0 24546 24546.000 1815366 ALL_R2 viral Lymphocryptovirus 0.0 << 0.1596 0.1591 49.2720 4 0 4 4.000 271 R1U bacteria Nocardia n/a 0.0676 0.0674 20.8667 10 9 1 1.694 600 R1U bacteria Paenibacillus n/a 0.0399 0.0398 12.3180 1 0 1 1.000 74 R1U bacteria Corynebacterium n/a << 0.0399 0.0398 12.3180 2 1 1 1.000 149 R1U fungi Mitosporidium n/a 0.0399 0.0398 12.3180 2 1 1 1.000 116 R1U fungi Marssonina n/a 0.0399 0.0398 12.3180 1 0 1 1.000 57 R1U fungi Cladophialophora n/a << 0.5586 0.5568 172.4520 14 0 14 14.000 1024 R1U viral Lymphocryptovirus n/a << 0.0799 0.0795 18.0453 2 0 2 2.000 150 R2U bacteria Nocardia n/a 0.0399 0.0398 9.0227 1 0 1 1.000 74 R2U bacteria Lachnoclostridium n/a 0.0399 0.0398 9.0227 1 0 1 1.000 48 R2U bacteria Nitrospirillum n/a 0.0399 0.0398 9.0227 1 0 1 1.000 46 R2U bacteria Anoxybacillus n/a 0.0399 0.0398 9.0227 1 0 1 1.000 39 R2U bacteria Hymenobacter n/a << 0.1049 0.1045 23.7025 3 1 2 2.627 119 R2U fungi Aspergillus n/a 0.0650 0.0647 14.6799 2 1 1 1.627 114 R2U fungi Clavispora n/a 0.0399 0.0398 9.0227 1 0 1 1.000 75 R2U fungi Cladophialophora n/a 0.0399 0.0398 9.0227 1 0 1 1.000 61 R2U fungi Tremella n/a 0.0399 0.0398 9.0227 1 0 1 1.000 51 R2U fungi Sugiyamaella n/a 0.0399 0.0398 9.0227 1 0 1 1.000 43 R2U fungi Thielavia n/a 0.0399 0.0398 9.0227 1 0 1 1.000 41 R2U fungi Coniophora n/a 0.0399 0.0398 9.0227 1 0 1 1.000 38 R2U fungi Ogataea n/a 0.0399 0.0398 9.0227 1 0 1 1.000 38 R2U fungi Malassezia n/a << 0.3595 0.3579 81.2040 9 0 9 9.000 671 R2U viral Lymphocryptovirus n/a 0.0399 0.0398 9.0227 1 0 1 1.000 39 R2U viral Alphabaculovirus n/a